發(fā)布時(shí)間：2018-05-09 08:53

  本文選題：釕配合物 + 肝癌細(xì)胞�。� 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:在全球經(jīng)濟(jì)蓬勃發(fā)展的背景下,隨著醫(yī)療水平的逐步提高,腫瘤的發(fā)病率和檢測(cè)率同時(shí)也在逐年的提高,腫瘤仍然是當(dāng)今社會(huì)人類健康的一個(gè)重大威脅。近幾年來各國(guó)學(xué)者對(duì)于原發(fā)性肝癌的發(fā)病機(jī)制及其治療效果取得了許多重大的研究成果,但其生存率依然較低,預(yù)后仍差的現(xiàn)象一直無法取得新的進(jìn)展,因此尋求新的治療藥物與治療方法具有重要意義�；熕幬镌诋�(dāng)下治療腫瘤的研究中已經(jīng)取得很大的發(fā)展,同時(shí)也為原發(fā)性肝癌的治療提供了新的思路。諸多文獻(xiàn)均有報(bào)道,釕配合物能夠抑制腫瘤細(xì)胞的生長(zhǎng),觀察釕配合藥物對(duì)肝癌細(xì)胞生長(zhǎng)的抑制作用,從而為臨床工作提供新的治療依據(jù)。方法:實(shí)驗(yàn)室培養(yǎng)肝癌BEL-7402細(xì)胞,將培養(yǎng)的細(xì)胞隨機(jī)分為A、B兩組,A組為空白對(duì)照組,B組中加入釕配合藥物Ru-HMIP,使用MTT法測(cè)定細(xì)胞增殖的抑制率,用流式細(xì)胞術(shù)測(cè)定細(xì)胞的凋亡率。結(jié)果:1.MTT法檢測(cè)結(jié)果示:同對(duì)照組對(duì)比,釕配合藥物Ru-HMIP對(duì)肝癌細(xì)胞生長(zhǎng)具有抑制作用,抑制率大于對(duì)照組。2.流式細(xì)胞儀檢測(cè)結(jié)果顯示:釕配合藥物Ru-HMIP作用的肝癌細(xì)胞,肝癌細(xì)胞凋亡明顯增多。結(jié)論:在肝癌BEL-7402細(xì)胞生長(zhǎng)過程中,釕配合藥物對(duì)其具有抑制作用。
[Abstract]:Objective: with the rapid development of the global economy, with the gradual improvement of the medical level, the incidence and detection rate of tumor are also increasing year by year. Cancer is still a major threat to human health in today's society. In recent years, many scholars have made a lot of significant effects on the pathogenesis and treatment effect of primary liver cancer. However, the survival rate is still low and the prognosis is still poor, so it is of great significance to seek new therapeutic drugs and treatment methods. Chemotherapy drugs have made great progress in the current research of cancer treatment, and also provide new ideas for the treatment of primary liver cancer. It is reported that ruthenium complexes can inhibit the growth of tumor cells and observe the inhibitory effect of ruthenium combined with drugs on the growth of hepatoma cells, thus providing new therapeutic basis for clinical work. Methods: the BEL-7402 cells of liver cancer were cultured in laboratory. The cultured cells were randomly divided into A, B two groups, A group as blank control group, and B group joined ruthenium. The inhibitory rate of cell proliferation was measured by MTT method and the apoptosis rate of cells was measured by flow cytometry. Results: the results of 1.MTT assay showed that ruthenium combined with drug Ru-HMIP had inhibitory effect on the growth of hepatoma cells compared with the control group, and the inhibition rate was greater than that of the control group of.2. flow cytometer: ruthenium combined with drug Ru-HMIP. The apoptosis of HCC cells increased significantly. Conclusion: Ru combined with drugs can inhibit the growth of BEL-7402 cells.

【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.7

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