發(fā)布時間：2018-06-17 04:27

  本文選題：斯氏艾美球蟲 + 結(jié)腸癌��； 參考：《中國寄生蟲學(xué)與寄生蟲病雜志》2017年04期

【摘要】：目的探討斯氏艾美球蟲(Eimeria stiedai)可溶性蛋白(EsSP)對荷瘤小鼠腫瘤生長、生存率和免疫狀態(tài)的影響。方法建立小鼠皮下結(jié)腸癌(CT26)腫瘤模型,確定最小100%致瘤量腫瘤細(xì)胞數(shù)。取10~8個斯氏艾美球蟲孢子化卵囊,采用超聲波間斷乳化制備EsSP。105只雄性BALB/c小鼠按隨機(jī)數(shù)表法均分為7組(15只/組),每組小鼠于右側(cè)腋窩皮下接種CT26細(xì)胞5×10~5個,其中6個實驗組(A~F組)小鼠分別腹腔注射100.00、50.00、10.00、1.00、0.10、0.01μg/d EsSP,1次/d×5 d,對照組腹腔注射等量PBS。于接種后第7、11、13、15、17、19、21、23天測量腫瘤直徑,計算相對腫瘤體積和相對腫瘤增殖率(T/C)。接種后第25天每組各處死5只小鼠,稱量瘤重,計算抑瘤率。采眼球血,分離小鼠外周血淋巴細(xì)胞,MTS比色法檢測EsSP對荷瘤小鼠淋巴細(xì)胞增殖能力的影響,計算刺激指數(shù)(SI);流式細(xì)胞術(shù)檢測外周血CD4~+/CD8~+T淋巴細(xì)胞比值變化。記錄各組荷瘤小鼠死亡時間和死亡數(shù)量,共觀察80 d。各組間差異性比較采用單因素方差分析。結(jié)果最小100%致瘤量腫瘤細(xì)胞數(shù)為5×10~5個。接種結(jié)腸癌細(xì)胞后第23天,A、B、C組的腫瘤體積為(435.2±41.1)、(366.3±29.2)、(460.2±28.5)mm~3,較E、F和對照組的(761.2±33.2)、(810.4±38.4)、(865.2±35.3)mm~3生長緩慢(P0.05);A、B、C組的T/C分別為(39.0±6.7)%、(33.3±8.9)%、(35.0±8.1)%,均40%。B組小鼠瘤重為(1.109±0.432)g,低于對照組的(1.946±0.289)g(P0.05),抑瘤率最高,為(43.0±14.6)%。MTS比色法檢測結(jié)果顯示,B、C組小鼠SI分別為1.75±0.15、1.70±0.32,高于對照組的1.38±0.18(P0.05)。流式細(xì)胞術(shù)檢測結(jié)果顯示,A、B、C組小鼠外周血中CD4~+/CD8~+T淋巴細(xì)胞亞群的比值分別為1.58±0.24、1.74±0.22、1.61±0.16,與對照組的1.34±0.15比較,差異均有統(tǒng)計學(xué)意義(P0.01或P0.05)。荷瘤小鼠生存率結(jié)果顯示,至第80天,B、C組各存活5只小鼠,高于對照組的2只(P0.05)。結(jié)論 EsSP能夠抑制結(jié)腸癌皮下腫瘤的生長,提高荷瘤小鼠生存率,改變腫瘤誘導(dǎo)的免疫抑制狀態(tài),增強(qiáng)小鼠抗腫瘤免疫反應(yīng)。
[Abstract]:Objective to investigate the effects of Eimeria stiedaii (Eimeria stiedaii) soluble protein EsSPs on tumor growth, survival rate and immune status in tumor-bearing mice. Methods the tumor model of murine subcutaneous colon cancer (CT26) was established, and the tumor cell number of the tumor was determined at least 100%. The spore oocysts of Eimeria skrjabini were collected and 105 male BALB / c mice were prepared by ultrasonic discontinuous emulsification. According to the random number table, 105 male BALB / c mice were divided into 7 groups with 15 oocysts in each group. CT26 cells were subcutaneously inoculated in the right armpit of each group with 5 脳 105 CT26 cells. Six experimental groups (group A) were intraperitoneally injected with 100.00m 50.0010. 00 (1.00) and 0.01 渭 g / d EsSPN once a day 脳 5 days, and the control group were injected with the same amount of PBSs intraperitoneally. The mice in the control group were intraperitoneally injected with the same amount of PBSs. The tumor diameter was measured on the 7th day after inoculation. The relative tumor volume and the relative tumor proliferation rate were calculated. On the 25th day after inoculation, 5 mice were killed in each group, the tumor weight was weighed and the tumor inhibition rate was calculated. The effect of EsSP on lymphocyte proliferation in tumor-bearing mice was determined by MTS colorimetric assay and flow cytometry was used to detect the ratio of CD _ 4 ~ / CD _ 8 ~ T lymphocytes in peripheral blood. The time and number of death of tumor-bearing mice were recorded and observed for 80 days. Single factor analysis of variance (ANOVA) was used to compare the differences among groups. Results the number of tumor cells was 5 脳 10 ~ 5. 鎺ョ緇撹偁鐧岀粏鑳?yōu)鍚幗W，

本文編號：2029679