【摘要】：目的探討上皮-間質(zhì)轉(zhuǎn)化(EMT)調(diào)控蛋白在前列腺癌(PCa)侵襲、轉(zhuǎn)移中的作用及其對(duì)臨床預(yù)后的診斷價(jià)值。方法收集48例PCa組織樣本(PCa組)和50例良性前列腺增生(BPH)組織樣本(BPH組),免疫組化SP法檢測(cè)樣本神經(jīng)鈣黏素(N-cadherin)、上皮鈣黏素(E-cadherin)表達(dá),分析N-cadherin、E-cadherin表達(dá)與PCa患者臨床資料的關(guān)系。對(duì)PCa患者進(jìn)行隨訪,記錄患者總生存期(OS);以O(shè)S作為評(píng)價(jià)指標(biāo),采用單變量和多變量Cox比例風(fēng)險(xiǎn)模型評(píng)價(jià)患者預(yù)后的影響因素。以轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)誘導(dǎo)DU-145細(xì)胞發(fā)生EMT,細(xì)胞增殖實(shí)驗(yàn)檢測(cè)細(xì)胞增殖能力,Transwell實(shí)驗(yàn)檢測(cè)細(xì)胞遷移能力,Western blot檢測(cè)細(xì)胞E-cadherin、N-cadherin蛋白表達(dá)。結(jié)果 PCa組N-cadherin表達(dá)水平顯著高于BPH組,E-cadherin表達(dá)水平顯著低于BPH組(均P0.05)。腫瘤直徑≥2.5cm者N-cadherin高表達(dá)率顯著高于腫瘤直徑2.5cm者,Ⅳ期PCa患者N-cadherin高表達(dá)率顯著高于Ⅱ期和Ⅲ期PCa患者,淋巴結(jié)轉(zhuǎn)移者N-cadherin高表達(dá)率顯著高于無(wú)淋巴結(jié)轉(zhuǎn)移者,遠(yuǎn)處轉(zhuǎn)移者N-cadherin高表達(dá)率顯著高于無(wú)遠(yuǎn)處轉(zhuǎn)移者(均P0.05)。Gleason分級(jí)≥7分者E-cadherin高表達(dá)率顯著低于Gleason分級(jí)≤6分者,有淋巴結(jié)轉(zhuǎn)移者E-cadherin高表達(dá)率顯著低于無(wú)淋巴結(jié)轉(zhuǎn)移者,有遠(yuǎn)處轉(zhuǎn)移者E-cadherin高表達(dá)率顯著低于無(wú)遠(yuǎn)處轉(zhuǎn)移者(均P0.05)。N-cadherin高表達(dá)患者OS顯著低于N-cadherin低表達(dá)患者(P=0.024),E-cadherin高表達(dá)患者OS顯著高于E-cadherin低表達(dá)患者(P=0.017)。單因素和多因素分析顯示,T4期腫瘤、N-cadherin高表達(dá)、E-cadherin低表達(dá)是影響患者OS的獨(dú)立危險(xiǎn)因素(均P0.05)。細(xì)胞增殖實(shí)驗(yàn)顯示,第24~96h,DU-145組吸光度值顯著高于NC組(均P0.05);Transwell實(shí)驗(yàn)顯示,DU-145組穿膜細(xì)胞數(shù)量顯著多于NC組(P0.05);Western blot實(shí)驗(yàn)顯示,DU-145組N-cadherin蛋白表達(dá)水平顯著高于NC組,E-cadherin蛋白表達(dá)水平顯著低于NC組(均P0.05)。結(jié)論 EMT與PCa的增殖、侵襲和遷移有關(guān),N-cadherin、E-cadherin有可能作為PCa臨床預(yù)后的診斷指標(biāo)之一。
[Abstract]:Objective to investigate the role of EMT regulatory protein in the invasion and metastasis of prostate cancer (PCA) and its clinical prognostic value. Methods the expression of N-cadherin (N-cadherin) and E-cadherin (E-cadherin) in 48 patients with PCA and 50 patients with benign prostatic hyperplasia (BPH) were detected by immunohistochemical SP method. The relationship between the expression of N-cadherin and the clinical data of PCA was analyzed. Patients with PCA were followed up to record the total survival time (OS), and the single and multivariate Cox proportional risk models were used to evaluate the prognostic factors. Transforming growth factor 尾 1 (TGF- 尾 1) was used to induce EMTs in DU-145 cells. Cell proliferation assay and transwell assay were used to detect cell migration ability. Western blot was used to detect E-cadherin N-cadherin protein expression in DU-145 cells. Results the expression of N-cadherin in PCA group was significantly higher than that in BPH group (P0.05). The high expression rate of N-cadherin in patients with tumor diameter 鈮，

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