本文選題：HBV-X基因 + 點突變　； 參考：《重慶醫(yī)科大學》2017年碩士論文

【摘要】：目的:探討乙肝病毒X區(qū)基因變異情況與慢性HBV感染患者血清中TGF-β1表達致肝細胞肝癌間的相關性。方法:收集2015年1月至2016年10月期間重慶醫(yī)科大學附屬第二醫(yī)院的慢性HBV患者血清,總共105例,其中包括肝炎組35例,肝硬化組35例,肝癌組35例,同時收集患者的基本資料、兩對半定量等實驗室檢查結果。采用聚合酶鏈反應(PCR)方法擴增HBV-X基因序列,并對PCR產(chǎn)物進行DNA測序,將測序結果與已知HBV-X基因序列進行對比分析,得出變異位點。酶聯(lián)免疫吸附測定法(ELISA)檢測患者血清中TGF-β1濃度。將所得的結果進行卡方檢驗、單因素方差分析及相關性分析等方法處理數(shù)據(jù)。結果:TGF-β1濃度在肝癌組表達明顯高于非肝癌組(34.4±29.9 vs11.4±16.8和10.1±12.2,P0.05),差異具有統(tǒng)計學意義。肝病患者血清中常見的突變位點依次為:A1762T/G1764A、T1719C、G1635A、A1605G、C1653T、T1753G,其中A1605G、A1762T/G1764A、T1753G突變率在肝癌組明顯高于非肝癌組,差異具有統(tǒng)計學意義(P0.05)。A1605G、T1719C位點突變與血清中TGF-β1濃度有相關性(r=0.513,P0.01、r=0.277,P0.01)。進一步分析發(fā)現(xiàn),在肝癌組中僅A1605G位點突變與TGF-β1濃度呈正相關(r=0.518,P0.05)。結論:HBV-X基因中的A1605G位點突變與TGF-β1表達呈正相關,這可能參與了HCC的發(fā)生發(fā)展進程。
[Abstract]:Objective: to investigate the relationship between the mutation of hepatitis B virus X region gene and the expression of TGF- 尾 1 in serum of patients with chronic HBV infection. Methods: from January 2015 to October 2016, 105 patients with chronic HBV were collected from the second affiliated Hospital of Chongqing Medical University, including 35 patients with hepatitis, 35 patients with liver cirrhosis and 35 patients with liver cancer. Two pairs of semi-quantitative laboratory results. The HBV-X gene sequence was amplified by polymerase chain reaction (PCR), and the DNA sequence of the PCR product was sequenced. The results were compared with the known HBV-X gene sequence, and the mutation sites were obtained. The concentration of TGF- 尾 1 in serum was detected by enzyme-linked immunosorbent assay (Elisa). The results were processed by chi-square test, single factor analysis of variance and correlation analysis. Results the expression of TGF- 尾 1 in HCC group was significantly higher than that in non-HCC group (34.4 鹵29.9 vs11.4 鹵16.8,10.1 鹵12.2 P 0.05), and the difference was statistically significant. The common mutation sites in the serum of liver disease patients were as follows: 1. A1762T / G1764AnT1719CG1635AA1605GC1653T1653T1753G, in which the mutation rate of A1605GN A1762T / G1764AN T1753G was significantly higher in HCC group than in non-HCC group, the difference was statistically significant (P0.05) .A1605GT1719C mutation was correlated with serum TGF- 尾 _ 1 concentration (r0.513P 0.01r277P0.01). The results showed that the mutation of T1753G in liver cancer group was significantly higher than that in non-hepatoma group (P0.05). There was a significant correlation between TGF- 尾 _ 1 mutation and serum TGF- 尾 _ 1 concentration (r 0.513 P 0.01r 277P0.01). Further analysis showed that only A1605G mutation was positively correlated with TGF- 尾 1 concentration in HCC group (r = 0.518, P 0.05). Conclusion the A1605G mutation in the 1: HBV-X gene is positively correlated with the expression of TGF- 尾 1, which may be involved in the development of HCC.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R735.7

【參考文獻】

相關期刊論文 前8條

1 Ming Geng;Xuan Xin;Li-Quan Bi;Lu-Ting Zhou;Xiao-Hong Liu;;Molecular mechanism of hepatitis B virus X protein function in hepatocarcinogenesis[J];World Journal of Gastroenterology;2015年38期

2 彭小華;壟堂英;楊求真;董麗清;;恩替卡韋與拉米夫定對乙型肝炎肝硬化患者血清TGF-β1的影響研究[J];中華醫(yī)院感染學雜志;2015年04期

3 楊威;楊紅杰;莫瑞祥;李西融;廖文勝;張慧明;;TGF-β1通過促進自噬相關基因Beclin 1表達抑制肝癌細胞增殖[J];華夏醫(yī)學;2014年06期

4 吳德海;邰升;;HBX促進肝細胞癌發(fā)生發(fā)展的分子機制[J];世界華人消化雜志;2014年25期

5 朱宏斌;楊云生;郭明洲;;肝癌中轉(zhuǎn)化生長因子(TGF)-β信號轉(zhuǎn)導通路的研究進展[J];胃腸病學和肝病學雜志;2012年03期

6 王恒毅;梁慧芳;陳孝平;劉偉鵬;張占國;于宗平;李常海;;乙型肝炎病毒編碼X蛋白降低大鼠肝臟卵圓細胞對β型轉(zhuǎn)化生長因子1增殖抑制效應敏感度的研究[J];中華外科雜志;2011年05期

7 朱杰;杜文波;高潤平;張瑞娟;張秀軍;;慢性乙型肝病患者肝組織和血清TGF-β1的測定及臨床意義[J];中國實驗診斷學;2010年07期

8 ;Hepatitis B virus X protein promotes proliferation and upregulates TGF-β1 and CTGF in human hepatic stellate cell line,LX-2[J];Hepatobiliary & Pancreatic Diseases International;2009年01期

相關博士學位論文 前1條

1 母小新;TGF-β信號通路參與肝癌發(fā)生發(fā)展的機制研究[D];南京醫(yī)科大學;2013年

相關碩士學位論文 前1條

1 陸海生;JNK信號通路介導HBx對RXRα的磷酸化并促進HCC進程的分子機制[D];廈門大學;2014年

，

本文編號：2089921