本文選題：SB203580 + 薯蕷皂素　； 參考：《江蘇大學》2016年碩士論文

【摘要】：目的:進一步探討薯蕷皂素(Diosgenin,Dio)對人食管癌Eca109細胞的影響的作用機制。方法:體外培養(yǎng)人食管鱗狀上皮細胞癌Eca109細胞,用p38特異性抑制劑SB203580(10μg/m L)處理Eca109細胞,Western blot方法用于檢測SB203580處理后p38 MAPK蛋白表達水平。SB203580(10μg/m L)和薯蕷皂素(50μg/m L)共處理Eca109細胞,MTT實驗用于檢測兩藥共處理后Eca109細胞的增殖情況,Western blot方法檢測兩藥共處理后的Eca109細胞的凋亡情況及磷酸化p38(p-p38MAPK)的蛋白表達水平。結果:在人食管癌Eca109細胞中,p38特異性抑制劑SB203580能有效抑制p38蛋白的表達,Eca109細胞經(jīng)過50μg/m L的薯蕷皂素和10μg/m L的SB203580處理后,與未使用抑制劑處理組相比,Eca109細胞的增殖進一步被抑制,細胞的凋亡效應進一步增強,p-p38蛋白的表達水平進一步降低。結論:薯蕷皂素調控人食管癌Eca109細胞的增殖,凋亡過程可能是通過p-p38MAPK途徑發(fā)揮作用的。
[Abstract]:Aim: to investigate the mechanism of diosgenin (Dio) on human esophageal carcinoma Eca109 cells. Methods: human esophageal squamous cell carcinoma (Eca109) cells were cultured in vitro. Treatment of Eca109 cells with p38 specific inhibitor SB203580(10 渭 g / mL Western blot method was used to detect the expression level of p38 MAPK protein. SB20358010 渭 g / mL) and diosgenin 50 渭 g / mL co-treated Eca109 cells were used to detect the proliferation of Eca109 cells after co-treatment with two drugs. The apoptosis of Eca109 cells and the protein expression of phosphorylated p38 MAPK were detected by Western blot. Results: SB203580, a specific inhibitor of p38, could effectively inhibit the expression of p38 protein in human esophageal carcinoma Eca109 cells. After treated with 50 渭 g / mL diosgenin and 10 渭 g / mL SB203580, the proliferation of Eca109 cells was further inhibited. The apoptotic effect further enhanced the expression of p-p38 protein and further decreased the expression level of p-p38 protein. Conclusion: diosgenin regulates the proliferation and apoptosis of human esophageal carcinoma Eca109 cells through p-p38MAPK pathway.
【學位授予單位】：江蘇大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R735.1

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