【摘要】：目的:探討Hedgehog信號(hào)通路阻斷劑GANT61對(duì)胃癌細(xì)胞株MC的細(xì)胞毒性及其對(duì)MC凋亡和遷移能力的影響。方法:采用不同濃度的GANT61處理胃癌細(xì)胞MC后,MTT法檢測(cè)GANT61對(duì)胃癌細(xì)胞株MC的細(xì)胞毒性;流式細(xì)胞術(shù)檢測(cè)GANT61對(duì)MC細(xì)胞周期以及凋亡的影響;Western blot驗(yàn)證GANT61對(duì)胃癌細(xì)胞株MC中Gli1蛋白表達(dá)的影響;細(xì)胞劃痕實(shí)驗(yàn)檢測(cè)GANT61對(duì)胃癌細(xì)胞株MC遷移能力的影響。結(jié)果:采用GANT61濃度20umol/L處理細(xì)胞時(shí),細(xì)胞活性下降50%,MC細(xì)胞凋亡率增加(實(shí)驗(yàn)組6.60±1.04%vs對(duì)照組2.47±0.47%);GANT61能夠下調(diào)胃癌細(xì)胞株MC中Gli1蛋白的表達(dá),另外GANT61能有效抑制胃癌細(xì)胞株MC的遷移能力。結(jié)論:GANT61通過阻斷Hedgehog信號(hào)通路,表現(xiàn)出顯著的細(xì)胞毒性,有效抑制胃癌細(xì)胞株MC的遷移能力并誘導(dǎo)胃癌細(xì)胞株MC的細(xì)胞周期阻滯及細(xì)胞凋亡,為胃癌的靶向治療提供一種新的治療選擇與思路。
[Abstract]:Aim: to investigate the cytotoxicity of Hedgehog signaling blocker GANT61 on gastric cancer cell line MC and its effect on apoptosis and migration. Methods: the cytotoxicity of GANT61 to gastric cancer cell line MC was detected by MTT assay after treated with different concentrations of GANT61. The effect of GANT61 on cell cycle and apoptosis was detected by flow cytometry. Western blot was used to test the effect of GANT61 on the expression of Gli1 protein in gastric cancer cell line MC, and the effect of GANT61 on the migration ability of gastric cancer cell line was detected by cell scratch assay. Results: when treated with 20 umol / L GANT61, the cell activity decreased 50% and the apoptosis rate of MC increased (experimental group 6.60 鹵1.04%vs 2.47 鹵0.47%). GANT61 could down-regulate the expression of Gli1 protein in gastric cancer cell line MC, and GANT61 could effectively inhibit the migration ability of gastric cancer cell line MC. Conclusion by blocking the Hedgehog signaling pathway, the cell cycle arrest and apoptosis of gastric cancer cell line MC can be effectively inhibited. To provide a new therapeutic choice and thinking for the targeted treatment of gastric cancer.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R735.2

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本文編號(hào)：2136066