【摘要】：動(dòng)物組織樣品的分析是從分子層面對(duì)其本身及其代謝產(chǎn)物的分析,在醫(yī)學(xué)、食品安全等領(lǐng)域的研究中具有重要意義。但是傳統(tǒng)的分析方法大都需要復(fù)雜的研磨、萃取、分離等預(yù)處理過(guò)程,不僅冗長(zhǎng)而耗時(shí),更嚴(yán)重的是會(huì)損失組織樣品內(nèi)部一些重要的物質(zhì)信息,從而導(dǎo)致分析效率低下。因此,開(kāi)發(fā)出一種“綠色、快速、無(wú)損”的組織樣品檢測(cè)分析方法就顯得非常有意義。內(nèi)部萃取電噴霧電離質(zhì)譜技術(shù)(iEESI-MS)是近年興起的常壓質(zhì)譜技術(shù),其獨(dú)特的優(yōu)勢(shì)是可以在無(wú)需任何樣品前處理的條件下直接獲取復(fù)雜基體固形樣品內(nèi)部組分的化學(xué)信息,具有操作簡(jiǎn)單、分析速度快、高選擇性、高靈敏度、高通量等優(yōu)點(diǎn)。本文著重研究了iEESI-MS技術(shù)對(duì)肺癌組織與癌旁組織中氨基酸的定性定量分析、肉類中痕量瘦肉精成分的鑒定與微量肉類品質(zhì)鑒定的應(yīng)用。本文主要研究?jī)?nèi)容包括以下三個(gè)方面:1.采用iEESI-MS技術(shù)對(duì)肺癌組織和癌旁組織中氨基酸的分析研究。1)無(wú)需任何樣品預(yù)處理,iEESI-MS技術(shù)對(duì)肺癌組織和癌旁組織中氨基酸的定性定量分析,同時(shí)所得質(zhì)譜數(shù)據(jù)通過(guò)主成分分析(PCA)實(shí)現(xiàn)了癌癥組織與癌旁組織的快速區(qū)分,為臨床組織樣品的快速檢測(cè)提供了一種有力的技術(shù)支撐;2)將iEESI-MS與DESI-MS技術(shù)的分析性能進(jìn)行對(duì)比。結(jié)果表明iEESI-MS技術(shù)具有較好的定量能力,能同時(shí)實(shí)現(xiàn)組織表面和內(nèi)部待測(cè)物的萃取、電離過(guò)程,獲得更加豐富的化學(xué)信息,并且靈敏度更高、檢測(cè)限更低。2.iEESI-MS技術(shù)對(duì)肉類中非法添加成分的分析研究。開(kāi)發(fā)了能直接分析肉類產(chǎn)品中“瘦肉精”成分的iEESI-MS技術(shù)質(zhì)譜檢測(cè)平臺(tái)。利用該分析平臺(tái)對(duì)江西省體育科學(xué)研究所從屠宰場(chǎng)獲得的豬肉、羊肉、牛肉和超市購(gòu)買牛肉樣品分別進(jìn)行iEESI-MS分析,實(shí)現(xiàn)了對(duì)肉類中痕量“瘦肉精”物質(zhì)的準(zhǔn)確、快速分析和鑒定。3.微量肉類品質(zhì)的iEESI-MS分析。采用iEESI-MS質(zhì)譜技術(shù)對(duì)微量瘦豬肉和肥豬肉組織樣品進(jìn)行質(zhì)譜分析,獲得其組織樣品的一級(jí)指紋譜圖以及實(shí)現(xiàn)其肉品中的營(yíng)養(yǎng)組分的成分鑒定,并結(jié)合主成分分析(PCA)法對(duì)兩種組織樣品的質(zhì)譜數(shù)據(jù)進(jìn)行區(qū)分建立判別模型。結(jié)果表明,iEESI-MS能夠直接快速地獲取復(fù)雜基體動(dòng)物組織樣品內(nèi)部豐富的物質(zhì)信息,可以為食品中營(yíng)養(yǎng)成分的分析和研究提供一種可靠的檢測(cè)工具,同時(shí)也在肉類食品安全檢測(cè)中具有重要的現(xiàn)實(shí)意義。
[Abstract]:The analysis of animal tissue samples is the analysis of itself and its metabolites at the molecular level, which is of great significance in the field of medicine, food safety and so on. However, traditional analytical methods require complex pretreatment processes, such as grinding, extraction, separation and so on, which are not only lengthy and time-consuming, but also lead to the loss of some important material information in tissue samples, which leads to low efficiency of analysis. Therefore, it is of great significance to develop a "green, fast, non-destructive" method for tissue sample detection and analysis. Internal extraction electrospray ionization mass spectrometry (iEESI-MS) is a recently developed atmospheric mass spectrometry technique. Its unique advantage is that it can directly obtain the chemical information of the internal components of complex matrix solid samples without any sample pretreatment. It has the advantages of simple operation, high analysis speed, high selectivity, high sensitivity and high throughput. In this paper, the application of iEESI-MS to the qualitative and quantitative analysis of amino acids in lung cancer tissues and adjacent tissues, the identification of trace lean components in meat and the identification of trace meat quality were studied. The main contents of this paper include the following three aspects: 1. Analysis of amino acids in lung cancer tissues and adjacent tissues by iEESI-MS. 1) the qualitative and quantitative analysis of amino acids in lung cancer tissues and adjacent tissues without any sample pretreatment. At the same time, the mass spectrum data obtained by principal component analysis (PCA) can quickly distinguish cancer tissues from adjacent tissues, which provides a powerful technical support for the rapid detection of clinical tissue samples. The analytical performance of iEESI-MS and DESI-MS is compared with that of DESI-MS. The results show that iEESI-MS has better quantitative ability, can simultaneously realize the extraction and ionization process of the substance to be tested on the surface and inside of the tissue, obtain more abundant chemical information, and have higher sensitivity. The detection limit is lower. 2. Analysis of illegally added ingredients in meat by iEESI-MS. An iEESI-MS mass spectrometry platform was developed for the direct analysis of "lean meatine" in meat products. Using the platform, the samples of pork, mutton, beef and beef purchased from slaughterhouses were analyzed by iEESI-MS in Jiangxi Institute of Sports Science, respectively, and the trace "lean meatine" in meat was accurately analyzed. Rapid analysis and identification. Analysis of trace meat quality by iEESI-MS. The tissue samples of lean pork and fat pork were analyzed by iEESI-MS mass spectrometry. The first order fingerprint of the tissue samples and the identification of nutrient components in the meat were obtained. Combined with principal component analysis (PCA), a discriminant model was established to distinguish the mass spectrum data of two kinds of tissue samples. The results show that iEESI-MS can directly and quickly obtain abundant material information from the samples of complex matrix animal tissues, and can provide a reliable tool for the analysis and research of nutritional components in food. At the same time, it has important practical significance in meat food safety detection.
【學(xué)位授予單位】：東華理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R73-3;TS251.7;O657.63

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