本文選題：bcl-基因 + 結(jié)直腸腫瘤　； 參考：《中國(guó)病理生理雜志》2017年05期

【摘要】：目的:研究bcl-3基因?qū)θ私Y(jié)腸癌RKO細(xì)胞遷移及凋亡的影響及機(jī)制。方法:采用人bcl-3基因的RNA干擾慢病毒載體沉默人結(jié)腸癌RKO細(xì)胞bcl-3基因的表達(dá)后,劃痕實(shí)驗(yàn)觀察bcl-3基因沉默前后RKO細(xì)胞遷移能力的變化,Annexin V/PI雙染色法檢測(cè)bcl-3基因沉默前后RKO細(xì)胞凋亡率的變化,Western blot法檢測(cè)bcl-3基因沉默前后細(xì)胞周期蛋白cyclin D1及凋亡相關(guān)蛋白Bax、Bcl-2的變化。結(jié)果:劃痕實(shí)驗(yàn)顯示,劃痕后36 h,bcl-3基因沉默前后RKO細(xì)胞劃痕愈合率分別為84.00%及40.00%,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。Annexin V/PI雙染色法流式細(xì)胞術(shù)分析顯示,bcl-3基因沉默前后的RKO細(xì)胞均經(jīng)5μmol/L順鉑處理24 h后,沉默前后的RKO細(xì)胞凋亡率分別為12.89%及59.67%,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。Western blot法檢測(cè)顯示bcl-3基因沉默后cyclin D1蛋白表達(dá)顯著下降(P0.05),Bax蛋白表達(dá)顯著上升(P0.05),但Bcl-2表達(dá)無(wú)明顯變化(P0.05)。結(jié)論:沉默bcl-3基因后,RKO細(xì)胞遷移能力下降,凋亡率增加,并伴細(xì)胞周期蛋白cyclin D1及凋亡相關(guān)蛋白Bax表達(dá)的變化。bcl-3基因可能通過(guò)改變cyclin D1及Bax蛋白的表達(dá)而影響RKO細(xì)胞的凋亡。
[Abstract]:Aim: to study the effect and mechanism of bcl-3 gene on migration and apoptosis of human colon cancer RKO cells. Methods: the expression of bcl-3 gene in human colon cancer RKO cells was silenced by human bcl-3 RNAi lentivirus vector. Effects of bcl-3 gene silencing on the migration of RKO cells and the apoptosis of RKO cells before and after bcl-3 gene silencing by Annexin V / Pi double staining and Western blot assay before and after bcl-3 gene silencing were used to detect the apoptosis of RKO cells and the apoptosis of RKO cells before and after bcl-3 gene silencing. Changes of related protein Baxfen-2. Results: scratch test showed that, The rate of scar healing of RKO cells was 84.00% and 40.005%, respectively, 36 h after the gene silencing. The difference was statistically significant (P 0.05) .Annexin V / Pi double staining flow cytometry analysis showed that the RKO cells were treated with 5 渭 mol / L cisplatin for 24 h before and after the silencing of Bcl-3 gene, and the results showed that RKO cells were treated with 5 渭 mol / L cisplatin for 24 h. The apoptosis rates of RKO cells before and after silencing were 12.89% and 59.67, respectively. The difference was statistically significant (P 0.05). Western blot assay showed that the expression of cyclin D1 protein decreased significantly after bcl-3 gene silencing, and the expression of cyclin D1 protein increased significantly (P 0.05%), but the expression of Bcl-2 did not change significantly (P0.05). Conclusion: after silencing bcl-3 gene, the migration ability of RKO cells decreased and the apoptosis rate increased. With the change of cyclin D1 and Bax expression, bcl-3 gene may affect the apoptosis of RKO cells by changing the expression of cyclin D1 and Bax proteins.
【作者單位】： 寧波大學(xué)醫(yī)學(xué)院附屬醫(yī)院腫瘤內(nèi)科;寧波市醫(yī)學(xué)科學(xué)研究所;
【基金】：寧波市社會(huì)發(fā)展科研項(xiàng)目(No.2011C50010)
【分類號(hào)】：R730.23

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本文編號(hào)：2030807