本文選題：長(zhǎng)鏈非編碼RNA + MIRHG�。� 參考：《重慶醫(yī)科大學(xué)學(xué)報(bào)》2017年01期

【摘要】：目的:探討非編碼RNA MIR155HG對(duì)人肺癌A549細(xì)胞增殖、遷移和侵襲能力的影響及機(jī)制。方法:過表達(dá)或敲減MIR155HG后,MTS檢測(cè)A549細(xì)胞生長(zhǎng)的變化,流氏細(xì)胞儀檢測(cè)細(xì)胞周期。Transwell遷移和侵襲實(shí)驗(yàn)檢測(cè)過表達(dá)或敲減MIR155HG后,A549細(xì)胞遷移和侵襲能力的變化。過表達(dá)MIR155HG后,定量PCR檢測(cè)A549細(xì)胞中mi R-155-5p的表達(dá)。結(jié)果:MTS法顯示,轉(zhuǎn)染72 h和96 h后,過表達(dá)MIR155HG組細(xì)胞吸光度(absorbance,A)值分別為(2.47±0.14)和(3.13±0.15),均較對(duì)照組[(2.09±0.12)(72 h)和(2.50±0.13)(96 h)]增加(P=0.006,P=0.027);敲減MIR155HG組細(xì)胞在48、72和96 h的OD值分別為(1.69±0.15),(1.87±0.09)和(2.24±0.16),較對(duì)照組[(2.04±0.06)(48 h),(2.43±016)(72 h)和(2.88±0.11)(96 h)]均降低(P=0.006,P=0.006和P=0.004);敲減MIR155HG組的A549細(xì)胞G0/G1期比例為63.93%,與對(duì)照組(54.32%)相比,增加了9.61%;遷移實(shí)驗(yàn)結(jié)果顯示,過表達(dá)MIR155HG組的穿膜細(xì)胞數(shù)為(31.67±3.51)個(gè),與對(duì)照組(12.67±2.51)相比增加明顯(P=0.002)。敲減MIR155HG組的A549細(xì)胞的穿膜細(xì)胞數(shù)為(13.67±3.21)個(gè),與對(duì)照組(36.00±4.58)相比明顯減少(P=0.002)。侵襲實(shí)驗(yàn)結(jié)果顯示,過表達(dá)MIR155HG組細(xì)胞穿膜細(xì)胞數(shù)為(42.33±7.02)個(gè),與對(duì)照組(15.67±3.51)相比明顯增加(P=0.004)。敲減MIR155HG組穿膜細(xì)胞數(shù)為(15.00±3.60)個(gè),與對(duì)照組(39.00±4.36)相比明顯減少(P=0.002)。定量PCR顯示,過表達(dá)MIR155HG后,mi R-155-5p的表達(dá)較對(duì)照組增加的倍數(shù)為(17.99±2.42)(P=0.000)。結(jié)論:MIR155HG能增強(qiáng)A549細(xì)胞的增殖、遷移和侵襲能力,其發(fā)揮作用的機(jī)制可能是通過產(chǎn)生mi R-155-5p來實(shí)現(xiàn)。
[Abstract]:Objective: To investigate the effect and mechanism of non coded RNA MIR155HG on proliferation, migration and invasion of human lung cancer A549 cells. Methods: after overexpression or knockout MIR155HG, MTS detected the changes in growth of A549 cells. The flow cytometer detected the expression of.Transwell migration and invasion in the cell cycle, and after the knockout of MIR155HG, and the migration and invasion of A549 cells. After overexpression of MIR155HG, quantitative PCR was used to detect the expression of MI R-155-5p in A549 cells. Results: MTS method showed that after transfection of 72 h and 96 h, the values of overexpressed MIR155HG group cell absorbency (absorbance, A) were (2.47 + 0.14) and (3.13 + 0.15) respectively, which were higher than those of the control group (2.09 + 0.12) (72 h) and (2.50 + 0.13) (96)]. The OD values of MIR155HG group at 48,72 and 96 h were (1.69 + 0.15), (1.87 + 0.09) and (2.24 + 0.16), respectively, compared with the control group [(2.04 + 0.06) (48 h), (2.43 + 016) (72 h) and (P=0.006) (P=0.006 and P=0.004)), and the A549 cell G0/G1 phase ratio of the knockout MIR155HG group was higher than that of the control group. The migration experiment showed that the number of transmembrane cells in the overexpressed MIR155HG group was (31.67 + 3.51), and was significantly increased compared with the control group (12.67 + 2.51). The number of membrane cells in the A549 cells of the MIR155HG group was (13.67 + 3.21), compared with the control group (36 + 4.58). The results of the invasion experiment showed that the overexpression of MIR155H was MIR155H. The number of cell transmembrane cells in the G group was (42.33 + 7.02), compared with the control group (15.67 + 3.51) (P=0.004). The number of membrane cells in the subtraction MIR155HG group was (15 + 3.60), compared with the control group (39 + 4.36). The quantitative PCR showed that the expression of MI R-155-5p was increased by (17.99 + 2) than that of the control group after MIR155HG. .42) (P=0.000). Conclusion: MIR155HG can enhance the proliferation, migration and invasion of A549 cells, and its mechanism may be achieved through the production of MI R-155-5p.
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本文編號(hào)：1987040