本文選題：原位種植性肝癌 + 血管內皮生長因子��； 參考：《中山大學學報(醫(yī)學科學版)》2017年03期

【摘要】：【目的】探索sorafenib(索拉非尼)在不同干預時機中對血管內皮生長因子(VEGF)促瘤效應的抑制作用�！痉椒ā繉�50只SD大鼠肝臟接種Wakler256腫瘤細胞形成的裸鼠皮下瘤塊,10 d后行MR掃描,選取建模成功的40只SD大鼠隨機分成4組,每組10只:A組,對照組,僅注射VEGF;B組,sorafenib先于VEGF 72 h導入組;C組,sorafenib與VEGF同時導入組;D組,sorafenib后于VEGF 72 h導入組。觀察并比較4組實驗動物腫瘤的生長及中位生存時間。待實驗動物死亡后,采用免疫組織化學法檢測各組大鼠肝腫瘤的VEGF的表達�！窘Y果】服用sorafenib 10 d后,MR顯示各組大鼠肝臟腫瘤明顯增大,sorafenib組腫瘤大小小于對照組(5.40 cm),具有統(tǒng)計學意義(P0.05)。四組實驗動物中位生存時間分別是(19.6±1.8)d、(31.2±7.0)d、(27.4±4.9)d、(26.5±4.6)d。服用sorafenib組大鼠生存時間明顯長于對照組(P0.05)。將死亡實驗大鼠進行HE染色及免疫組織化學檢查,四組實驗動物的VEGF值分別為88.3±13.6、42.8±8.0、71.9±15.7、73.6±13.7,sorafenib組與對照組數(shù)值差異具有明顯統(tǒng)計學意義(P0.05),且不同sorafenib組之間數(shù)值差異較大�！窘Y論】Sorafenib能延長肝腫瘤大鼠的生存時間,減少腫瘤血管生成。Sorafenib服用時間早于VEGF一過性升高前的生存獲益明顯好于VEGF升高后服用。
[Abstract]:[objective] to investigate the inhibitory effect of sorafenibil on vascular endothelial growth factor (VEGF) in different intervention time. [methods] 50 SD rats were inoculated with subcutaneous tumor cells of Wakler256 tumor cells in nude mice for 10 days. Forty SD rats were randomly divided into 4 groups, 10 rats in each group and 10 rats in control group. Only group VEGFN B was injected with sorafenib before VEGF 72 h in group C and group C was introduced with VEGF at the same time as group D and then group D was injected with sorafenib at 72 h before VEGF was introduced at 72 h. The growth and median survival time of tumor in 4 groups were observed and compared. After the death of the experimental animal, Immunohistochemical method was used to detect the expression of sorafenib in the liver tumors of rats in each group. [results] after 10 days of administration of sorafenib, Mr showed that the tumor size of the group was significantly larger than that of the control group (5.40 cm ~ (-1), which had statistical significance (P 0.05). The median survival time of the experimental animals in the four groups was 19.6 鹵1.8g / d, 31.2 鹵7.0g / d, 27.4 鹵4.9g / d, 26.5 鹵4.6 / d, respectively. The survival time of sorafenib group was longer than that of control group (P 0.05). The death rats were stained with HE and immunohistochemistry. The VEGFvalues of the experimental animals in the four groups were 88.3 鹵13.6 鹵42.8 鹵8.0 鹵71.9 鹵15.77.73.6 鹵13.7 respectively, and there were significant differences between the sorafenib groups and the control group. [conclusion] Sorafenib can prolong the survival time of the rats with liver tumor, and the difference is significant among the four groups. [conclusion] Sorafenib can prolong the survival time of the rats with liver tumor. The survival benefit of decreasing tumor angiogenesis. Sorafenib was significantly better than that of VEGF.
【作者單位】： 中山大學附屬第一醫(yī)院放射介入科;
【基金】：吳階平醫(yī)學基金(320.6750.14800)
【分類號】：R735.7

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