本文選題：皮層肌動(dòng)蛋白 + 肺癌��； 參考：《華中科技大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年04期

【摘要】：目的探討皮層肌動(dòng)蛋白(Cortactin)在肺癌組織的表達(dá)與患者生存預(yù)后的關(guān)系及對(duì)肺癌細(xì)胞侵襲、遷移的影響。方法收集貴州省人民醫(yī)院50例行肺葉切除術(shù)的非小細(xì)胞肺癌(NSCLC)患者癌組織及癌旁組織,免疫組化染色檢測(cè)肺癌組織Cortactin表達(dá),分析Cortactin表達(dá)與肺癌患者臨床資料的關(guān)系。對(duì)肺癌患者進(jìn)行隨訪,記錄患者5年生存率;以5年生存率作為評(píng)價(jià)指標(biāo),采用單變量和多變量Cox比例風(fēng)險(xiǎn)模型評(píng)價(jià)患者預(yù)后的影響因素。采用小干擾RNA(siRNA)技術(shù)抑制肺癌A549細(xì)胞Cortactin的表達(dá),劃痕實(shí)驗(yàn)檢測(cè)細(xì)胞遷移能力,Transwell實(shí)驗(yàn)檢測(cè)細(xì)胞侵襲能力。結(jié)果肺癌組織Cortactin高表達(dá)率為72.0%(36/50),癌旁組織高表達(dá)率為24.0%(12/50),肺癌組織Cortactin高表達(dá)率明顯高于癌旁組織,兩組間比較差異具有統(tǒng)計(jì)學(xué)意義(P0.01)。腫瘤最大徑≥3cm組Cortactin高表達(dá)率明顯高于腫瘤最大徑3cm組,中高分化組Cortactin高表達(dá)率明顯高于低分化組,有淋巴結(jié)轉(zhuǎn)移組Cortactin高表達(dá)率明顯高于無(wú)淋巴結(jié)轉(zhuǎn)移組(均P0.05);Cortactin表達(dá)與性別、年齡、TNM分期、組織學(xué)類型無(wú)關(guān)(均P0.05)。Cortactin低表達(dá)組5年總生存率為42.1%,高表達(dá)組5年總生存率為13.7%,高表達(dá)組5年總生存率明顯低于低表達(dá)組(P=0.018)。Cox多因素分析顯示腫瘤直徑大、淋巴結(jié)轉(zhuǎn)移、高Cortactin表達(dá)是影響患者總生存期的獨(dú)立危險(xiǎn)因素(均P0.05)。劃痕實(shí)驗(yàn)顯示Cortactin-siRNA組細(xì)胞遷移率明顯小于Cortactin-NC組、Cortactin-BC組;Transwell實(shí)驗(yàn)顯示CortactinsiRNA組穿膜細(xì)胞數(shù)明顯少于Cortactin-NC組、Cortactin-BC組,差異均具有統(tǒng)計(jì)學(xué)意義(均P0.05)。結(jié)論 Cortactin表達(dá)與肺癌患者生存預(yù)后有關(guān),抑制Cortactin表達(dá)能夠降低肺癌細(xì)胞的侵襲、遷移能力,Cortactin有望成為肺癌治療的新靶點(diǎn)。
[Abstract]:Objective to investigate the relationship between the expression of cortical actin Cortacin and the survival and prognosis of patients with lung cancer and its effect on the invasion and migration of lung cancer cells. Methods the tissues and adjacent tissues of 50 patients with non-small cell lung cancer (NSCLC) undergoing lobectomy in Guizhou Provincial people's Hospital were collected. The expression of Cortactin in lung cancer tissues was detected by immunohistochemical staining. The relationship between the expression of Cortactin and the clinical data of patients with lung cancer was analyzed. The 5-year survival rate was recorded and univariate and multivariate Cox proportional risk models were used to evaluate the prognostic factors of lung cancer patients. Small interference RNA-siRNAs were used to inhibit the expression of Cortactin in lung cancer A549 cells. The migration ability of A549 cells was detected by scratch test and the invasion ability was detected by Transwell assay. Results the high expression rate of Cortactin in lung cancer tissues was 72.0 / 36 / 50 and that in adjacent tissues was 24.0 / 12 / 50. The high expression rate of Cortactin in lung cancer tissues was significantly higher than that in paracancerous tissues. The difference between the two groups was statistically significant (P 0.01). The high expression rate of Cortactin was significantly higher in the group of tumor maximum diameter 鈮，

本文編號(hào)：1990431