【摘要】：目的:研究不同劑量的人外周血γδT細胞對裸鼠人肝癌細胞(SMMC-7721)移植瘤模型的免疫治療作用。方法:(1)用人肝癌細胞株SMMC-7721接種BALB/c裸鼠皮下,建立肝癌裸鼠模型。(2)從健康人外周血中提取單核細胞,體外特異性擴增γδT細胞。(3)將已建立的肝癌裸鼠模型隨機分為5組,陽性對照組為5-氟尿嘧啶(5-Fu),陰性對照組為生理鹽水,治療組用不同劑量的γδT細胞(1×105、5×10~5及25×10~5)分別注入裸鼠尾靜脈,陽性對照組用5-Fu裸鼠腹腔注射,陰性對照組用生理鹽水裸鼠尾靜脈注射。觀察不同劑量的γδT細胞對腫瘤的抑制效果,包括治療前后的體重、食物攝取量及生長狀況等,并與陽性對照組和陰性對照組比較腫瘤體積(TV)、相對腫瘤體積(RTV)和相對腫瘤增殖率[T/C(%)]變化。結果:不同劑量的γδT細胞對裸鼠移植瘤的生長有不同程度的抑制,RTV與生理鹽水陰性對照組比較差異有統(tǒng)計學意義(P0.05);與5-Fu陽性對照組比較,TV增長明顯低于5-Fu陽性對照組,RTV各劑量組抑制程度相似,均略高于5-Fu陽性對照組,T/C(%)各劑量組比5-Fu對照組的相對腫瘤增殖率稍低,但無顯著性差異。結論:人外周血γδT細胞對肝癌裸鼠移植瘤具有顯著的抑瘤作用,為建立肝癌免疫治療新方法提供實驗依據。
[Abstract]:Aim: to study the immunotherapy of human peripheral blood 緯 未 T cells in nude mice transplanted with human hepatoma cells (SMMC-7721). Methods: (1) Human hepatoma cell line SMMC-7721 was inoculated subcutaneously into BALB/c nude mice to establish hepatoma nude mice model. (2) Monocyte was extracted from healthy human peripheral blood and 緯 未 T cells were amplified in vitro. (3) the established nude mice were randomly divided into 5 groups. The positive control group was 5-fluorouracil (5-Fu), the negative control group was normal saline, the treatment group was injected into the tail vein of nude mice with different doses of 緯 未 T cells (1 脳 105 T5 脳 10 5 and 25 脳 10 5), and the positive control group was injected intraperitoneally with 5-Fu. The negative control group was injected with saline into tail vein of nude mice. The inhibitory effects of different doses of 緯 未 T cells on tumor were observed, including body weight, food intake and growth status before and after treatment. The changes of tumor volume (TV), relative to tumor volume (RTV) and tumor proliferation rate [T / C (%)] were compared with those of positive control group and negative control group. Results: different doses of 緯 未 T cells inhibited the growth of xenografts in nude mice to varying degrees (P0.05), and the growth of TV-positive cells was significantly lower than that of 5-Fu positive controls (P0.05). The inhibition degree of RTV in each dose group was similar, The relative tumor proliferation rate of each dose group was slightly lower than that of 5-Fu group, but there was no significant difference. Conclusion: human peripheral blood 緯 未 T cells have a significant inhibitory effect on hepatoma xenografts in nude mice and provide experimental evidence for the establishment of a new immunotherapy method for liver cancer.
【作者單位】： 吉林省人民醫(yī)院;吉林省腫瘤防治研究所;
【基金】：吉林省科技發(fā)展計劃項目(20160101197JC)資助
【分類號】：R735.7

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