本文選題：肺癌 + NCI-H細胞　； 參考：《山東醫(yī)藥》2017年45期

【摘要】：目的探討自然殺傷(NK)細胞對肺癌細胞系NCI-H292的殺傷作用及對癌細胞中β-catenin蛋白表達的影響。方法無菌采集健康人外周血,培養(yǎng)NK細胞(NK組),取培養(yǎng)14 d后的NK細胞,調整細胞濃度為5×106/m L,按1∶5的比例接種到NCI-H292細胞(NK+NCI-H292組)培養(yǎng)板共培養(yǎng)24 h,單獨培養(yǎng)NCI-H292細胞(NCIH292組)24 h。采用CCK-8法測算NK細胞對肺癌細胞系NCI-H292的殺傷活性,采用Western blotting法檢測NCIH292細胞中β-catenin蛋白的表達。結果 NK細胞對NCI-H292細胞的殺傷率為96.720%±1.081%,與NK及NCI-H292組比較,NK+NCI-H292組β-catenin表達降低(P均0.01)。結論 NK細胞對NCI-H292細胞具有殺傷力,同時能夠抑制NCI-H292細胞中β-catenin蛋白的表達。
[Abstract]:Objective to investigate the cytotoxicity of natural killer (NK) cells to lung cancer cell line NCI-H292 and the expression of 尾 -catenin in human lung cancer cells. Methods Peripheral blood samples from healthy people were collected and NK cells were cultured in NK group. The NK cells were cultured for 14 days. The concentration of NK cells was adjusted to 5 脳 10 6 / mL. The NK cells were inoculated into the NCI-H292 cell / NK NCI-H292 group at 1:5 ratio for 24 hours. The NCI-H292 cells were cultured alone in NCIH292 group for 24 hours. The cytotoxicity of NK cells to NCI-H292 was measured by CCK-8 assay, and the expression of 尾 -catenin protein in NCIH292 cells was detected by Western blotting assay. Results the killing rate of NK cells on NCI-H292 cells was 96.720% 鹵1.081%. Compared with NK and NCI-H292 groups, the expression of 尾 -catenin in NK NCI-H292 group was lower than that in NK NCI-H292 group (P < 0.01). Conclusion NK cells can kill NCI-H292 cells and inhibit the expression of 尾 -catenin in NCI-H292 cells.
【作者單位】： 河北省胸科醫(yī)院·河北省肺癌防治研究中心;河北師范大學生命科學學院;
【基金】：河北省省級重大醫(yī)學研究課題(ZD2013063)
【分類號】：R734.2

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