本文選題：乳腺癌 + 號(hào)染色體�。� 參考：《中華腫瘤防治雜志》2017年12期

【摘要】：目的乳腺癌是影響婦女健康的主要癌癥之一,且發(fā)病率呈現(xiàn)明顯上升趨勢(shì)。DNA拷貝數(shù)變化(copy number variation,CNV)能夠影響基因表達(dá),從而引起腫瘤發(fā)生,研究CNV對(duì)探索腫瘤的發(fā)病機(jī)制有很大幫助。本研究探討乳腺癌17號(hào)染色體拷貝數(shù)變異和臨床病理特征關(guān)系。方法應(yīng)用熒光原位雜交(fluorescence in situ hybridization,FISH)法檢測(cè)2010-01-01-2011-01-01新疆醫(yī)科大學(xué)附屬腫瘤醫(yī)院乳腺潤(rùn)性導(dǎo)管癌標(biāo)本283例,觀察17號(hào)染色體拷貝數(shù)情況及人表皮生長(zhǎng)因子受體2(human epidermal growth factor receptor 2,HER2)基因擴(kuò)增情況,并用免疫組織化學(xué)法檢測(cè)HER2受體蛋白的表達(dá)水平。結(jié)果 283例乳腺癌中17號(hào)染色體拷貝數(shù)多倍體為43例(15.19%),雙倍體為226例(79.86%),單倍體為14例(4.95%),乳腺癌17號(hào)染色體多倍體HER2基因陽(yáng)性率為53.49%(23/43)。283例乳腺癌中HER2基因表達(dá)陽(yáng)性為84例,陽(yáng)性率為29.68%(84/283);17號(hào)染色體拷貝數(shù)不同在HER2基因是否擴(kuò)增方面差異有統(tǒng)計(jì)學(xué)意義,χ~2=9.564,P=0.007;乳腺癌17號(hào)染色體拷貝數(shù)變異在p53基因表達(dá)(χ~2=8.181,P=0.017)、病理組織學(xué)分級(jí)(χ~2=11.203,P=0.019)方面差異有統(tǒng)計(jì)學(xué)意義,與年齡、腫瘤大小和淋巴結(jié)轉(zhuǎn)移等指標(biāo)差異無(wú)統(tǒng)計(jì)學(xué)意義。17號(hào)染色體拷貝數(shù)不同組中預(yù)后存在差異,差異有統(tǒng)計(jì)學(xué)意義,χ~2=241.363,P=0.001。結(jié)論 17號(hào)染色體拷貝數(shù)變異和乳腺癌病理特征及預(yù)后相關(guān),檢測(cè)乳腺癌中17號(hào)染色體拷貝數(shù)變異可作為指導(dǎo)臨床治療和判斷預(yù)后的參考指標(biāo)。
[Abstract]:Objective Breast cancer is one of the major cancers affecting women's health, and the incidence of breast cancer is obviously increasing. Copy number variation can affect gene expression and cause tumorigenesis. The study of CNV is of great help in exploring the pathogenesis of tumor. This study was to investigate the relationship between chromosome 17 copy number variation and clinicopathological features of breast cancer. Methods fluorescence in situ hybridization (fish) method was used to detect 283 breast ductal carcinoma samples from Xinjiang Medical University affiliated Cancer Hospital from January to January 2011. The copy number of chromosome 17 and the amplification of human epidermal growth factor receptor 2(human epidermal growth factor receptor 2HER2 gene were observed. The expression of HER2 receptor protein was detected by immunohistochemistry. Results among 283 cases of breast cancer, 43 cases were polyploid of chromosome 17 copy number, 226 cases were diploidy, 14 cases were haploid, and the positive rate of polyploid HER2 gene on chromosome 17 was 53.49%. The positive rate of HER2 gene was 84 cases in 23 / 43 / 283 cases of breast cancer. The positive rate of chromosome 17 was 29.68% / 283%; the number of copies of chromosome 17 was significantly different in the amplification of HER2 gene, 蠂 ~ 2 / 9.564 / P ~ (0.007); the variation of chromosome 17 copy number of breast cancer was significantly different in the expression of p53 gene (蠂 ~ (2) (蠂 ~ (2) = 8.181), histopathological grade (蠂 ~ (211.203) P ~ (0.019). There was no significant difference in tumor size and lymph node metastasis, but there was significant difference in prognosis among the groups with different copy number of chromosome 17, 蠂 ~ (2 +) 241.363 (P ~ (0.001). Conclusion the variation of chromosome 17 copy number is related to the pathological characteristics and prognosis of breast cancer. Detection of variation of chromosome 17 copy number in breast cancer can be used as a reference index for clinical treatment and prognosis.
【作者單位】： 新疆醫(yī)科大學(xué)附屬腫瘤醫(yī)院乳腺外科一病區(qū);
【基金】：烏魯木齊市科學(xué)技術(shù)計(jì)劃(Y151310041)
【分類號(hào)】：R737.9
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本文編號(hào)：1904378