發(fā)布時(shí)間：2018-07-06 14:41

  本文選題：HCCR + Survivin��； 參考：《甘肅中醫(yī)藥大學(xué)(原名：甘肅中醫(yī)學(xué)院)》2015年碩士論文

【摘要】：背景:胃癌(gastric cancer)是世界上最為常見的惡性腫瘤之一,胃癌(gastric cancer)的發(fā)生及發(fā)展是一個(gè)多階段、多步驟、有序的過程,是多因素的綜合結(jié)果,其中抑癌基因、癌基因及一些生長因子在其中發(fā)揮了重要的作用。目的:觀察人宮頸癌基因(HCCR)、凋亡抑制蛋白(Survivin)、PTEN在胃癌組織、癌旁組織、正常胃組織中的表達(dá)特點(diǎn),并探究它們與胃癌臨床病理特征之間的關(guān)系。檢測PTEN、Survivin和HCCR的表達(dá)及之間的關(guān)系,探討HCCR、Survivin和PTEN在胃癌發(fā)生、發(fā)展、預(yù)后中的作用機(jī)理,探尋胃癌發(fā)生、發(fā)展的分子機(jī)理,了解其癌變的信號(hào)傳導(dǎo)通路,并為胃癌的分子靶向治療提供探索實(shí)驗(yàn)及臨床治療依據(jù)。方法:1、收集2012年11月-2013年11月期間甘肅省人民醫(yī)院手術(shù)和內(nèi)窺鏡結(jié)合病理確診的正常胃組織60例、癌旁組織60例、胃癌組織60例標(biāo)本并經(jīng)病理確診。2、所選標(biāo)本的患者在手術(shù)前均未接受放療、化療和各種針對(duì)性診治,無并發(fā)其它腫瘤。3、應(yīng)用免疫組化S-P法檢測60例胃癌組織、60例癌旁組織、60例正常胃黏膜組織中HCCR和Survivin、PTEN基因蛋白的表達(dá)。所有實(shí)驗(yàn)數(shù)據(jù)均采用SPSS22.0統(tǒng)計(jì)軟件包進(jìn)行處理,統(tǒng)計(jì)學(xué)處理使用卡方檢驗(yàn)和Spearman相關(guān)性分析,以P0.05為差異有統(tǒng)計(jì)學(xué)意義。結(jié)果:HCCR在60例正常胃組織中僅有2例陽性表達(dá),陽性表達(dá)率為3.3%,在60例癌旁組織中27例呈陽性表達(dá),其陽性表達(dá)率為45%;高于正常胃組織,在60例胃癌組織中43例為陽性表達(dá),陽性表達(dá)率為71.7%;呈高表達(dá),從正常胃組織、癌旁組織到胃癌組織,HCCR的陽性表達(dá)率呈逐漸遞增趨勢;在癌旁組織和胃癌組織中的表達(dá)明顯高于正常組織,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);在癌旁組織中的表達(dá)和胃癌組織中表達(dá)也具有統(tǒng)計(jì)學(xué)意義(P0.05)。PTEN在60例正常胃組織中有58例呈陽性表達(dá),陽性表達(dá)率為96.7%,在60例癌旁組織中有47例呈陽性表達(dá),陽性表達(dá)率為78.3%,在60例胃癌組織中有28例呈陽性表達(dá),陽性表達(dá)率為46.7%。在正常胃組織、癌旁組織和胃癌組織中,PTEN的陽性表達(dá)率呈遞減趨勢,在癌旁組織和胃癌組織中明顯低于正常胃組織中的表達(dá),差異均具有統(tǒng)計(jì)學(xué)意義(P0.01)在癌旁組織和胃癌組織中的表達(dá)也具有統(tǒng)計(jì)學(xué)意義(P0.05)。Survivin在60例正常胃組織中有7例呈陽性表達(dá),陽性表達(dá)率為11.7%,在60例癌旁組織中有26例呈陽性表達(dá),陽性表達(dá)率為43.3%,在60例胃癌組織中有44例呈陽性表達(dá),陽性表達(dá)率為73.3%。在正常胃組織、癌旁組織和胃癌組織中,Survivin的陽性表達(dá)率呈明顯遞增趨勢,在癌旁組織和胃癌組織中明顯高于正常胃組織中的表達(dá),差異均具有統(tǒng)計(jì)學(xué)意義(P0.01)在癌旁組織和胃癌組織中的表達(dá)也具有統(tǒng)計(jì)學(xué)意義(P0.05)。HCCR和Survivin基因蛋白在胃癌組織中表達(dá)明顯,PTEN蛋白在胃癌組織中低表達(dá),三者差異有統(tǒng)計(jì)學(xué)意義(P0.05);Survivin基因蛋白表達(dá)與胃癌組織病理分級(jí)有關(guān)(P0.05)。結(jié)論:HCCR和Survivin基因蛋白在胃癌組織中均為高表達(dá),為胃癌早期診斷提供了新的生物學(xué)指標(biāo),同時(shí)在胃癌的治療方面,提出了新的治療策略。PTEN基因在正常胃組織、癌旁組織、胃癌組織中表達(dá)呈遞減趨勢,表明PTEN基因與胃癌的發(fā)生密切相關(guān),表明PTEN基因的表達(dá)可能成為臨床評(píng)價(jià)胃癌惡性程度及其預(yù)后提供新的指標(biāo)。
[Abstract]:Background: gastric cancer (gastric cancer) is one of the most common malignant tumors in the world. The occurrence and development of gastric cancer (gastric cancer) is a multistage, multistep and orderly process, which is a comprehensive result of multiple factors. The tumor suppressor gene, oncogene and some growth factors have played an important role in it. Objective: To observe the human cervical cancer base HCCR, apoptosis inhibitory protein (Survivin), PTEN expression in gastric cancer tissue, para cancer tissue and normal gastric tissue, and explore the relationship between them and the clinicopathological features of gastric cancer. The relationship between the expression of PTEN, Survivin and HCCR and the relationship between them are detected, and the mechanism of HCCR, Survivin and PTEN in the occurrence, development and prognosis of gastric cancer is explored. The molecular mechanism of the occurrence and development of gastric cancer, to understand the signal transduction pathway of its carcinogenesis, and to provide experimental and clinical basis for the molecular targeting therapy of gastric cancer. Methods: 1, 60 cases of normal gastric tissue, 60 cases of para cancer tissue, and gastric cancer group in Gansu people's hospital in November 2012, November, were collected by surgery and endoscopy combined with pathology. 60 specimens were weave and pathologically confirmed.2. The patients selected were not treated with radiotherapy, chemotherapy and various specific diagnosis and treatment and no other tumor.3 before operation. The immunohistochemical S-P method was used to detect 60 cases of gastric cancer tissue, 60 cases of para cancer tissue, and 60 normal gastric mucosa tissues, and the expression of HCCR and Survivin, PTEN gene protein. All of them were treated with SPSS22.0 software package. Statistical processing using chi square test and Spearman correlation analysis were statistically significant. Results: there were only 2 positive expressions in 60 normal gastric tissues in 60 cases, the positive expression rate was 3.3%, and 27 cases were positive in 60 para cancerous tissues, and the positive expression rate was 45%. The positive expression rate of 43 cases of normal gastric tissue was positive in 60 cases of gastric cancer, the positive expression rate was 71.7%, and the positive expression rate was gradually increasing from normal gastric tissue, para cancer tissue to gastric cancer tissue. The expression of HCCR was higher than normal tissue, and the difference was statistically significant (P0.05) in the para cancer tissue and gastric cancer tissue. Expression in tissue and expression in gastric cancer tissue were also statistically significant (P0.05).PTEN positive expression in 60 normal gastric tissues, positive expression rate was 96.7%, in 60 cases of para cancer tissues, 47 cases were positive, positive expression rate was 78.3%, 28 cases in 60 cases of gastric cancer were positive expression, the positive expression rate was 46.7%.. The positive expression rate of PTEN was decreasing in normal gastric tissue, para cancer tissue and gastric cancer tissue. The expression in para and gastric tissues was significantly lower than that in normal gastric tissue. The difference was statistically significant (P0.01) in the para cancer tissue and gastric cancer tissue (P0.05).Survivin in 60 normal gastric groups The positive expression rate of 7 cases was 11.7%. In 60 cases of para cancerous tissues, 26 cases were positive, the positive expression rate was 43.3%. In 60 cases of gastric cancer, 44 cases were positive, the positive expression rate was 73.3%. in normal gastric tissue, the positive expression rate of Survivin in the para cancer tissue and gastric cancer tissue was obviously increasing trend, in cancer. The expression of para tissue and gastric carcinoma was significantly higher than that in normal gastric tissue. The difference was statistically significant (P0.01) in the para cancer tissue and gastric cancer tissue (P0.05), the expression of.HCCR and Survivin gene protein in gastric cancer tissues was obvious, and the PTEN egg white was low in the gastric cancer tissue, and the difference between the three was statistically significant. Significance (P0.05); the expression of Survivin gene protein is related to the histopathological classification of gastric cancer (P0.05). Conclusion: the expression of HCCR and Survivin gene protein in gastric cancer tissues is high, which provides a new biological index for the early diagnosis of gastric cancer. At the same time, a new therapeutic strategy,.PTEN gene in normal gastric tissue and para cancer group, is proposed in the treatment of gastric cancer. The expression of the gastric cancer tissue is decreasing, which indicates that the PTEN gene is closely related to the occurrence of gastric cancer. It shows that the expression of PTEN gene may be a new index for the evaluation of the malignant degree and prognosis of gastric cancer.
【學(xué)位授予單位】：甘肅中醫(yī)藥大學(xué)(原名：甘肅中醫(yī)學(xué)院)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R735.2

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本文編號(hào)：2103126