本文選題：結(jié)腸癌 + 上皮間質(zhì)轉(zhuǎn)化��； 參考：《中國腫瘤臨床》2017年10期

【摘要】：目的:研究p-STAT3的活化在結(jié)腸癌中的表達(dá)與Snail、MMP2的相關(guān)性,及其在離體細(xì)胞實驗中激活受抑后對結(jié)腸癌細(xì)胞遷移能力的影響并探討其機(jī)制。方法:免疫組織化學(xué)染色檢測p-STAT3與Snail、MMP2的表達(dá)及其相關(guān)性,分析三者與TNM分期、遠(yuǎn)處轉(zhuǎn)移、淋巴結(jié)轉(zhuǎn)移及分化程度之間的關(guān)系;MTT實驗篩選AG490對增殖無影響的濃度和時間,并用該濃度和時間進(jìn)行后續(xù)實驗;采用Western blot法檢測AG490抑制p-STAT3活化后STAT3、Snail、MMP2蛋白表達(dá)情況;劃痕實驗觀察p-STAT3活化受抑后,結(jié)腸癌細(xì)胞遷移情況。結(jié)果:免疫組織化學(xué)染色結(jié)果表明,p-STAT3的表達(dá)與Snail、MMP2均存在相關(guān)性,且均與淋巴結(jié)轉(zhuǎn)移相關(guān)(P0.05)。在兩個結(jié)腸癌細(xì)胞系中,通過MTT實驗,選出10μM作為AG490的最佳作用濃度,并采用該濃度進(jìn)行后續(xù)實驗。AG490抑制p-STAT3活化后Snail、MMP2表達(dá)明顯下降,而STAT3總蛋白表達(dá)無明顯變化。且當(dāng)AG490抑制p-STAT3活化后,細(xì)胞的遷移能力明顯下降。結(jié)論:p-STAT3可以通過上皮間質(zhì)轉(zhuǎn)化(epithelial-mesenchymal transition,EMT)促進(jìn)結(jié)腸癌的轉(zhuǎn)移。
[Abstract]:Aim: to study the relationship between the expression of p-STAT3 activation in colon cancer and Snailan MMP2, and its effect on the migration ability of colon cancer cells after activation and inhibition in vitro. Methods: immunohistochemical staining was used to detect the expression of p-STAT3 and Snailman MMP2 and their correlation with TNM staging, distant metastasis, lymph node metastasis and differentiation were analyzed. Western blot assay was used to detect the expression of STAT3SnailmMP2 protein after p-STAT3 activation, and scratch test was used to observe the migration of colon cancer cells after p-STAT3 activation was inhibited. Results: the results of immunohistochemical staining showed that the expression of p-STAT3 was correlated with the expression of SnailmMP2, and the expression of p-STAT3 was associated with lymph node metastasis (P0.05). In two colon cancer cell lines, 10 渭 M was selected as the best concentration of AG490 by MTT assay. The following experiment. AG490 was used to inhibit the expression of Snailan MMP2 after p-STAT3 activation, but the total protein expression of STAT3 did not change significantly. When AG490 inhibited the activation of p-STAT3, the migration ability of the cells decreased significantly. Conclusion:% p-STAT3 can promote the metastasis of colon cancer through epithelial-mesenchymal transition.
【作者單位】： 天津醫(yī)科大學(xué)病理學(xué)教研室;天津醫(yī)科大學(xué)腫瘤醫(yī)院病理科;天津醫(yī)科大學(xué)總醫(yī)院病理科;
【基金】：國家自然科學(xué)基金面上項目(編號:81572872);國家自然科學(xué)基金重點(diǎn)項目(編號:81230050)資助~~
【分類號】：R735.35

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