本文選題：肺癌 + 復(fù)制蛋白變體��； 參考：《南京大學(xué)》2015年碩士論文

【摘要】：肺癌作為發(fā)病率和死亡率增長(zhǎng)最快的惡性腫瘤之一,是人類健康的一大殺手。近年來環(huán)境惡化,空氣污染日益嚴(yán)重,加上一直以來居高不下的吸煙率,肺癌的發(fā)病率正呈明顯上升趨勢(shì)。早期肺癌由于癥狀不明顯,加上傳統(tǒng)的篩查方法操作復(fù)雜,很容易被忽視,因此肺癌的早期檢測(cè)成為一個(gè)迫切需要解決的問題。本實(shí)驗(yàn)初步建立了人復(fù)制蛋白變體(CIZ1-V)的膠乳增強(qiáng)免疫比濁檢測(cè)方法,用于早期肺癌的檢測(cè)。用制備的CIZ1-V多克隆抗體(兔抗)與0.13μm粒徑的膠乳微球顆粒進(jìn)行化學(xué)偶聯(lián),研制CIZ1-V試劑盒,并對(duì)試劑盒性能進(jìn)行鑒定。結(jié)果表明,CIZ1-V試劑盒標(biāo)準(zhǔn)曲線呈線型,相關(guān)系數(shù)R2=0.9988；檢測(cè)范圍為0.20～8.00 gg/mL,靈敏度為0.11μg/mL,檢測(cè)限為0.20μg/mL。試劑盒對(duì)表達(dá)純化所得目的蛋白的回收率范圍為98.92%～104.00%,平均100.91%；平均批內(nèi)及批間變異系數(shù)均小于5%,符合國家檢測(cè)試劑盒標(biāo)準(zhǔn)。試劑盒和Elisa方法對(duì)于表達(dá)純化所得目的蛋白的回收率無顯著差異(P≥0.05),對(duì)1L菌液的CIZ1-V表達(dá)量測(cè)定結(jié)果也無顯著差異(P≥0.05)。本實(shí)驗(yàn)初步研制出敏感、準(zhǔn)確、特異的CIZ1-V檢測(cè)試劑盒,為早期肺癌的血清中CIZ1-V的檢測(cè)打下了基礎(chǔ)。
[Abstract]:Lung cancer, one of the fastest growing malignant tumors, is a major killer of human health. In recent years, the incidence of lung cancer is on the rise due to the deterioration of the environment, the increasing air pollution and the high smoking rate. Because the symptoms of early lung cancer are not obvious, and the traditional screening method is complex, it is easy to be ignored, so the early detection of lung cancer becomes an urgent problem to be solved. In this study, a method of latex enhanced immune turbidimetry (RIA) was established for detection of early lung cancer. The CIZ1-V polyclonal antibody (rabbit antibody) was chemically coupled with 0.13 渭 m latex microspheres to prepare the CIZ1-V kit, and the properties of the kit were evaluated. The results showed that the standard curve of CIZ1-V kit was linear, the correlation coefficient was 0.9988, the detection range was 0.20 ~ 8.00 gg-mL, the sensitivity was 0.11 渭 g / mL, the detection limit was 0.20 渭 g / mL. The recovery range of the purified protein was 98.92%, the average value was 100.91.The average coefficient of variation within and between batches was less than 5, which was in line with the national detection kit standard. There was no significant difference between the kit and Elisa method in the recovery of the purified protein (P 鈮，

本文編號(hào)：1940066