Cytokeratin和CD133在肺腺癌患者外周血中的聯(lián)合檢測(cè)及臨床病理分析
[Abstract]:Objective to detect the expression of cytokeratin CK (positive circulating tumor cells) and CD133 gene (stem cell marker of lung cancer) in peripheral blood of patients with lung adenocarcinoma and to evaluate their clinical value in evaluating the prognosis of lung adenocarcinoma. Methods from June 2014 to June 2016, 178 patients with lung adenocarcinoma were selected from cardiothoracic surgery department of Kunming General Hospital and Cardiothoracic surgery Department of the first people's Hospital of Xuanwei City. The median age was 59 years old, including 78 males and 100 females. The peripheral blood samples of the patients were collected 10 mL before operation, and the expression of CD133 was detected by CT-PCR and CK (immunocytochemical method). Immunohistochemical method was used to detect the expression of CK-Vimentin (Vim) and Ki-67-pCD133 in lung adenocarcinoma, and the relationship between the expression of CK and CD133 in peripheral blood was analyzed. According to the detection results of CK and CD133 in peripheral blood, the patients were divided into CK/CD133 double positive group and non double positive group. The difference of clinicopathological factors between the two groups was analyzed statistically. Results the positive rate of CTC (CK expression in peripheral blood of 178 patients with lung adenocarcinoma was 48.3% (86 / 178), which was positively correlated with Ki-67Vim expression (P0.05), and the positive rate of CD133 expression in peripheral blood was 66.9% (119/ 178), which was significantly correlated with the expression of Vim-CD133 in cancer tissue (P0.05), and in peripheral blood CK/CD133 double positive group (53 cases) (P0.05). The lymph node metastasis rate in the Shuangyang group was significantly higher than that in the control group (29.8%). Histological differentiation grade, clinical stage and metastasis rate of lung adenocarcinoma decreased significantly (P0.05). Conclusion the positive expression of CK and CD133 in peripheral blood of patients with lung adenocarcinoma suggests that there are circulating tumor cells with dry characteristics in peripheral blood, indicating poor prognosis. The combined detection of CK and CD133 can be used to monitor the prognosis of patients with lung adenocarcinoma and to guide the treatment.
【作者單位】: 成都軍區(qū)昆明總醫(yī)院心胸外科;成都軍區(qū)昆明總醫(yī)院病理實(shí)驗(yàn)科;云南省第二人民醫(yī)院急診科;云南省宣威市第一人民醫(yī)院心胸外科;
【基金】:國(guó)家教育部重點(diǎn)實(shí)驗(yàn)室開放課題(2013jszl13) 云南省應(yīng)用基礎(chǔ)青年基金(2013FD091)~~
【分類號(hào)】:R734.2
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