本文選題：鼻咽腫瘤 + 注射用黃芪多糖��； 參考：《實(shí)用醫(yī)學(xué)雜志》2017年08期

【摘要】：目的:探討注射用黃芪多糖誘導(dǎo)人鼻咽癌細(xì)胞株CNE-2的自噬作用。方法:采用CCK8檢測(cè)注射用黃芪多糖對(duì)CNE-2增殖的抑制作用;吖啶橙染色觀察注射用黃芪多糖作用于CNE-2細(xì)胞引起的自噬形態(tài)變化;透射電鏡觀察注射用黃芪多糖作用于CNE-2細(xì)胞后引起的細(xì)胞超微結(jié)構(gòu)變化。Western blot實(shí)驗(yàn)檢測(cè)注射用黃芪多糖作用于CNE-2細(xì)胞后Beclin-1的表達(dá)變化。結(jié)果:注射用黃芪多糖對(duì)CNE-2細(xì)胞的生長(zhǎng)有明顯抑制作用,且具有濃度及時(shí)間依賴性。注射用黃芪多糖作用后可誘導(dǎo)CNE-2細(xì)胞發(fā)生自噬。透射電鏡下細(xì)胞體內(nèi)大空泡及膜性雙層結(jié)構(gòu)增多;吖啶橙染色可見(jiàn)注射用黃芪多糖作用后觀察到更多的亮紅色酸性自噬泡。Western blot結(jié)果表明Beclin-1蛋白表達(dá)增高。結(jié)論:注射用黃芪多糖抑制細(xì)胞增殖,可能通過(guò)上調(diào)beclin1表達(dá)誘導(dǎo)人鼻咽癌細(xì)胞CNE-2細(xì)胞發(fā)生自噬。
[Abstract]:Objective: To investigate the autophagy effect of injection of Astragalus Polysaccharides on human nasopharyngeal carcinoma cell line CNE-2. Methods: CCK8 was used to detect the inhibitory effect of Astragalus Polysaccharides on CNE-2 proliferation; acridine orange staining was used to observe the changes of autophagy induced by Astragalus Polysaccharide on CNE-2 cells. The transmission electron microscope was used to observe the effect of Astragalus Polysaccharide on CNE-2 The ultrastructural change of cells after the cell.Western blot test was used to detect the expression of Beclin-1 in CNE-2 cells after injection of Astragalus polysaccharides. Results: the injection of Astragalus Polysaccharides had obvious inhibitory effect on the growth of CNE-2 cells, and was dependent on the concentration and time. The injection of Astragalus polysaccharides could induce the occurrence of CNE-2 cells. Autophagy. Under transmission electron microscope, the large vacuoles and membranous double layer structures in the cells in the cells increased; acridine orange staining showed that more bright red acid autophagic vesicles.Western blot results showed that the expression of Beclin-1 protein increased after injection of Astragalus polysaccharides. Conclusion: injection of Astragalus polysaccharide to inhibit cell proliferation may be induced by up regulation of Beclin1 expression. Autophagy occurs in the CNE-2 cells of human nasopharyngeal carcinoma cells.

【作者單位】： 柳州市人民醫(yī)院;南方醫(yī)科大學(xué)珠江醫(yī)院腫瘤中心;
【基金】：柳州市人民醫(yī)院院內(nèi)立項(xiàng)項(xiàng)目(編號(hào):lry201506)
【分類號(hào)】：R739.63

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本文編號(hào)：1864401