發(fā)布時(shí)間：2018-06-23 21:55

  本文選題：非小細(xì)胞肺癌 + 甲狀腺轉(zhuǎn)錄因子-1��； 參考：《皖南醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:本研究通過檢測(cè)非小細(xì)胞肺癌(non-small cell lung cancer,NSCLC)中甲狀腺轉(zhuǎn)錄因子-1(Thyroid Transcription Factor-1,TTF-1)和Ki-67(細(xì)胞增殖核抗原)的表達(dá),并統(tǒng)計(jì)它們各自與患者各臨床指標(biāo)的關(guān)系,然后通過統(tǒng)計(jì)軟件檢測(cè)兩者之間的關(guān)系,以便為認(rèn)識(shí)和了解NSCLC的特性提供一定的幫助。方法:選取皖南醫(yī)學(xué)院弋磯山醫(yī)院2015.1-2016.6住院在呼吸內(nèi)科和胸心外科的非小細(xì)胞肺癌患者(組織標(biāo)本來源途徑分別為支氣管鏡活檢標(biāo)本,CT引導(dǎo)下穿刺活檢標(biāo)本和外科手術(shù)切除標(biāo)本,確診方法為病理學(xué)檢查),例數(shù)為130例。通過免疫組化(Immunohistochemistry,IHC)的方法測(cè)定這些患者組織標(biāo)本中的TTF-1和Ki-67表達(dá)情況,來探討兩者各自與患者臨床特征的關(guān)系,并測(cè)定TTF-1和Ki-67兩者的關(guān)系。統(tǒng)計(jì)軟件用SPSS17.0。統(tǒng)計(jì)TTF-1和Ki-67的與各參數(shù)的關(guān)系時(shí)用卡方檢驗(yàn)、統(tǒng)計(jì)TTF-1和Ki-67的相關(guān)性時(shí)用秩相關(guān)檢驗(yàn)。結(jié)果:1.TTF-1在腺癌、女性、中高分化、I-II期的NSCLC患者中陽性率高,而與NSCLC患者的年齡、淋巴結(jié)轉(zhuǎn)移情況、有無吸煙等無關(guān)。2.Ki-67在有吸煙史、低分化、有淋巴結(jié)轉(zhuǎn)移的NSCLC患者中陽性率高,而與病理類型、性別、年齡、分期無關(guān)。3.在NSCLC中,TTF-1和Ki-67兩者的表達(dá)無明顯相關(guān)性(r=0.173,p0.05)。結(jié)論:1、TTT-1對(duì)于診斷肺腺癌有較積極的意義。2、在NSCLC患者當(dāng)中女性、分化程度越高,臨床分期越早,甲狀腺轉(zhuǎn)錄因子-1表達(dá)陽性率越高。TTF-1可能參與NSCLC的侵襲,可能在NSCLC侵襲通路中的某一環(huán)節(jié)扮演著重要作用。3、在NSCLC患者當(dāng)中吸煙患者、分化程度越低差、淋巴結(jié)轉(zhuǎn)移患者,Ki-67表達(dá)陽性率越高。Ki-67可能參與NSCLC的浸潤、轉(zhuǎn)移,可能在NSCLC的浸潤和轉(zhuǎn)移通路中的某一環(huán)節(jié)扮演著重要作用。
[Abstract]:Objective: to investigate the expression of Thyroid transcription-Factor-1- TTF-1 and Ki-67 in non-small cell lung cancerous NSCLC, and to investigate the relationship between them and the clinical parameters of the patients, and to investigate the relationship between the expression of TTF-1 and Ki-67 (proliferative nuclear antigen) in patients with non-small cell lung cancer (NSCLC), and to investigate the relationship between the expression of TTF-1 and Ki-67 in patients with non-small cell lung cancer. Then the relationship between NSCLC and NSCLC is detected by statistical software in order to provide some help for understanding and understanding the characteristics of NSCLC. Methods: selected non-small cell lung cancer (NSCLC) patients hospitalized in Department of Respiratory Medicine and Thoracic surgery from January to June 2015.The source of tissue samples were bronchoscopic biopsy specimens guided by CT and surgical excision specimens. The method of diagnosis was pathological examination (130 cases). The expression of TTF-1 and Ki-67 in these patients was determined by immunohistochemical method to explore the relationship between them and clinical features, and the relationship between TTF-1 and Ki-67. SPSS 17.0. The correlation between TTF-1 and Ki-67 and parameters was measured by chi-square test, and the correlation between TTF-1 and Ki-67 was measured by rank correlation test. Results 1. The positive rate of TTF-1 in NSCLC patients with adenocarcinoma, female and middle and high differentiation stage I-II was higher than that of NSCLC with age, lymph node metastasis, smoking or not. The positive rate of Ki-67 in NSCLC patients with smoking history, low differentiation and lymph node metastasis was higher than that in NSCLC patients with lymph node metastasis. But not related to pathological type, sex, age, stage. There was no significant correlation between the expression of TTF-1 and Ki-67 in NSCLC (r = 0.173, p0.05). Conclusion TTF-1 may play a positive role in the diagnosis of lung adenocarcinoma. In NSCLC patients, the higher the differentiation degree and the earlier the clinical stage, the higher the positive rate of TTF-1 expression. TTF-1 may be involved in the invasion of NSCLC. It may play an important role in the invasion pathway of NSCLC. In NSCLC patients, the lower the differentiation degree, the higher the positive rate of Ki-67 expression. Ki-67 may be involved in the invasion and metastasis of NSCLC. It may play an important role in the infiltration and metastasis of NSCLC.
【學(xué)位授予單位】：皖南醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R734.2

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