本文選題：結(jié)直腸癌 + Arpin��； 參考：《青島大學(xué)》2017年碩士論文

【摘要】：目的:檢測Arpin在結(jié)直腸癌及正常結(jié)直腸組織中的表達(dá)情況,并探討其與結(jié)直腸癌臨床病理特征的關(guān)系及其異常表達(dá)的臨床意義。方法:收集2015年3月—2015年12月于青島市市立醫(yī)院結(jié)直腸外科行結(jié)直腸癌根治術(shù),并經(jīng)術(shù)后病理診斷證實(shí)為結(jié)直腸癌的所有患者,根據(jù)入組標(biāo)準(zhǔn),最終選取80例作為研究對象。根據(jù)患者臨床病理特征的不同,將80例結(jié)直腸癌患者分為不同組。用于實(shí)驗(yàn)的結(jié)直腸組織取自手術(shù)切除標(biāo)本。另外22例石蠟包埋的正常結(jié)直腸組織塊(距離癌組織邊緣超過5cm)作為對照組。采用免疫組織化學(xué)法(IHC)檢測Arpin蛋白在結(jié)直腸癌組織及正常結(jié)直腸組織中的表達(dá),同時運(yùn)用實(shí)時熒光定量PCR(q RT-PCR)分別對80對結(jié)直腸癌組織及與其配對的癌旁組織中的Arpin m RNA進(jìn)行檢測。結(jié)果:Arpin蛋白在結(jié)直腸癌組織中的表達(dá)水平明顯低于正常結(jié)直腸組織(32.50%vs86.36%,?2=20.306,P0.001)。對80例經(jīng)過免疫組化分析的結(jié)直腸癌樣本進(jìn)行卡方分析表明,在有無淋巴結(jié)轉(zhuǎn)移(81.82%vs 50.00%,?2=9.138,P=0.003)及遠(yuǎn)處轉(zhuǎn)移(89.47%vs 60.66%,?2=5.484,P=0.019)的腫瘤組織中Arpin蛋白的表達(dá)水平不同,差別具有統(tǒng)計(jì)學(xué)意義。Arpin蛋白在不同TNM分期的結(jié)直腸癌患者的癌組織中表達(dá)水平也不同,差別具有統(tǒng)計(jì)學(xué)意義(?2=26.901,P0.001)。實(shí)時熒光定量PCR結(jié)果顯示,結(jié)直腸癌組織中Arpin m RNA的表達(dá)水平明顯低于與其配對的癌旁組織(P0.001)。結(jié)論:結(jié)直腸癌組織中Arpin的低表達(dá)水平與有無淋巴結(jié)轉(zhuǎn)移、遠(yuǎn)處轉(zhuǎn)移及TNM分期有關(guān),Arpin表達(dá)水平的降低可能導(dǎo)致腫瘤侵襲和轉(zhuǎn)移能力的獲取。
[Abstract]:Aim: to detect the expression of Arpin in colorectal cancer and normal colorectal tissues, and to explore the relationship between Arpin and clinicopathological features of colorectal cancer and its clinical significance. Methods: from March 2015 to December 2015, all the patients with colorectal cancer proved to be colorectal cancer by pathological diagnosis were collected from Qingdao City Hospital. According to the criteria of admission, 80 patients were selected as the study objects. According to the clinicopathological characteristics of the patients, 80 patients with colorectal cancer were divided into different groups. The colorectal tissue used in the experiment was obtained from surgical specimens. Another 22 cases of paraffin embedded normal colorectal tissue mass (more than 5 cm from the edge of cancer tissue) served as control group. Immunohistochemical method was used to detect the expression of Arpin protein in colorectal cancer tissues and normal colorectal tissues. At the same time, the Arpin mRNA in 80 pairs of colorectal cancer tissues and their matched adjacent tissues were detected by real-time fluorescence quantitative PCRQ RT-PCR. Results the expression of the protein in colorectal carcinoma was significantly lower than that in normal colorectal tissue (n = 32.50). The chi-square analysis of 80 colorectal cancer samples with immunohistochemical analysis showed that the expression of Arpin protein was different in the tumor tissues with or without lymph node metastasis (81.82 vs 50.0029.138P0. 003) and distant metastasis (89.47 vs 60.66484 P0. 019). The expression level of Arpin protein in colorectal cancer tissues with different TNM stages was also different, and the difference was statistically significant (P 0.001). The results of real-time fluorescence quantitative PCR showed that the expression level of Arpin mRNA in colorectal cancer tissues was significantly lower than that in adjacent tissues (P0.001). Conclusion: the low expression level of Arpin is associated with lymph node metastasis, distant metastasis and decreased expression of Arpin in colorectal carcinoma, which may lead to the acquisition of tumor invasion and metastasis.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R735.3

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