本文選題：視黃酸早期轉(zhuǎn)錄因子ε + 髓系抑制性細(xì)胞��； 參考：《中國腫瘤生物治療雜志》2017年07期

【摘要】：目的:探討表達(dá)小鼠NKG2D配體之一視黃酸早期轉(zhuǎn)錄因子1ε(retinoic acid early transcript 1ε,RAE1ε)的原B淋巴細(xì)胞Ba F3對乳腺癌細(xì)胞株4T1成瘤小鼠來源的髓系抑制性細(xì)胞(myeloid-derived suppressor cell,MDSC)功能的影響。方法:以小鼠原B淋巴細(xì)胞株Ba F3為基礎(chǔ),構(gòu)建表達(dá)RAE1ε的Ba F3-RAE1ε細(xì)胞以及表達(dá)空質(zhì)粒的Ba F3-mock對照細(xì)胞。通過4T1原位腫瘤模型誘導(dǎo)產(chǎn)生CD11b+Gr-1+MDSC,將Ba F3-mock細(xì)胞和Ba F3-RAE1ε細(xì)胞作為刺激細(xì)胞,分別與脾MDSC共培養(yǎng),流式細(xì)胞術(shù)檢測MDSC表面CD40、CD80、F4/80、CD11c的表達(dá)和MDSC內(nèi)活性氧(ROS)的水平;ELISA法檢測共培養(yǎng)上清液IL-10、IL-4和IFN-γ的含量;Griess法檢測共培養(yǎng)上清液一氮化氮(NO)的濃度。磁珠分選共培養(yǎng)體系中的MDSC,檢測裂解后精氨酸酶的活性;另外,將分選后的MDSC與抗CD3/抗CD28抗體活化的脾細(xì)胞共培養(yǎng),CFSE法檢測活化的CD3+CD8+T細(xì)胞增殖情況。結(jié)果:成功獲得4T1原位腫瘤模型來源的小鼠脾MDSC。與Ba F3-mock細(xì)胞相比,Ba F3-RAE1ε細(xì)胞刺激對MDSC分泌IL-4、IFN-γ、IL-10和NO的水平?jīng)]有明顯影響(P0.05);對MDSC表達(dá)CD40、CD80、F4/80、CD11c和ROS也沒有顯著影響(P0.05)。與Ba F3-mock細(xì)胞相比,Ba F3-RAE1ε細(xì)胞刺激顯著提高M(jìn)DSC的精氨酸酶活性(156.166±1.209 vs 110.135±7.356,P0.01),并明顯增強(qiáng)MDSC對CD8+T細(xì)胞增殖的抑制作用。結(jié)論:RAE-1ε在體外增強(qiáng)4T1成瘤小鼠來源的MDSC的抑制功能。
[Abstract]:Aim: to investigate the effect of Baf3, one of the mouse NKG2D ligands, on the function of myeloid-derived suppressor cell line (MDSCC) derived from mouse breast cancer cell line 4T1, and to investigate the effect of BaF3 on the function of the proto-B lymphocytes expressing retinoic acid early transcript 1 蔚 -RAE1 蔚. Methods: Ba F3-RAE1 蔚 cells expressing RAE1 蔚 and Ba F3-mock control cells expressing empty plasmids were constructed on the basis of mouse B lymphocyte line BaF3. CD11b Gr-1 MDSC was induced by 4T1 in situ tumor model. Ba F3-mock cells and Ba F3-RAE1 蔚 cells were used as stimulating cells and co-cultured with splenic MDSC, respectively. Flow cytometry was used to detect the expression of CD40, CD80, F4 / 80, CD11c on the surface of MDSC and the level of reactive oxygen species (Ros) in MDSC. The levels of IL-10, IL-4 and IFN- 緯 in co-cultured supernatants were detected by Elisa. The activity of arginase was detected by magnetic bead sorting co-culture system, and the proliferation of activated CD3 CD8 T cells was detected by co-culture of separated MDSC and spleen cells activated by anti CD3/ anti CD28 antibody. Results: the mouse spleen derived from 4T1 in situ tumor model was successfully obtained. Compared with Ba F3-mock cells, the stimulation of F3-RAE1 蔚 cells had no significant effect on the levels of IL-10 and no secreted by MDSC, nor on the expression of CD40 / CD80F4 / 80 / CD11c and ROS in MDSC. Compared with Ba F3-mock cells, the arginase activity of MDSC was significantly increased by the stimulation of Ba F3-RAE1 蔚 cells (156.166 鹵1.209 vs 110.135 鹵7.356p 0.01), and the inhibitory effect of MDSC on the proliferation of CD8 T cells was significantly enhanced. Conclusion in vitro, the inhibitory function of MDSC derived from 4T1 tumorigenic mice is enhanced by 1: RAE-1 蔚.
【作者單位】： 揚(yáng)州大學(xué)醫(yī)學(xué)院病原生物學(xué)與免疫學(xué)教研室;
【基金】：國家自然科學(xué)基金資助項目(No.81373130,No.81001308) 江蘇省自然科學(xué)基金資助項目(No.BK2010315) 揚(yáng)州大學(xué)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃資助項目~~
【分類號】：R737.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 劉秋燕;曹雪濤;;MDSCs與腫瘤免疫逃逸[J];中國腫瘤生物治療雜志;2009年04期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 錢莉;劉陽;葉楓;劉露;王少卿;賈筱琴;傅奕;龔衛(wèi)娟;田芳;;NKG2D配體RAE1ε對乳腺癌細(xì)胞4T1衍生MDSC功能的影響[J];中國腫瘤生物治療雜志;2017年07期

2 陳虹燕;郭紅;;MDSCs與腫瘤微環(huán)境[J];免疫學(xué)雜志;2015年11期

3 張朋;劉苓霜;;中醫(yī)藥調(diào)節(jié)肺癌免疫機(jī)制研究進(jìn)展[J];上海中醫(yī)藥大學(xué)學(xué)報;2015年05期

4 解雷;黃錦龍;劉淑鵬;于勇;王慧;陳培戰(zhàn);張柏和;李斌;姜小清;;替吉奧及MDSCs靶向干預(yù)劑對膽囊癌腫瘤微環(huán)境的影響[J];中華肝膽外科雜志;2015年05期

5 刁建軍;宋學(xué)東;楊雪;王清松;羅春麗;張堯;吳小候;;膀胱癌MB49細(xì)胞exosomes對MDSCs增殖及活性的影響[J];免疫學(xué)雜志;2015年02期

6 刁建軍;楊雪;宋學(xué)東;陳仕友;王胤;王曉蓉;羅春麗;張堯;吳小候;;荷瘤小鼠脾臟髓源抑制細(xì)胞的分選及鑒定[J];免疫學(xué)雜志;2014年12期

7 潘偉;沈玉娟;劉華;胡媛;姜巖巖;周何軍;徐馀信;袁忠英;王燕娟;曹建平;;髓源抑制性細(xì)胞在日本血吸蟲感染小鼠脾臟及外周血富集的研究[J];中國寄生蟲學(xué)與寄生蟲病雜志;2014年01期

8 劉紅梅;王翎;張光波;陳思文;潘旭東;毛燕青;;共刺激分子B7-H1對小鼠髓源性抑制細(xì)胞免疫抑制功能的調(diào)節(jié)作用[J];中華老年醫(yī)學(xué)雜志;2014年02期

9 符一寧;王士勇;馬克驥;劉暢;焦雪;劉迪杰;王佳玲;張暉;張哲;杜微麗;張璐;武秀艷;何英;梁春艷;劉殊;;非小細(xì)胞肺癌患者外周血髓樣抑制細(xì)胞比例及其對CD8~+T細(xì)胞抑制作用的測定[J];現(xiàn)代腫瘤醫(yī)學(xué);2013年10期

10 范舒萍;王艷紅;趙永華;米洋;侯星生;王姝月;鄧志華;羅旭光;王桂琴;;髓樣抑制細(xì)胞在自身免疫性肝炎小鼠模型中的變化及其意義[J];免疫學(xué)雜志;2013年05期

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