本文選題：食管鱗癌微轉移 + CTTNmRNA　； 參考：《蘇州大學》2015年碩士論文

【摘要】：目的:初步探索食管鱗癌患者外周血中CTTNm RNA的表達,探討其作為食管鱗癌微轉移標志物的可行性和其表達的高低對食管鱗癌患者預后的影響。方法:用實時熒光定量逆轉錄聚合酶鏈式反應的方法聯(lián)合檢測40例食管鱗癌患者手術切除的腫瘤標本和食管殘端CTTNm RNA的表達并比較二者的區(qū)別,檢測40例健康獻血員的外周血標本CTTNm RNA的表達得出正常人群的表達范圍,以此為標準檢測40例食管鱗癌患者術前外周血標本是否存在CTTNRm RNA的高表達。在17個月內隨訪觀察這40例食管鱗癌患者的生存狀態(tài)。結果:1.CTTNm RNA在食管鱗癌組織中的表達量均明顯高于食管殘端組織(P0.05)。2.25例患者的外周血標本檢測出CTTNm RNA的高表達,陽性率為62.5%。3.食管鱗癌患者外周血CTTNm RNA陽性表達的患者,在對應的癌組織中都檢測到CTTNm RNA呈高表達(P0.05),食管癌患者外周血與對應癌組織中CTTNm RNA表達具有一致性。4.食管鱗癌患者外周血CTTNm RNA表達與淋巴結轉移、浸潤程度、病理分期及預后密切相關(P0.05);與性別、年齡、煙酒史、腫瘤大小、細胞分化程度等無相關性(P0.05)。5.40例食管鱗癌患者均隨訪至2015年4月,失訪4例,死亡17例,存活19例,隨訪率90.0%,17例死亡患者中位生存期4.5個月,40例患者中位隨訪時間13個月,1年生存率62.5%。外周血CTTNm RNA陽性病人死亡15例(15/25,60.0%)。單因素COX回歸分析結果顯示腫瘤位置(RR=2.783),腫瘤大小(RR=2.727),癌細胞分化程度(RR=3.649),浸潤深度(RR=3.270),淋巴結轉移(RR=24.090),遠處轉移(RR=8.371),病理分期(RR=4.135),CTTNm RNA(RR=5.660)為預后危險因素,而年齡,性別,吸煙及飲酒等均無統(tǒng)計學意義。多因素COX回歸分析結果證明淋巴結轉移和CTTNm RNA表達是影響患者生存時間的主要危險因素。在控制了淋巴結轉移等其他因素的情況下,CTTNm RNA表達每增加一個等級患者死亡的危險性增加57%。結論:1.CTTNm RNA在食管鱗癌組織及外周血中均有特異性的高表達,這意味著CTTNm RNA在食管鱗癌的發(fā)生、發(fā)展中可能起著重要的作用。2.CTTNm RNA是有價值的食管鱗癌微轉移標志物,與病人的淋巴結轉移、浸潤程度、病理分期及預后密切相關,而與性別、年齡、腫瘤部位、大小及腫瘤細胞分化程度沒有相關性。3.外周血中CTTNm RNA的表達可能是影響食管鱗癌患者生存狀態(tài)的獨立危險因素。
[Abstract]:Objective: to explore the expression of CTTNm RNA in peripheral blood of patients with esophageal squamous cell carcinoma (ESCC), and to explore the feasibility of its expression as a micrometastasis marker of esophageal squamous cell carcinoma (ESCC) and the influence of its expression on the prognosis of esophageal squamous cell carcinoma (ESCC) patients. Methods: real-time fluorescence quantitative reverse transcriptase polymerase chain reaction was used to detect the expression of CTTNm RNA in 40 patients with esophageal squamous cell carcinoma. The expression of CTTNm RNA in peripheral blood samples of 40 healthy blood donors was detected to determine the range of expression of CTTNRm RNA in normal subjects, and the high expression of CTTNRm RNA in peripheral blood samples of 40 patients with esophageal squamous cell carcinoma (ESCC) before operation was detected. The survival status of 40 patients with esophageal squamous cell carcinoma was observed during 17 months follow-up. Results 1. The expression of CTTNm RNA in esophageal squamous cell carcinoma was significantly higher than that in esophageal stump tissue P0.05. 2.25 patients' peripheral blood samples showed high expression of CTTNm RNA, and the positive rate was 62.5 and 3.The expression of CTTNm in esophageal squamous cell carcinoma was significantly higher than that in esophageal stump tissue. The positive expression of CTTNm RNA in peripheral blood of patients with esophageal squamous cell carcinoma was significantly higher than that in corresponding cancer tissues. The expression of CTTNm RNA in peripheral blood of esophageal cancer patients was consistent with that of corresponding cancer tissues. The expression of CTTNm RNA in peripheral blood of patients with esophageal squamous cell carcinoma was closely related to lymph node metastasis, degree of invasion, pathological stage and prognosis (P 0.05), and was associated with sex, age, history of tobacco and alcohol, tumor size, and so on. All patients with esophageal squamous cell carcinoma were followed up until April 2015. 4 cases were lost, 17 cases died, 19 cases survived. The median survival time of 17 patients with death was 4.5 months and the median follow-up time of 40 patients was 13 months. The 1-year survival rate was 62.5. 15 patients died of CTTNm RNA positive in peripheral blood. The results of univariate COX regression analysis showed that the location of the tumor was 2.783%, the size of the tumor was 2.727%, the degree of differentiation of cancer cells was 3.649%, the depth of invasion was 3.270%, the lymph node metastasis was 24.090%, the distant metastasis was 8.371C, the pathological stage was RR4.135% CTTNM RNA-RNA 5.660) as the prognostic risk factors, age and sex. Smoking and drinking were not statistically significant. Multivariate COX regression analysis showed that lymph node metastasis and CTTNm RNA expression were the main risk factors for survival time. After controlling for other factors such as lymph node metastasis, the risk of death for each additional grade of CTTNm RNA was increased by 57%. Conclusion: 1. CTTNM RNA is highly expressed in esophageal squamous cell carcinoma tissues and peripheral blood, which means that CTTNm RNA may play an important role in the occurrence and development of esophageal squamous cell carcinoma. 2. CTTNm RNA is a valuable micrometastasis marker of esophageal squamous cell carcinoma. It was closely related to lymph node metastasis, degree of invasion, pathological stage and prognosis, but had no correlation with sex, age, tumor location, size and differentiation degree of tumor cells. The expression of CTTNm RNA in peripheral blood may be an independent risk factor for the survival status of esophageal squamous cell carcinoma patients.
【學位授予單位】：蘇州大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R735.1

【參考文獻】

相關期刊論文 前1條

1 ;An analysis of esophageal cancer incidence in Cixian county from 1974 to 1996[J];World Journal of Gastroenterology;2003年02期

，

本文編號：1959946