【摘要】：目的尋找與卵巢漿液性癌相關(guān)的microRNA,積累以國內(nèi)患者為數(shù)據(jù)來源的microRNA表達(dá)譜資料,并初步探討其臨床意義。方法利用microRNA芯片,篩查卵巢漿液性癌組織及正常卵巢組織中差異表達(dá)的microRNA,用實(shí)時熒光定量PCR在58份卵巢組織標(biāo)本中進(jìn)行驗(yàn)證,并分析microRNA表達(dá)水平與卵巢漿液性癌臨床病理特征之間的關(guān)系。結(jié)果與正常卵巢組織比較,芯片結(jié)果顯示卵巢漿液性癌有9個microRNA明顯上調(diào),分別是hsa-mi R-141-3p、hsa-mi R-200a-3p、hsa-mi R-200b-3p、hsa-mi R-200c-3p、hsa-mi R-224-5p、hsa-mi R-7-5p、hsa-mi R-142-3p、hsa-mi R-21-5p、hsa-mi R-142-5p,6個microRNA明顯下調(diào),分別是hsa-mi R-424-5p、hsa-mi R-214-3p、hsa-mi R-125b-5p、hsa-let-7b-5p、hsa-mi R-199a-5p、hsa-mi R-100-5p。實(shí)時熒光定量PCR驗(yàn)證結(jié)果與芯片結(jié)果一致。對差異microRNA進(jìn)行分層分析發(fā)現(xiàn),與卵巢漿液性癌Ⅰ-Ⅱ期、淋巴結(jié)未轉(zhuǎn)移患者比較,Ⅲ-Ⅳ期、淋巴結(jié)有轉(zhuǎn)移患者的hsa-mi R-21-5p表達(dá)均增高,hsa-mi R-125b-5p表達(dá)均降低(P0.05);而在高級別與低級別卵巢癌患者之間microRNA的表達(dá)水平差異均無統(tǒng)計(jì)學(xué)意義(P均0.05)。結(jié)論卵巢漿液性癌中異常表達(dá)的microRNA可能與腫瘤的發(fā)生發(fā)展相關(guān)。
[Abstract]:Objective to search for microRNAs associated with ovarian serous carcinoma and to accumulate microRNA expression profiles from domestic patients, and to explore its clinical significance. Methods the differentially expressed microRNAs in ovarian serous carcinoma tissues and normal ovarian tissues were screened by microRNA microarray and verified by real-time fluorescence quantitative PCR in 58 ovarian tissues. The relationship between the expression of microRNA and the clinicopathological features of ovarian serous carcinoma was analyzed. 緇撴灉涓庢甯稿嵉宸㈢粍緇囨瘮杈，

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