本文選題：急性淋巴細(xì)胞白血病 + 成纖維細(xì)胞生長(zhǎng)因子受體��； 參考：《中國(guó)病理生理雜志》2017年04期

【摘要】：目的:了解急性淋巴細(xì)胞白血病(ALL)患者成纖維細(xì)胞生長(zhǎng)因子受體3(FGFR3)表達(dá)對(duì)循環(huán)內(nèi)皮細(xì)胞(CECs)增殖的影響。方法:通過(guò)RT-PCR法檢測(cè)44例ALL患者骨髓中FGFR3 mRNA表達(dá),分成FGFR3~+組及FGFR3~-組進(jìn)行Kaplan-Meier生存分析。利用免疫磁珠結(jié)合流式細(xì)胞術(shù)分離計(jì)數(shù)CECs,對(duì)比兩組CECs數(shù)量、表面抗原表達(dá)、生長(zhǎng)曲線及集落生成數(shù)的差異。免疫熒光組化染色測(cè)定CECs表面FGFR3表達(dá)水平。結(jié)果:44例核型正常ALL患者中FGFR3 mRNA表達(dá)陽(yáng)性率為43.2%,T-ALL組的FGFR3表達(dá)高于B-ALL組(P0.05)。19 d骨髓原始細(xì)胞比例≥5%的患者FGFR3表達(dá)升高(P0.05)。FGFR3陽(yáng)性組的總生存率明顯低于陰性組(P0.05)。分離出的CECs高表達(dá)CD31、CD144、VEGFR-2和CD146,基本不表達(dá)CD45。與FGFR3陰性組相比,陽(yáng)性組CECs數(shù)量、CD133的陽(yáng)性率及集落生成數(shù)均增多(P0.05)。體外培養(yǎng)中,FGFR3陽(yáng)性組與陰性組相比,生長(zhǎng)曲線3個(gè)時(shí)點(diǎn)上的細(xì)胞數(shù)差異有統(tǒng)計(jì)學(xué)顯著性(P0.05)。19例ALL-FGFR3~+患者CECs表面均能檢測(cè)到FGFR3表達(dá),陽(yáng)性率為29.00%±15.71%。結(jié)論:致癌基因FGFR3對(duì)ALL患者CECs的增殖有促進(jìn)作用,可能具備了抗腫瘤及抗血管生成的雙重靶點(diǎn)身份,可以為今后的分子治療提供更多的選擇。
[Abstract]:Objective: to understand the effect of fibroblast growth factor receptor 3 (FGFR3) expression on the proliferation of circulating endothelial cells (CECs) in patients with acute lymphoblastic leukemia (ALL). Methods: the expression of FGFR3 mRNA in bone marrow of 44 patients with ALL was detected by RT-PCR, and the Kaplan-Meier survival analysis was divided into FGFR3~+ and FGFR3~- groups. The immunomagnetic bead binding flow formula was used. The number of CECs, the expression of surface antigen, the growth curve and the number of colony formation were compared between the two groups of CECs. The expression of FGFR3 expression on the surface of CECs was measured by immunohistochemical staining. Results: the positive rate of FGFR3 mRNA expression was 43.2% in 44 cases of normal ALL patients, and the FGFR3 expression in T-ALL group was higher than that of B-ALL group (P0.05).19 marrow primitive fine bone marrow The total survival rate of FGFR3 expression increased (P0.05).FGFR3 positive group was significantly lower than that of negative group (P0.05). The CECs high expression of CECs expressed CD31, CD144, VEGFR-2 and CD146. The number of CECs in the positive group, the positive rate and the colony formation number in the positive group were increased. Compared with the negative group, there was a significant difference in the number of cells at the 3 time points of the growth curve (P0.05). The expression of FGFR3 was detected on the surface of CECs in.19 cases of ALL-FGFR3~+ patients, and the positive rate was 29% + 15.71%. conclusion: the carcinogenic gene FGFR3 could promote the proliferation of CECs in ALL patients, and may have dual anti tumor and anti angiogenesis. Target status can provide more choices for molecular therapy in the future.
【作者單位】： 寧波大學(xué)醫(yī)學(xué)院附屬鄞州醫(yī)院血液科;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院同濟(jì)醫(yī)院血液科;
【基金】：浙江省自然科學(xué)基金資助項(xiàng)目(No.LQ12H08001) 浙江省衛(wèi)生廳一般研究項(xiàng)目資助(No.201235256) 寧波市自然科學(xué)基金資助項(xiàng)目(No.2012A610236) 寧波市醫(yī)學(xué)科技計(jì)劃資助項(xiàng)目(No.2011B23)
【分類號(hào)】：R733.71

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