發(fā)布時間：2018-05-18 05:30

  本文選題：胰腺腫瘤 + GPSM2��； 參考：《江蘇大學》2017年碩士論文

【摘要】：研究目的:本實驗通過構建G蛋白信號調節(jié)蛋白2(G-p roteinsignaingmodulator2,GPSM2)穩(wěn)定高表達的胰腺癌細胞株,研究GPSM2與人胰腺癌細胞遷移能力的關系。探討以GPSM2作分子靶點,為胰腺癌的治療提供新思路和新策略。研究方法:1、構建GPSM2基因過表達質粒,并轉染人胰腺癌MIA-PaCa-2細胞株。2、采用RT-PCR技術檢測GPSM2基因轉染組、未轉染組(空白對照組)和空載體p C M V-Tag3B轉染組(陰性對照組)中GPSM2基因m R N A的表達水平。3、Western-blot檢測各組胰腺癌細胞GPSM2、β-連環(huán)蛋白(β-catenin)的表達。4、采用Transwell實驗檢測各組胰腺癌細胞遷移能力變化。研究結果:1、成功構建了GPSM2穩(wěn)定高表達的重組細胞株。2、RT-PCR結果顯示GPSM2基因轉染組的m R N A表達量較陰性對照組及空白對照組明顯增加(均P0.0 1),與空白對照組相比,GPSM2基因上調效率達到了7 3.3倍。陰性對照組與空白對照組間GPSM2mRNA表達水平差異無統(tǒng)計學意義(P0.0 5)3、Western-blot結果證實GPSM2基因重組細胞株中GPSM2蛋白、β-catenin蛋白均高表達(均P0.0 5)。陰性對照組與空白對照組間GPSM2蛋白及β-catenin蛋白表達水平差異無統(tǒng)計學意義(均P0.0 5)。采用一元線性回歸分析得出胰腺癌細胞中GPSM2與β-catenin的表達水平呈正比(P0.0 5)。4、Transwel實驗發(fā)現(xiàn)GPSM2穩(wěn)定高表達的胰腺癌細胞株的遷移細胞計數明顯高于陰性對照組及空白對照組(均P0.0 1)。結論:GPSM2穩(wěn)定高表達的胰腺癌細胞株的遷移細胞計數明顯增加,在人胰腺癌細胞中上調GPSM2的表達能使β-catenin蛋白高表達,可能由此促進人胰腺癌細胞的遷移能力。
[Abstract]:Objective: to study the relationship between GPSM2 and the migration ability of human pancreatic cancer cells by constructing a stable high expression pancreatic cancer cell line 2(G-p rotein signal modulator 2 (GPSM2). To explore the use of GPSM2 as a molecular target to provide new ideas and strategies for the treatment of pancreatic cancer. Methods: GPSM2 gene overexpression plasmid was constructed and transfected into human pancreatic cancer MIA-PaCa-2 cell line. The RT-PCR technique was used to detect the GPSM2 gene transfection group. The expression level of GPSM2 gene m R N A in untransfected group (blank control group) and empty vector p C M V-Tag3B transfection group (negative control group). The expression of GPSM2 gene m R N A in pancreatic cancer cells of each group was detected by Western blot. The expression of GPSM2 and 尾 -catenin was detected by Transwell assay. The migration ability of pancreatic cancer cells in group B was changed. The results showed that the expression of m R N A in the transfected GPSM2 gene group was significantly higher than that in the negative control group and the blank control group (all P0.01, compared with the blank control group). The results of RT-PCR showed that the expression of m R N A in the GPSM2 gene transfected group was significantly higher than that in the negative control group and the blank control group (all P0.01, compared with the blank control group). The efficiency of gene up-regulation was 73.3 times. There was no significant difference in the expression of GPSM2mRNA between the negative control group and the blank control group. The results of Western-blot showed that the expression of GPSM2 protein and 尾 -catenin protein in the recombinant cell line of GPSM2 gene were all high (all P0.05). There was no significant difference in the expression of GPSM2 protein and 尾 -catenin protein between the negative control group and the blank control group (P 0.05). Univariate linear regression analysis showed that the expression of GPSM2 and 尾 -catenin in pancreatic cancer cells was directly proportional to that of P0.05N. 4. Transwell assay showed that the number of migrating cells in pancreatic cancer cell lines with stable and high GPSM2 expression was significantly higher than that in negative control group and blank control group (all P 0.01). Conclusion the migration cell count of human pancreatic cancer cell lines with stable and high expression of GPSM2 increased significantly. Upregulating the expression of GPSM2 in human pancreatic cancer cells may lead to the overexpression of 尾 -catenin protein, which may promote the migration ability of human pancreatic cancer cells.
【學位授予單位】：江蘇大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R735.9

【參考文獻】

相關期刊論文 前7條

1 Chiara Caparello;Laura L Meijer;Ingrid Garajova;Alfredo Falcone;Tessa Y Le Large;Niccola Funel;Geert Kazemier;Godefridus J Peters;Enrico Vasile;Elisa Giovannetti;;FOLFIRINOX and translational studies: Towards personalized therapy in pancreatic cancer[J];World Journal of Gastroenterology;2016年31期

2 彭云;崔磊;史堅強;陳吉祥;瞿建國;黨勝春;張建新;;G蛋白信號調節(jié)蛋白2在胰腺癌組織中的表達及臨床意義[J];江蘇大學學報(醫(yī)學版);2016年03期

3 朱建偉;熊力;馬望;樊青霞;張亞娜;文宇;苗雄鷹;;胰腺癌干細胞研究進展[J];中國普通外科雜志;2015年09期

4 秦毅;梁丁孔;施思;吉順榮;張波;許文彥;劉江;徐近;倪泉興;虞先o，

本文編號：1904591