發(fā)布時間：2018-06-22 00:13

  本文選題：食管癌 + 氧化應(yīng)激�。� 參考：《川北醫(yī)學(xué)院》2017年碩士論文

【摘要】：研究背景:食管癌的診斷主要依據(jù)患者的臨床表現(xiàn)、影像檢查及病理活檢等,腫瘤標(biāo)志物在食管癌的診斷及判定預(yù)后中特異性較差。有研究報道,氧化應(yīng)激在多種腫瘤的發(fā)生發(fā)展中扮演了重要角色,而氧化應(yīng)激相關(guān)參數(shù),如總抗氧化狀態(tài)(total antioxidant status,TAS),總氧化狀態(tài)(total oxidant status,TOS)及氧化應(yīng)激指數(shù)(oxidative stress index,OSI)等在患者血液中的表達量較健康人群均有不同程度的升高或降低。上述三個參數(shù)聯(lián)合測定在乳腺癌及甲狀腺癌中已有報道,且其表達量與疾病預(yù)后及分期相關(guān),但在食管癌中聯(lián)合測定的文章報道較少。有文獻報道,在應(yīng)激狀態(tài)下,YB-1(Y-box-binding protein 1)蛋白的表達上調(diào),但目前尚無文獻報道氧化應(yīng)激參數(shù)與YB-1蛋白的關(guān)系。目的:本研究通過測定食管癌患者血清中相關(guān)氧化應(yīng)激參數(shù)與健康人群的表達水平、吸煙史、飲酒史、治療效果及臨床分期之間的關(guān)系,同時檢測胃鏡活檢的食管癌標(biāo)本中YB-1蛋白的表達水平與氧化應(yīng)激參數(shù)之間的關(guān)系,為食管癌的臨床診斷和判定預(yù)后提供理論依據(jù)。方法:隨機收集我院92例不同臨床分期及治療前的食管癌患者和64例健康體檢者的空腹血液,測定其血清中TOS及TAS含量,并計算出OSI值。比較食管癌患者與健康體檢者血清中氧化應(yīng)激參數(shù)的差異,同時還比較了氧化應(yīng)激參數(shù)在食管癌不同臨床分期、有無煙酒史、短期療效及與YB-1蛋白表達水平之間的差異。統(tǒng)計學(xué)處理采用IBM SPSS 22.0統(tǒng)計軟件進行統(tǒng)計分析,P0.05視為有統(tǒng)計學(xué)差異。結(jié)果:在食管癌患者中,TOS值較健康人群低,差異有統(tǒng)計學(xué)意義(P0.01),而食管癌患者的TAS值比健康人群明顯低,差異統(tǒng)計學(xué)意義(P0.01),兩組間OSI比值同樣有統(tǒng)計學(xué)意義(P0.01)。TOS、TAS及OSI值與患者YB-1蛋白表達水平、臨床分期、吸煙及飲酒史及短期療效間無統(tǒng)計學(xué)意義(P0.05)。結(jié)論:(1)食管癌患者血清中TAS及TOS可能是潛在的生物標(biāo)記物。(2)對ROC曲線分析發(fā)現(xiàn),TOS、TAS對食管癌的診斷具有一定參考價值。(3)病檢標(biāo)本測定的YB-1蛋白的表達水平與食管癌患者血清中TOS、TAS及OSI進行對比無相關(guān)性。
[Abstract]:Background: the diagnosis of esophageal carcinoma is mainly based on the clinical manifestations, imaging examination and pathological biopsy. The specificity of tumor markers in the diagnosis and prognosis of esophageal cancer is poor. It has been reported that oxidative stress plays an important role in the occurrence and development of many kinds of tumors, and the parameters related to oxidative stress, The expression of total antioxidant (total antioxidant status tas, total oxidized (total oxidant status TOS and oxidative stress index (oxidative stress index OSI in the blood of the patients were higher or lower than those of the healthy subjects. The combined determination of these three parameters has been reported in breast cancer and thyroid carcinoma, and the expression of these parameters is related to the prognosis and stage of the disease, but there are few reports of combined determination in esophageal carcinoma. It has been reported that the expression of YB-1 (Y-box-binding protein 1) protein is up-regulated under stress, but the relationship between oxidative stress parameters and YB-1 protein has not been reported. Objective: to study the relationship between serum oxidative stress parameters and the expression level, smoking history, drinking history, therapeutic effect and clinical stage of patients with esophageal cancer. At the same time, the relationship between the expression of YB-1 protein and the parameters of oxidative stress was detected in the esophageal carcinoma specimens by gastroscopy biopsy, which provided a theoretical basis for the clinical diagnosis and prognosis of esophageal carcinoma. Methods: the fasting blood samples of 92 patients with esophageal cancer and 64 healthy controls were collected randomly. The serum TOS and TAS levels were measured and the OSI values were calculated. To compare the difference of serum oxidative stress parameters between esophageal cancer patients and healthy controls, and to compare the differences of oxidative stress parameters in different clinical stages of esophageal cancer, history of tobacco and alcohol, short-term efficacy and YB-1 protein expression level. Statistical analysis using IBM SPSS 22.0 statistical software was regarded as statistical difference (P0.05). Results: the values of TOS in patients with esophageal cancer were significantly lower than those in healthy subjects (P0.01), and the TAS values of patients with esophageal cancer were significantly lower than those of healthy subjects. There was no significant difference between the OSI ratio of the two groups (P0.01). TOSTAS and OSI values and YB-1 protein expression level, clinical stage, smoking and drinking history and short-term curative effect (P0.05). Conclusion: (1) TAS and TOS may be potential biomarkers in the serum of esophageal cancer patients. (2) the analysis of ROC curve shows that TOSTAS has some reference value in the diagnosis of esophageal carcinoma. (3) the expression level of YB-1 protein and the expression of YB-1 protein in the specimens of esophageal cancer and esophageal carcinoma are determined. There was no correlation between TOSTAS and OSI in serum.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R735.1

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本文編號：2050661