本文選題：胃癌 + 食欲素A　； 參考：《寧夏醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的通過酶聯(lián)免疫吸附試驗(ELISA)及免疫組織化學(xué)染色法檢測胃癌患者血清中食欲素A(OXA)的水平及組織中食欲素1型受體(OX1R)的表達情況,探討OXA在胃癌發(fā)生、發(fā)展過程中的作用。方法1.通過酶聯(lián)免疫吸附試驗(ELISA)檢測50例胃癌患者血清和30例健康體檢者血清中食欲素A的含量,并進行統(tǒng)計學(xué)分析;2.通過免疫組織化學(xué)染色法檢測胃癌組織與正常胃黏膜組織中食欲素受體1的分布情況,并進行統(tǒng)計學(xué)分析。結(jié)果(1)胃癌患者血清OXA水平明顯高于健康體檢者,差異(P0.01)存在顯著性統(tǒng)計學(xué)意義;(2)將兩組血清標(biāo)本按年齡各分為兩組(≤65歲、65歲)。兩組血清標(biāo)本中“≤65歲”組的OXA水平均高于“65歲”組,差異(t=0.59、t=0.68,P0.05)無統(tǒng)計學(xué)意義;(3)將胃癌血清標(biāo)本按其分化程度將其分為未分化胃癌、低分化胃癌、中分化胃癌、高分化胃癌組,OXA水平在胃癌分化程度上(F=0.05,P0.05)無明顯統(tǒng)計學(xué)差異。(4)正常胃粘膜組織標(biāo)本中食欲素1型受體(OX1R)陽性表達率60%,平均染色分值(3.67±1.80);胃癌組織OX1R陽性表達率94.7%,平均染色分值(7.53±3.20),與正常組織相比,胃癌組織中OX1R陽性表達率明顯增高(P0.01),且陽性平均染色分值亦顯著高于正常組(P0.01);(5)將胃癌組織標(biāo)本按照胃癌分化程度,分為分化型胃癌及未分化型胃癌兩組,分化型胃癌OX1R陽性表達的平均染色分值為(6.86±2.87),未分化型胃癌陽性表達的平均染色分值為(9.88±2.41),與分化型胃癌相比,未分化胃癌組織染色分值顯著增高(P0.05),且所檢測到的OX1R主要位于癌細(xì)胞胞漿和胞膜上,呈淺黃色至棕黃色顆粒。結(jié)論胃癌患者血清中食欲素A水平增高,胃癌組織中OX1R表達增多,提示食欲素系統(tǒng)在胃癌的發(fā)生、發(fā)展中很可能起著促進作用。
[Abstract]:Objective to investigate the role of OXA in the carcinogenesis and development of gastric cancer by using Elisa and immunohistochemical staining to detect the level of orexin A OXA in serum and the expression of orexin type 1 receptor (OX1R) in the tissues of patients with gastric cancer. Method 1. The levels of orexin A in serum of 50 patients with gastric cancer and 30 healthy controls were detected by Elisa. The distribution of orexin receptor 1 in gastric cancer and normal gastric mucosa was detected by immunohistochemical staining. Results (1) the serum OXA level in patients with gastric cancer was significantly higher than that in healthy controls (P 0.01). The serum samples were divided into two groups according to their age (鈮，

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