本文選題：肝細(xì)胞肝癌 + Beclin-1�。� 參考：《青島大學(xué)》2017年碩士論文

【摘要】：目的探討自噬相關(guān)蛋白Beclin-1和凋亡蛋白Caspase-9以及p53在肝細(xì)胞肝癌(HCC)組織中的表達(dá)及其意義,結(jié)合臨床病理資料分析Beclin-1、Caspase-9及p53在HCC發(fā)生發(fā)展過程中的作用及三者之間的相關(guān)關(guān)系,并對患者進(jìn)行預(yù)后分析,為HCC的早期檢測、治療及預(yù)后提供更為有效的依據(jù)。方法收集HBV病毒相關(guān)HCC石蠟標(biāo)本108例、肝硬化石蠟標(biāo)本30例及正常肝組織石蠟標(biāo)本20例,采用免疫組織化學(xué)染色方法分別檢測三組標(biāo)本中Beclin-1及Caspase-9、p53的表達(dá)情況并分析它們與HCC臨床病理參數(shù)及患者五年生存時間及無瘤生存時間之間的關(guān)系。結(jié)果Beclin-1在HCC組中的陽性表達(dá)率明顯低于正常肝組織組,差異有統(tǒng)計學(xué)意義(χ2=5.575,P=0.016),肝硬化組和正常肝組織組,肝硬化組和HCC組的陽性表達(dá)率均無統(tǒng)計學(xué)差異(χ2=3.704,P=0.054;χ2=0.054,P=0.815);p53在HCC組中的陽性表達(dá)率高于正常肝組織組和肝硬化組,差異有統(tǒng)計學(xué)意義(χ2=17.297,P=0.000;χ2=21.330,P=0.000);Caspase-9在HCC中的表達(dá)明顯高于正常肝臟組織(χ2=4.349,P=0.037),而在肝硬化組織與正常肝臟組織及肝硬化組織與HCC組織之間,其表達(dá)無顯著差異(χ2=0.347,P=0.556;χ2=2.713,P=0.100)。Beclin-1在HCC中的表達(dá)與Edmondson病理分級(P=0.010)及AFP的高低(P=0.047)有關(guān),與患者的年齡、性別、腫瘤的大小、數(shù)目以及是否有衛(wèi)星灶、血管癌栓、肝硬化、被膜侵犯、TNM分期均無關(guān)(P0.05);p53在HCC中的表達(dá)與Edmondson病理分級有關(guān)(P=0.001),與患者的年齡、性別、腫瘤的大小、數(shù)目、AFP的高低以及是否有衛(wèi)星灶、血管癌栓、肝硬化、被膜侵犯、TNM分期均無關(guān)(P0.05);Caspase-9在HCC中的表達(dá)與Edmondson病理分級(P=0.001)及腫瘤是否有衛(wèi)星灶(P=0.005)密切相關(guān),與患者的年齡、性別、AFP的高低以及腫瘤的大小、數(shù)目、是否有血管癌栓、肝硬化、被膜侵犯、TNM分期無關(guān)。HCC組織中Beclin-1與p53的表達(dá)呈負(fù)相關(guān)(r=-0241,P=0.012),Beclin-1與Caspase-9的表達(dá)呈正相關(guān)(r=0.223,P=0.020),Caspase-9與p53在HCC中的表達(dá)不相關(guān),沒有統(tǒng)計學(xué)差異(χ2=5.775,P=0.077)。預(yù)后分析Beclin-1、p53及Caspase-9對患者預(yù)后的影響,發(fā)現(xiàn)其與患者預(yù)后有關(guān)。Beclin-1高表達(dá)的病例其生存時間及無瘤生存時間均長于低表達(dá)的病例(P=0.001,P=0.006),Caspase-9陽性表達(dá)的病例其生存時間及無瘤生存時間均長于陰性表達(dá)的病例(P=0.025);p53陰性表達(dá)的病例,生存時間要長于陽性表達(dá)的病例(P=0.025),但對于無瘤生存時間沒有顯著差異(P=0.084)。結(jié)論在肝細(xì)胞肝癌組織中Beclin-1低表達(dá),Caspase-9與p53均高表達(dá),Beclin-1與p53的表達(dá)呈負(fù)相關(guān),與Caspase-9的表達(dá)呈正相關(guān)。Beclin-1及p53可能與HCC的發(fā)生有關(guān),Caspase-9可能與HCC的發(fā)生及發(fā)展均有關(guān)。Beclin-1、p53及Caspase-9可作為判斷肝細(xì)胞肝癌生物學(xué)行為及預(yù)后的重要指標(biāo)。
[Abstract]:Objective to investigate the expression and significance of the autophagy associated protein Beclin-1, apoptosis protein Caspase-9 and p53 in hepatocellular carcinoma (HCC), and to analyze the role of Beclin-1Caspase-9 and p53 in the development of HCC and their correlation with the clinicopathological data. The prognosis of HCC was analyzed to provide a more effective basis for early detection, treatment and prognosis of HCC. Methods 108 cases of HBV virus-associated HCC, 30 cases of liver cirrhosis and 20 cases of normal liver tissue were collected. The expressions of Beclin-1 and Caspase-9 p53 were detected by immunohistochemical staining in three groups, and their relationship with clinicopathological parameters, 5-year survival time and tumor-free survival time of HCC were analyzed. Results the positive expression rate of Beclin-1 in HCC group was significantly lower than that in normal liver tissue group (蠂 ~ 2 / 5.575P ~ (0.016), liver cirrhosis group and normal liver tissue group. There was no significant difference in the positive expression of p53 between the liver cirrhosis group and the HCC group (蠂 ~ 2 / 3.704 / P ~ (0.054), 蠂 ~ (2 +) ~ (0.054) P ~ (0.815) p53 expression in the HCC group, which was higher than that in the normal liver tissue group and the liver cirrhosis group. The expression of Caspase-9 in HCC was significantly higher than that in normal liver tissue (蠂 ~ 2 ~ 2 ~ (4.349) P ~ (0.037), but between liver cirrhosis tissue and normal liver tissue and liver cirrhosis tissue and HCC tissue, the expression of Caspase-9 in HCC was significantly higher than that in normal liver tissue (蠂 ~ (2) (蠂 ~ (2) (蠂 ~ (2) 17.297 (P < 0.05), and the expression of Caspase-9 in HCC was higher than that in normal liver tissue (蠂 ~ (2). There was no significant difference in its expression (蠂 ~ 2 / 0.347P ~ (0.556); 蠂 ~ (2 +) 2.713P ~ (0.100). Beclin-1 expression in HCC was related to Edmondson's pathological grade (P ~ (0.010) and AFP (P ~ (0.047). It was associated with age, sex, tumor size, number and satellite foci, vascular tumor thrombus, cirrhosis, etc. The expression of p53 in HCC was not related to the pathological grade of Edmondson. It was associated with age, sex, tumor size, number of AFP and whether there were satellite foci, vascular thrombus, cirrhosis. The expression of Caspase-9 in HCC was not correlated with the pathological grade of Edmondson (P0. 001) and whether there was a satellite tumor (P0. 005). It was associated with age, sex of AFP, size and number of tumor, whether there were vascular thrombus and cirrhosis. There was no correlation between the expression of Beclin-1 and p53 in HCC. There was a positive correlation between the expression of Beclin-1 and Caspase-9 in HCC. The effect of Beclin-1 p53 and Caspase-9 on the prognosis was analyzed. It was found that the survival time and tumor-free survival time of the patients with high expression of .Beclin-1 related to the prognosis of patients were longer than those of the cases with low expression of P0. 001 and P0. 006. The survival time and tumor-free survival time of the cases with positive expression of Caspase-9 were longer than those with negative expression of P0. 025 and p53. The survival time was longer than that in the positive cases, but there was no significant difference in the survival time without tumor. Conclusion the low expression of Beclin-1 in hepatocellular carcinoma and the high expression of Caspase-9 and p53 are negatively correlated with the expression of p53. The expression of Caspase-9 was positively correlated with the expression of Caspase-9. Beclin-1 and p53 may be related to the occurrence and development of HCC. Beclin-1 p53 and Caspase-9 may be important indexes to judge the biological behavior and prognosis of HCC.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R735.7

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊靜;王明娟;程玉;顏勇;齊潔敏;;胃癌組織中Caspase 3、Caspase 9的表達(dá)及其臨床意義[J];臨床與實驗病理學(xué)雜志;2016年10期

2 艾航宇;邱學(xué)德;;前列腺癌病理特征與腫瘤組織中MACC1、c-Met、Apaf-1和Caspase-9表達(dá)的關(guān)系[J];海南醫(yī)學(xué)院學(xué)報;2016年16期

3 許斌;孟媛;姜彥多;;自噬相關(guān)基因Beclin-1與抑癌基因PTEN、p53在大腸癌中的表達(dá)和意義[J];現(xiàn)代腫瘤醫(yī)學(xué);2015年04期

4 木爾扯爾;文彬;黃一凡;劉鈞;;結(jié)腸癌中caspase9表達(dá)程度與癌周炎癥程度關(guān)系的研究[J];川北醫(yī)學(xué)院學(xué)報;2014年03期

5 陳國昆;吳益華;張世周;張東升;黃圣運;溫潔;;p53和自噬相關(guān)基因Beclinl在唾液腺多形性腺瘤和癌在多形性腺瘤中的表達(dá)及意義[J];上�？谇会t(yī)學(xué);2014年02期

6 郭曉東;李志偉;楊美;熊璐;;Caspase-9和Survivin在肝癌組織中的表達(dá)及意義[J];臨床肝膽病雜志;2014年03期

7 何雙;吳淑華;胡金龍;溫菲菲;孫晨博;;自噬調(diào)控因子beclin1及mTOR在大腸腺瘤癌變過程中的表達(dá)及意義[J];世界華人消化雜志;2014年07期

8 汪亞航;俞耀軍;;甲狀腺癌與腺瘤組織Caspase-9表達(dá)及其臨床意義的探討[J];中華腫瘤防治雜志;2013年05期

9 郭曉東;皋月娟;萬維;李瑞生;周艷賢;趙景民;段學(xué)章;李進(jìn);;原發(fā)性肝細(xì)胞癌組織中Beclin 1的表達(dá)及其意義[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2013年01期

10 鄭海燕;王興芬;孫保存;張曉陽;許麗萍;;大腸癌發(fā)生發(fā)展中自噬基因Beclin1的表達(dá)及其與增殖和凋亡相關(guān)基因的關(guān)系探討[J];中國腫瘤臨床;2011年11期

，

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