本文選題：LPA1-3 + 姜黃素��； 參考：《蘭州大學》2017年碩士論文

【摘要】：目的:1.探究正常胃黏膜上皮組織、高分化胃腺癌、中分化胃腺癌、低分化胃腺癌中LPA1-3的表達差異水平。2.探究姜黃素(curcumin)對正常胃上皮細胞(GES-1)、高分化胃腺癌細胞(MKN28)、中分化胃腺癌細胞(SGC-7901)及低分化胃腺癌細胞(MKN45)體外增殖的抑制,細胞凋亡的影響以及遷移侵襲能力的影響。方法:1.使用免疫組織化學染色S-P法觀察LPA1-3在正常胃上皮及高中低分化胃腺癌組織中表達水平的差異。2.體外培養(yǎng)正常胃黏膜上皮細胞(GES-1)、高分化胃腺癌細胞(MKN28)、中分化胃腺癌細胞(SGC-7901)及低分化胃腺癌細胞(MKN45)。3.Western Blot檢測細胞株中LPA1-3表達水平。4.MTT比色法檢測姜黃素對四種細胞體外增殖的抑制。5.Hoechst33342染色觀察細胞凋亡。6.Transwell小室觀察姜黃素對四種細胞遷移侵襲能力的影響。7.應用Imagepro Plus 6.0軟件對免疫組織化學染色結(jié)果進行分析,所有數(shù)據(jù)使用SPSS20.0進行分析。結(jié)果:1.免疫組化染色結(jié)果顯示:(1).正常組、高中低分化胃腺癌組中LPA2表達水平均高于LPA1與LPA3(p≤0.05),LPA1與LPA3表達無顯著差異(p0.05);(2).LPA1在正常組、高中低胃腺癌組中表達無顯著差異(p0.05);LPA2在中分化組中表達低于正常組、高分化組及低分化組(p≤0.05);LPA3在高中低胃腺癌組中表達均低于正常組(p≤0.05),但在高中低胃腺癌組中表達無顯著差異(p0.05)。2.各濃度的姜黃素均可抑制GES-1、MKN28、SGC-7901、MKN45四種細胞的增殖,與對照組對比均有顯著差異(p≤0.05),且具有時間劑量依賴性。3.姜黃素可誘導GES-1、MKN28、SGC-7901、MKN45四種細胞凋亡的發(fā)生。4.姜黃素在低濃度(20μmol/L和40μmol/L)時可促進GES-1細胞的遷移及侵襲能力,在高濃度(80μmol/L)時抑制;姜黃素可抑制MKN28、SGC-7901、MKN45三種細胞遷移及侵襲能力,且呈劑量依賴性。5.Western Blot結(jié)果顯示:在GES-1細胞中,LPA1高于LPA2和LPA3(p≤0.05),MKN28和SGC-7901細胞中,LPA2均高于LPA1與LPA3(p≤0.05),MKN45細胞中,LPA3高于LPA1與LPA2(p≤0.05)。結(jié)論:1.LPA2在正常胃上皮組織、高、中、低分化胃腺癌組織中的表達均高于LPA1與LPA3,在α=0.05水平具有顯著差異。2.姜黃素可抑制GES-1、MKN28、SGC-7901、MKN45四種細胞的增殖,并誘導凋亡的發(fā)生。3.低濃度姜黃素會促進GES-1細胞遷移及侵襲,在高濃度時抑制;姜黃素對高中低分化胃腺癌細胞遷移及侵襲能力有抑制作用。
[Abstract]:Purpose 1. To investigate the differential expression of LPA1-3 in normal gastric mucosal epithelium, well differentiated gastric adenocarcinoma, moderately differentiated gastric adenocarcinoma and poorly differentiated gastric adenocarcinoma. To investigate the inhibitory effect of curcumin on proliferation, apoptosis and migration and invasion of normal gastric epithelial cells, well-differentiated gastric adenocarcinoma cell line MKN28, moderately differentiated gastric adenocarcinoma cell line SGC-7901) and poorly differentiated gastric adenocarcinoma cell line MKN45) in vitro. Method 1: 1. Immunohistochemical staining S-P method was used to observe the expression of LPA1-3 in normal gastric epithelium and poorly differentiated gastric adenocarcinoma. Normal gastric mucosal epithelial cells were cultured in vitro, well differentiated gastric adenocarcinoma cell line MKN28, moderately differentiated gastric adenocarcinoma cell line SGC-7901) and poorly differentiated gastric adenocarcinoma cell line MKN45. 3. Western Blot were used to detect the expression level of LPA1-3 in cell line. 4. MTT colorimetric assay was used to detect curcumin on four kinds of cells in vitro. Cell apoptosis was observed by Hoechst33342 staining. 6. Transwell chamber was used to observe the effect of curcumin on the migration and invasion of four kinds of cells. The results of immunohistochemical staining were analyzed by Imagepro Plus 6.0 software, and all data were analyzed by SPSS20.0. The result is 1: 1. The results of immunohistochemical staining showed that: 1. There was no significant difference in the expression of LPA2 between LPA1 and LPA3(p 鈮，

本文編號：1928104