發(fā)布時間：2018-07-21 16:11

【摘要】：目的:探討Hedgehog信號通路阻斷劑GANT61對胃癌細胞株MC的細胞毒性及其對MC凋亡和遷移能力的影響。方法:采用不同濃度的GANT61處理胃癌細胞MC后,MTT法檢測GANT61對胃癌細胞株MC的細胞毒性;流式細胞術檢測GANT61對MC細胞周期以及凋亡的影響;Western blot驗證GANT61對胃癌細胞株MC中Gli1蛋白表達的影響;細胞劃痕實驗檢測GANT61對胃癌細胞株MC遷移能力的影響。結果:采用GANT61濃度20umol/L處理細胞時,細胞活性下降50%,MC細胞凋亡率增加(實驗組6.60±1.04%vs對照組2.47±0.47%);GANT61能夠下調胃癌細胞株MC中Gli1蛋白的表達,另外GANT61能有效抑制胃癌細胞株MC的遷移能力。結論:GANT61通過阻斷Hedgehog信號通路,表現(xiàn)出顯著的細胞毒性,有效抑制胃癌細胞株MC的遷移能力并誘導胃癌細胞株MC的細胞周期阻滯及細胞凋亡,為胃癌的靶向治療提供一種新的治療選擇與思路。
[Abstract]:Aim: to investigate the cytotoxicity of Hedgehog signaling blocker GANT61 on gastric cancer cell line MC and its effect on apoptosis and migration. Methods: the cytotoxicity of GANT61 to gastric cancer cell line MC was detected by MTT assay after treated with different concentrations of GANT61. The effect of GANT61 on cell cycle and apoptosis was detected by flow cytometry. Western blot was used to test the effect of GANT61 on the expression of Gli1 protein in gastric cancer cell line MC, and the effect of GANT61 on the migration ability of gastric cancer cell line was detected by cell scratch assay. Results: when treated with 20 umol / L GANT61, the cell activity decreased 50% and the apoptosis rate of MC increased (experimental group 6.60 鹵1.04%vs 2.47 鹵0.47%). GANT61 could down-regulate the expression of Gli1 protein in gastric cancer cell line MC, and GANT61 could effectively inhibit the migration ability of gastric cancer cell line MC. Conclusion by blocking the Hedgehog signaling pathway, the cell cycle arrest and apoptosis of gastric cancer cell line MC can be effectively inhibited. To provide a new therapeutic choice and thinking for the targeted treatment of gastric cancer.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R735.2

【參考文獻】

相關期刊論文 前3條

1 孟鵬鵬;閆慧明;馮廣躍;;Shh、Gli-1在經(jīng)低濃度MNNG轉化的GES-1細胞中的表達及意義[J];中國醫(yī)療前沿;2011年02期

2 Jin-Woo Park;Young-Jin Song;Hyo-Yung Yun;Joo-Seung Park;Taisun Hyun;Sei-Jin Youn;Yong-Dae Kim;Jong-Won Kang;Heon Kim;;Kimchi and soybean pastes are risk factors of gastric cancer[J];World Journal of Gastroenterology;2005年21期

3 李鶴;;人乳頭瘤病毒檢測在宮頸CIN診斷及隨訪中的意義[J];中國醫(yī)學創(chuàng)新;2014年01期

，

本文編號：2136066