天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

當前位置:主頁 > 醫(yī)學論文 > 腫瘤論文 >

MIR155HG促進人肺腺癌細胞A549的增殖和遷移侵襲

發(fā)布時間:2018-06-06 14:55

  本文選題:長鏈非編碼RNA + MIRHG ; 參考:《重慶醫(yī)科大學學報》2017年01期


【摘要】:目的:探討非編碼RNA MIR155HG對人肺癌A549細胞增殖、遷移和侵襲能力的影響及機制。方法:過表達或敲減MIR155HG后,MTS檢測A549細胞生長的變化,流氏細胞儀檢測細胞周期。Transwell遷移和侵襲實驗檢測過表達或敲減MIR155HG后,A549細胞遷移和侵襲能力的變化。過表達MIR155HG后,定量PCR檢測A549細胞中mi R-155-5p的表達。結(jié)果:MTS法顯示,轉(zhuǎn)染72 h和96 h后,過表達MIR155HG組細胞吸光度(absorbance,A)值分別為(2.47±0.14)和(3.13±0.15),均較對照組[(2.09±0.12)(72 h)和(2.50±0.13)(96 h)]增加(P=0.006,P=0.027);敲減MIR155HG組細胞在48、72和96 h的OD值分別為(1.69±0.15),(1.87±0.09)和(2.24±0.16),較對照組[(2.04±0.06)(48 h),(2.43±016)(72 h)和(2.88±0.11)(96 h)]均降低(P=0.006,P=0.006和P=0.004);敲減MIR155HG組的A549細胞G0/G1期比例為63.93%,與對照組(54.32%)相比,增加了9.61%;遷移實驗結(jié)果顯示,過表達MIR155HG組的穿膜細胞數(shù)為(31.67±3.51)個,與對照組(12.67±2.51)相比增加明顯(P=0.002)。敲減MIR155HG組的A549細胞的穿膜細胞數(shù)為(13.67±3.21)個,與對照組(36.00±4.58)相比明顯減少(P=0.002)。侵襲實驗結(jié)果顯示,過表達MIR155HG組細胞穿膜細胞數(shù)為(42.33±7.02)個,與對照組(15.67±3.51)相比明顯增加(P=0.004)。敲減MIR155HG組穿膜細胞數(shù)為(15.00±3.60)個,與對照組(39.00±4.36)相比明顯減少(P=0.002)。定量PCR顯示,過表達MIR155HG后,mi R-155-5p的表達較對照組增加的倍數(shù)為(17.99±2.42)(P=0.000)。結(jié)論:MIR155HG能增強A549細胞的增殖、遷移和侵襲能力,其發(fā)揮作用的機制可能是通過產(chǎn)生mi R-155-5p來實現(xiàn)。
[Abstract]:Objective: To investigate the effect and mechanism of non coded RNA MIR155HG on proliferation, migration and invasion of human lung cancer A549 cells. Methods: after overexpression or knockout MIR155HG, MTS detected the changes in growth of A549 cells. The flow cytometer detected the expression of.Transwell migration and invasion in the cell cycle, and after the knockout of MIR155HG, and the migration and invasion of A549 cells. After overexpression of MIR155HG, quantitative PCR was used to detect the expression of MI R-155-5p in A549 cells. Results: MTS method showed that after transfection of 72 h and 96 h, the values of overexpressed MIR155HG group cell absorbency (absorbance, A) were (2.47 + 0.14) and (3.13 + 0.15) respectively, which were higher than those of the control group (2.09 + 0.12) (72 h) and (2.50 + 0.13) (96)]. The OD values of MIR155HG group at 48,72 and 96 h were (1.69 + 0.15), (1.87 + 0.09) and (2.24 + 0.16), respectively, compared with the control group [(2.04 + 0.06) (48 h), (2.43 + 016) (72 h) and (P=0.006) (P=0.006 and P=0.004)), and the A549 cell G0/G1 phase ratio of the knockout MIR155HG group was higher than that of the control group. The migration experiment showed that the number of transmembrane cells in the overexpressed MIR155HG group was (31.67 + 3.51), and was significantly increased compared with the control group (12.67 + 2.51). The number of membrane cells in the A549 cells of the MIR155HG group was (13.67 + 3.21), compared with the control group (36 + 4.58). The results of the invasion experiment showed that the overexpression of MIR155H was MIR155H. The number of cell transmembrane cells in the G group was (42.33 + 7.02), compared with the control group (15.67 + 3.51) (P=0.004). The number of membrane cells in the subtraction MIR155HG group was (15 + 3.60), compared with the control group (39 + 4.36). The quantitative PCR showed that the expression of MI R-155-5p was increased by (17.99 + 2) than that of the control group after MIR155HG. .42) (P=0.000). Conclusion: MIR155HG can enhance the proliferation, migration and invasion of A549 cells, and its mechanism may be achieved through the production of MI R-155-5p.
,

本文編號:1987040

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/yixuelunwen/zlx/1987040.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶f13a9***提供,本站僅收錄摘要或目錄,作者需要刪除請E-mail郵箱bigeng88@qq.com
国产户外勾引精品露出一区| 永久福利盒子日韩日韩| 污污黄黄的成年亚洲毛片| 久久99午夜福利视频| 国产又大又硬又粗又黄| 东北老熟妇全程露脸被内射| 日韩中文字幕免费在线视频| 69精品一区二区蜜桃视频| 久久99夜色精品噜噜亚洲av | 欧美一区二区三区99| 黄色激情视频中文字幕| 国产一级二级三级观看| 日韩一级欧美一级久久| 日韩一区二区三区高清在| 黑丝袜美女老师的小逼逼| 日韩人妻中文字幕精品| 午夜福利网午夜福利网| 日韩精品你懂的在线观看| 91久久精品在这里色伊人| 久久福利视频在线观看 | 空之色水之色在线播放| 日韩国产精品激情一区| 老熟女露脸一二三四区| 国产精品九九九一区二区| 激情三级在线观看视频| 亚洲女同一区二区另类| 欧美日韩国产精品自在自线| 欧美黑人巨大一区二区三区| 国产亚洲视频香蕉一区| 日本不卡一本二本三区| 后入美臀少妇一区二区| 日本精品啪啪一区二区三区| 国产精品美女午夜福利| 大香蕉伊人一区二区三区| 在线免费国产一区二区| 欧洲日韩精品一区二区三区| 国内真实露脸偷拍视频| 国产欧美日韩精品自拍| 中文字幕中文字幕一区二区| 五月天丁香婷婷狠狠爱| 亚洲人妻av中文字幕|