本文選題：姜黃素 + 順鉑��； 參考：《中國醫(yī)科大學(xué)學(xué)報(bào)》2017年06期

【摘要】：目的姜黃素或姜黃素聯(lián)合順鉑在體外作用于人舌鱗癌Tca-8113細(xì)胞,觀察其對人舌鱗癌細(xì)胞增殖的影響,并探討可能的抗腫瘤作用機(jī)制。方法將培養(yǎng)的Tca-8113細(xì)胞隨機(jī)分為姜黃素組、順鉑組、姜黃素聯(lián)合順鉑組和對照組。MTT法檢測各組藥物對舌鱗癌Tca-8113細(xì)胞增殖的抑制作用;Hoechst 33258染色法觀察各組藥物對舌鱗癌細(xì)胞核的形態(tài)影響;流式細(xì)胞術(shù)檢測細(xì)胞凋亡率;Western blotting檢測各組藥物對舌鱗癌Tca-8113細(xì)胞Notch1和表皮生長因子受體(EGFR)蛋白表達(dá)的影響。結(jié)果 MTT法結(jié)果顯示,姜黃素聯(lián)合順鉑組抑制舌鱗癌Tca-8113細(xì)胞增殖的作用明顯高于姜黃素組和順鉑組(P0.05)。流式細(xì)胞分析結(jié)果顯示,姜黃素聯(lián)合順鉑組的細(xì)胞凋亡率明顯高于姜黃素組和順鉑組(P0.05)。Western blotting結(jié)果顯示,與姜黃素聯(lián)合順鉑組比較,姜黃素組和順鉑組Notch1表達(dá)明顯上調(diào),EGFR表達(dá)則明顯下調(diào),而姜黃素聯(lián)合順鉑組對Notch1和EGFR表達(dá)調(diào)控的影響與姜黃素組和順鉑組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論姜黃素與順鉑在抑制人舌鱗癌Tca-8113細(xì)胞增殖時(shí)具有協(xié)同效應(yīng),其機(jī)制可能與上調(diào)Notch1信號通路、下調(diào)EGFR信號通路有關(guān)。
[Abstract]:Objective To observe the effect of curcumin or curcumin combined with cisplatin on human tongue squamous cell carcinoma (Tca-8113) cells in vitro, and to observe the effect of its effect on the proliferation of human tongue squamous cell carcinoma cells, and to explore the possible anti-tumor mechanism. Methods the cultured Tca-8113 cells were randomly divided into curcumin group, cisplatin group, curcumin combined with cisplatin group and control group.MTT method to detect each drug. The inhibitory effect on the proliferation of Tca-8113 cells in tongue squamous cell carcinoma; the effect of Hoechst 33258 staining on the morphology of nucleus of tongue squamous cell carcinoma; flow cytometry to detect the apoptosis rate; the effect of Western blotting on the expression of Notch1 and epidermal growth factor receptor (EGFR) protein in Tca-8113 cells of tongue squamous cell carcinoma. Results MTT method junction The effect of curcumin combined with cisplatin was significantly higher than the curcumin group and cisplatin group (P0.05). The results of flow cytometry showed that the apoptosis rate of curcumin combined with cisplatin group was significantly higher than that of curcumin group and cisplatin group (P0.05).Western blotting, and the ratio of curcumin combined with cisplatin group was compared with cisplatin group (P0.05). Compared with the curcumin group and cisplatin group, the expression of Notch1 was obviously up, and the expression of EGFR was obviously down. The effect of curcumin combined with cisplatin on the expression of Notch1 and EGFR was significantly different from that of the curcumin group and cisplatin group (P0.05). Conclusion curcumin and cisplatin have synergistic effects on the proliferation of Tca-8113 cells in human tongue squamous cell carcinoma. The mechanism may be related to up regulation of Notch1 signaling pathway and down regulation of EGFR signaling pathway.
【作者單位】： 錦州醫(yī)科大學(xué)附屬第二醫(yī)院口腔頜面外科;
【分類號】：R739.86

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