本文選題：microRNA + 34a　； 參考：《吉林大學》2017年碩士論文

【摘要】：目的:通過檢測牙齦鱗狀細胞癌(gingival squamous cell carcinoma,GSCC)及癌旁非瘤組織中小分子RNA 34a(micro RNA 34a,miR-34a)的表達水平,探討miR-34a與GSCC發(fā)病部位、大小、惡性程度以及患者的年齡和性別等臨床病理特征之間的關(guān)系。方法:在患者知情同意下,收集2014-2016年吉林大學口腔醫(yī)學院頜面外科30例確診為GSCC患者的手術(shù)切除腫瘤及相應癌旁非瘤組織。采用qRT-PCR法檢測GSCC及癌旁非瘤組織中miR-34a的表達水平。結(jié)果:與癌旁非瘤組織相比,GSCC組織中miR-34a的表達水平明顯下降(0.47±0.26,P=0.004);GSCC患者腫瘤組織中miR-34a的表達水平與年齡、性別、所在部位及有無淋巴結(jié)轉(zhuǎn)移等無關(guān)聯(lián)(P0.05),但與腫瘤組織的大小及惡性程度有關(guān)聯(lián)(P0.05)。結(jié)論:1.通過qRT-PCR技術(shù)檢測,miR-34a在GSCC患者腫瘤組織中的表達較癌旁非瘤組織明顯下調(diào),提示可能與GSCC的發(fā)生、發(fā)展密切相關(guān)。2.結(jié)合臨床病理資料,在GSCC患者的腫瘤組織中,miR-34a相對表達水平降低,且與腫瘤體積,分化程度關(guān)系密切(P0.05),提示可進一步作為臨床上GSCC的重要診斷指標,并對患者術(shù)后腫瘤復發(fā)、死亡等預后作出更準確預測,為進一步個體化,精準的癌癥治療提供依據(jù)。
[Abstract]:Objective: to detect the expression of small molecule RNA34a (micro RNA34a) in (gingival squamous cell carcinomas of gingival squamous cell carcinoma (GSCC) and its adjacent non-tumor tissues, and to investigate the location and size of miR-34a and GSCC. The relationship between malignancy and clinicopathological features such as age and sex. Methods: 30 patients with GSCC diagnosed as GSCC from 2014 to 2016 were collected with informed consent from Jilin University School of Stomatology. The expression of miR-34a in GSCC and noncancerous tissues was detected by qRT-PCR. Results: the expression level of miR-34a in GSCC was significantly decreased (0.47 鹵0.26 P0. 004) compared with that in non-cancerous tissues adjacent to cancer. The expression level of miR-34a in GSCC patients was significantly lower than that in patients with GSCC, and the expression level of miR-34a in GSCC was significantly lower than that in patients with GSCC. There was no correlation between the location and lymph node metastasis (P0.05), but there was a correlation with the size of tumor tissue and the degree of malignancy (P0.05). Conclusion 1. The expression of miR-34a in GSCC patients was down-regulated by qRT-PCR, which suggested that it might be closely related to the occurrence and development of GSCC. In combination with clinicopathological data, the relative expression of miR-34a was decreased in GSCC patients, and was closely related to tumor volume and differentiation (P0.05), which suggested that it could be further used as an important diagnostic index for GSCC and the recurrence of GSCC after operation. The prognosis of death is predicted more accurately, which provides the basis for further individualized and accurate cancer treatment.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R739.8

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