本文選題：HBV-X基因 + 點(diǎn)突變　； 參考：《重慶醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:探討乙肝病毒X區(qū)基因變異情況與慢性HBV感染患者血清中TGF-β1表達(dá)致肝細(xì)胞肝癌間的相關(guān)性。方法:收集2015年1月至2016年10月期間重慶醫(yī)科大學(xué)附屬第二醫(yī)院的慢性HBV患者血清,總共105例,其中包括肝炎組35例,肝硬化組35例,肝癌組35例,同時(shí)收集患者的基本資料、兩對(duì)半定量等實(shí)驗(yàn)室檢查結(jié)果。采用聚合酶鏈反應(yīng)(PCR)方法擴(kuò)增HBV-X基因序列,并對(duì)PCR產(chǎn)物進(jìn)行DNA測(cè)序,將測(cè)序結(jié)果與已知HBV-X基因序列進(jìn)行對(duì)比分析,得出變異位點(diǎn)。酶聯(lián)免疫吸附測(cè)定法(ELISA)檢測(cè)患者血清中TGF-β1濃度。將所得的結(jié)果進(jìn)行卡方檢驗(yàn)、單因素方差分析及相關(guān)性分析等方法處理數(shù)據(jù)。結(jié)果:TGF-β1濃度在肝癌組表達(dá)明顯高于非肝癌組(34.4±29.9 vs11.4±16.8和10.1±12.2,P0.05),差異具有統(tǒng)計(jì)學(xué)意義。肝病患者血清中常見(jiàn)的突變位點(diǎn)依次為:A1762T/G1764A、T1719C、G1635A、A1605G、C1653T、T1753G,其中A1605G、A1762T/G1764A、T1753G突變率在肝癌組明顯高于非肝癌組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。A1605G、T1719C位點(diǎn)突變與血清中TGF-β1濃度有相關(guān)性(r=0.513,P0.01、r=0.277,P0.01)。進(jìn)一步分析發(fā)現(xiàn),在肝癌組中僅A1605G位點(diǎn)突變與TGF-β1濃度呈正相關(guān)(r=0.518,P0.05)。結(jié)論:HBV-X基因中的A1605G位點(diǎn)突變與TGF-β1表達(dá)呈正相關(guān),這可能參與了HCC的發(fā)生發(fā)展進(jìn)程。
[Abstract]:Objective: to investigate the relationship between the mutation of hepatitis B virus X region gene and the expression of TGF- 尾 1 in serum of patients with chronic HBV infection. Methods: from January 2015 to October 2016, 105 patients with chronic HBV were collected from the second affiliated Hospital of Chongqing Medical University, including 35 patients with hepatitis, 35 patients with liver cirrhosis and 35 patients with liver cancer. Two pairs of semi-quantitative laboratory results. The HBV-X gene sequence was amplified by polymerase chain reaction (PCR), and the DNA sequence of the PCR product was sequenced. The results were compared with the known HBV-X gene sequence, and the mutation sites were obtained. The concentration of TGF- 尾 1 in serum was detected by enzyme-linked immunosorbent assay (Elisa). The results were processed by chi-square test, single factor analysis of variance and correlation analysis. Results the expression of TGF- 尾 1 in HCC group was significantly higher than that in non-HCC group (34.4 鹵29.9 vs11.4 鹵16.8,10.1 鹵12.2 P 0.05), and the difference was statistically significant. The common mutation sites in the serum of liver disease patients were as follows: 1. A1762T / G1764AnT1719CG1635AA1605GC1653T1653T1753G, in which the mutation rate of A1605GN A1762T / G1764AN T1753G was significantly higher in HCC group than in non-HCC group, the difference was statistically significant (P0.05) .A1605GT1719C mutation was correlated with serum TGF- 尾 _ 1 concentration (r0.513P 0.01r277P0.01). The results showed that the mutation of T1753G in liver cancer group was significantly higher than that in non-hepatoma group (P0.05). There was a significant correlation between TGF- 尾 _ 1 mutation and serum TGF- 尾 _ 1 concentration (r 0.513 P 0.01r 277P0.01). Further analysis showed that only A1605G mutation was positively correlated with TGF- 尾 1 concentration in HCC group (r = 0.518, P 0.05). Conclusion the A1605G mutation in the 1: HBV-X gene is positively correlated with the expression of TGF- 尾 1, which may be involved in the development of HCC.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.7

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本文編號(hào)：2089921