毛細管電泳技術(shù)在T細胞淋巴瘤診斷中的應用
發(fā)布時間：2018-07-16 18:27
【摘要】：目的淋巴瘤的發(fā)生被認為是B或T淋巴細胞被阻斷在其分化過程中某一階段而造成淋巴細胞的克隆性增生所致。多數(shù)淋巴系統(tǒng)增生性疾病通過組織形態(tài)學和免疫表型分析能夠明確診斷,但對于T細胞淋巴瘤(T cell Lymphoma,TCL)的診斷,僅憑形態(tài)學與免疫表型分析難以明確。本研究旨在探討毛細管電泳技術(shù)在TCL診斷中的應用價值。方法回顧性分析2014-01-04-2015-06-30齊魯醫(yī)學檢驗所經(jīng)形態(tài)學和免疫組織化學診斷的TCL和不能明確診斷的淋巴組織增生性病變共63例,利用毛細管電泳基因片段分析技術(shù)分析基因重排產(chǎn)物的克隆性。結(jié)果 25例TCL中,T細胞受體-G(T-cell receptor G,TCRG)、TCRB、TCRD基因重排的檢出率分別為88.0%(22/25)、72.0%(18/25)和20.0%(5/25),三者聯(lián)合檢出率為96.0%(24/25)。38例未明確診斷的淋巴組織增生性病變中,21例為多克隆性增生。結(jié)論毛細管電泳基因片段分析對TCL的診斷能夠提供遺傳學的支持,并且對形態(tài)學及免疫組化不典型或疑難病例可以提供診斷方向,利于TCL的病理診斷及鑒別診斷。
[Abstract]:Objective Lymphoma is thought to be due to the clonal proliferation of lymphocytes due to the blocking of B or T lymphocytes at a certain stage of their differentiation. Most lymphoproliferative diseases can be diagnosed by histomorphologic and immunophenotypic analysis, but the diagnosis of T cell lymphoma (T cell lymphoma) is difficult to be determined by morphological and immunophenotypic analysis alone. The purpose of this study was to explore the value of capillary electrophoresis in the diagnosis of TCL. Methods 63 cases of lymphoproliferative lesions diagnosed by morphology and immunohistochemistry in Qilu Medical examination Institute 2014-01-04-2015-06-30 were retrospectively analyzed. The clonality of gene rearrangement products was analyzed by capillary electrophoresis gene fragment analysis. Results the positive rates of T-cell receptor -G (T-cell receptor) TCRD gene rearrangement were 88.0% (22 / 25) 72.0% (18 / 25) and 20.0% (5 / 25), respectively. The combined detection rate was 96.0% (24 / 25) .38 cases of undiagnosed lymphoproliferative lesions were polyclonal hyperplasia. Conclusion Capillary electrophoresis gene fragment analysis can provide genetic support for the diagnosis of TCL, and can provide diagnostic direction for atypical or difficult cases of morphology and immunohistochemistry, which is helpful to the pathological diagnosis and differential diagnosis of TCL.
【作者單位】： 山東省交通醫(yī)院病理科;天津醫(yī)科大學附屬腫瘤醫(yī)院病理科;齊魯醫(yī)學檢驗所病理科;
【基金】：山東省交通運輸科技計劃(2013B24)
【分類號】：R733.1
，
本文編號：2127260
[Abstract]:Objective Lymphoma is thought to be due to the clonal proliferation of lymphocytes due to the blocking of B or T lymphocytes at a certain stage of their differentiation. Most lymphoproliferative diseases can be diagnosed by histomorphologic and immunophenotypic analysis, but the diagnosis of T cell lymphoma (T cell lymphoma) is difficult to be determined by morphological and immunophenotypic analysis alone. The purpose of this study was to explore the value of capillary electrophoresis in the diagnosis of TCL. Methods 63 cases of lymphoproliferative lesions diagnosed by morphology and immunohistochemistry in Qilu Medical examination Institute 2014-01-04-2015-06-30 were retrospectively analyzed. The clonality of gene rearrangement products was analyzed by capillary electrophoresis gene fragment analysis. Results the positive rates of T-cell receptor -G (T-cell receptor) TCRD gene rearrangement were 88.0% (22 / 25) 72.0% (18 / 25) and 20.0% (5 / 25), respectively. The combined detection rate was 96.0% (24 / 25) .38 cases of undiagnosed lymphoproliferative lesions were polyclonal hyperplasia. Conclusion Capillary electrophoresis gene fragment analysis can provide genetic support for the diagnosis of TCL, and can provide diagnostic direction for atypical or difficult cases of morphology and immunohistochemistry, which is helpful to the pathological diagnosis and differential diagnosis of TCL.
【作者單位】： 山東省交通醫(yī)院病理科;天津醫(yī)科大學附屬腫瘤醫(yī)院病理科;齊魯醫(yī)學檢驗所病理科;
【基金】：山東省交通運輸科技計劃(2013B24)
【分類號】：R733.1
，
本文編號：2127260
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