【摘要】：目的:探討咖啡酸苯乙酯(phenethyl caffeate,CAPE)對(duì)肝細(xì)胞生長(zhǎng)因子(hepatocyte growth factor,HGF)誘導(dǎo)的人肝癌細(xì)胞系(Hep G2細(xì)胞)侵襲和遷移的抑制作用及機(jī)制。方法:將實(shí)驗(yàn)分為對(duì)照組(HGF=0 ng/m L;CAPE=0μmol/L)、誘導(dǎo)組(HGF=20 ng/m L;CAPE=0μmol/L)和荷藥組(HGF=20 ng/m L;CAPE分別為2.5、5.0、7.5、10.0μmol/L),測(cè)定細(xì)胞黏附率,Transwell小室模擬細(xì)胞侵襲和遷移能力以及劃痕實(shí)驗(yàn)測(cè)定細(xì)胞遷移率,蛋白免疫印跡(Western blot)法檢測(cè)ELMO1、MAP4K4和FNDC3B表達(dá)。結(jié)果:與對(duì)照組比較,誘導(dǎo)組表現(xiàn)出顯著的促進(jìn)細(xì)胞黏附和遷移作用,荷藥組中表現(xiàn)出對(duì)HGF誘導(dǎo)的細(xì)胞黏附和遷移具有顯著的抑制作用(P0.05)。Transwell小室實(shí)驗(yàn)結(jié)果表明CAPE對(duì)HGF誘導(dǎo)的Hep G2細(xì)胞侵襲和遷移作用有抑制作用。Western blot結(jié)果顯示,誘導(dǎo)組和對(duì)照組相比ELMO1和MAP4K4表達(dá)顯著上調(diào),荷藥組和誘導(dǎo)組相比ELMO1和MAP4K4表達(dá)顯著下調(diào)(P0.05);誘導(dǎo)組表達(dá)FNDC3B較對(duì)照組顯著下降(P0.05),荷藥組FNDC3B表達(dá)量較誘導(dǎo)組增加,但結(jié)果不顯著(P0.05)。結(jié)論:CAPE能夠抑制HGF誘導(dǎo)的Hep G2細(xì)胞的黏附、侵襲和遷移。CAPE通過上調(diào)FNDC3B和下調(diào)ELMO1、MAP4KE的表達(dá),抑制Hep G2細(xì)胞的侵襲和遷移能力。
[Abstract]:Aim: to investigate the inhibitory effect of phenethyl caffeate (Cape) on the invasion and migration of human hepatoma cell line (Hep G2) induced by hepatocyte growth factor (hepatocyte growth). Methods: the experiment was divided into three groups: control group (HGF0 ng/m / L CAPE-0 渭 mol / L), induction group (20 ng/m / L CAPE-0 渭 mol / L) and drug bearing group (20 ng/m / L HGFGF-20 ng/m / L CAPE = 2.5 ng/m / L). The cell adhesion rate and the ability of simulating cell invasion and migration in the transwell compartment were measured by scratch assay. The expression of ELMO1 map4K4 and FNDC3B was detected by Western blot. Results: compared with the control group, the induction group showed significant effects on promoting cell adhesion and migration. The cell adhesion and migration induced by HGF-induced cell adhesion and migration were significantly inhibited in the drug group (P0.05). The results of Transwell chamber experiment showed that Cape could inhibit the invasion and migration of HepG2 cells induced by HGF. Western blot showed that Cape could inhibit the invasion and migration of HGF-induced Hep G2 cells. The expression of ELMO1 and MAP4K4 was significantly up-regulated in the induced group compared with the control group, the expression of ELMO1 and MAP4K4 was down-regulated significantly (P0.05), the expression of FNDC3B in the induced group was significantly lower than that in the control group (P0.05), the expression of FNDC3B in the drug group was higher than that in the induced group, but the results were not significant (P0.05). Conclusion:% Cape can inhibit the adhesion, invasion and migration of HGF induced Hep G2 cells. Cape can inhibit the invasion and migration of Hep G2 cells by upregulating FNDC3B and down-regulating the expression of ELMO1 MAP4KE.
【作者單位】： 南京農(nóng)業(yè)大學(xué)食品科技學(xué)院;中國(guó)農(nóng)業(yè)科學(xué)院蜜蜂研究所;
【基金】：中國(guó)農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程專項(xiàng)(CAAS-ASTIP-2015-IAR)
【分類號(hào)】：TS201.4;R735.7

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 劉桂芳;;Cape隔熱材料公司的新產(chǎn)品[J];建材工業(yè)信息;1985年17期

2 ;[J];;年期

相關(guān)會(huì)議論文 前2條

1 劉瑞;陳宏莉;柏樺;張曉迪;梁欣;海春旭;;蜂膠提取物CAPE對(duì)大鼠肺纖維化的干預(yù)作用研究[A];第十一次中國(guó)生物物理學(xué)術(shù)大會(huì)暨第九屆全國(guó)會(huì)員代表大會(huì)摘要集[C];2009年

2 寧顯玲;馬小艷;王孝偉;張志麗;劉俊義;;咖啡酸苯乙酯(CAPE)類似物的合成及其抗腫瘤活性評(píng)估[A];2011年全國(guó)藥物化學(xué)學(xué)術(shù)會(huì)議——藥物的源頭創(chuàng)新論文摘要集[C];2011年

相關(guān)博士學(xué)位論文 前2條

1 郭川;CAPE在神經(jīng)膠質(zhì)瘤異常Wnt/β-catenin通路中的作用及機(jī)制研究[D];重慶醫(yī)科大學(xué);2009年

2 楊剛;咖啡酸苯乙酯對(duì)血管平滑肌細(xì)胞增殖的影響及其對(duì)動(dòng)脈內(nèi)膜損傷后血管再狹窄的防治作用及機(jī)制[D];華中科技大學(xué);2007年

相關(guān)碩士學(xué)位論文 前5條

1 劉明明;基于CAPE-OPEN熱力學(xué)計(jì)算系統(tǒng)開發(fā)[D];青島科技大學(xué);2016年

2 茍靜;咖啡酸對(duì)硝基苯乙酯在Caco-2細(xì)胞的轉(zhuǎn)運(yùn)特性及其在大鼠的體內(nèi)過程分析[D];西南大學(xué);2016年

3 劉天亮;CAPE衍生物對(duì)大鼠心肌I/RI的保護(hù)作用研究[D];西南大學(xué);2013年

4 王小強(qiáng);CAPE對(duì)人宮頸癌細(xì)胞放射增敏作用及機(jī)理研究[D];蘇州大學(xué);2008年

5 蔣超;CAPE對(duì)人結(jié)腸癌HT-29細(xì)胞增殖凋亡及NF-κB信號(hào)通路影響的實(shí)驗(yàn)研究[D];南華大學(xué);2011年

，

本文編號(hào)：2122668