發(fā)布時間：2018-05-05 10:41

  本文選題：膀胱癌 + EYA4��； 參考：《安徽醫(yī)科大學》2017年碩士論文

【摘要】：目的:探討膀胱癌細胞系和膀胱移行細胞癌組織中EYA4(Eyes Absent 4:EYA轉(zhuǎn)錄共激活磷酸酶4)基因啟動子區(qū)CpG島甲基化狀態(tài)及在臨床病理特征中的意義。方法:采取甲基化特異性聚合酶鏈反應(yīng)(MSP)檢測EYA4基因在5株膀胱癌細胞系、1株膀胱永生化上皮細胞系和75例膀胱移行細胞癌組織及癌旁組織中甲基化狀態(tài),并結(jié)合臨床病理特征行統(tǒng)計學分析。結(jié)果:EYA4基因啟動子區(qū)在5株膀胱癌細胞系中,有4株出現(xiàn)甲基化,其甲基化率為80%;在人正常膀胱細胞系中甲基化陰性。EYA4在膀胱癌組織中的甲基化率為70.7%(53/75),癌旁組織中為24%(18/75),差異較為顯著,有統(tǒng)計學意義(c2=32.760,P0.01)。EYA4基因啟動子區(qū)CpG島甲基化在臨床病理特征中如單發(fā)膀胱腫瘤與多發(fā)膀胱腫瘤相比,直徑≤3cm的膀胱癌腫瘤與(29)3cm的膀胱癌腫瘤相比,低級別膀胱腫瘤與高級別膀胱腫瘤相比,淺表性膀胱腫瘤與浸潤性膀胱腫瘤相比,差異均有統(tǒng)計學意義(P0.05),但是EYA4基因啟動子區(qū)CpG島甲基化程度在與膀胱癌患者的年齡和性別之間差異不明顯,無統(tǒng)計學意義(P0.05)。結(jié)論:EYA4基因啟動子區(qū)CpG島在膀胱癌細胞系和膀胱癌組織中均表現(xiàn)為高度甲基化,推測其可能與膀胱癌的發(fā)生和發(fā)展有一定的關(guān)系,將有助于膀胱癌的早期診斷。EYA4基因啟動子區(qū)CpG島區(qū)甲基化率與膀胱癌患者的臨床病理資料如腫瘤的數(shù)目、大小、分級和分期密切相關(guān),與其年齡和性別無相關(guān)性。
[Abstract]:Objective: to investigate the methylation status of CpG island in the promoter region of EYA4(Eyes Absent 4:EYA transcription co-activated phosphatase 4 gene in bladder cancer cell line and transitional cell carcinoma (TCC) and its significance in clinicopathological features. Methods: methylation-specific polymerase chain reaction (EYA4) was used to detect the methylation status of EYA4 gene in 5 bladder cancer cell lines, 1 bladder immortalized epithelial cell line and 75 bladder transitional cell carcinoma tissues and adjacent tissues. Combined with clinicopathological features, statistical analysis was made. Results methylation was found in 4 of 5 bladder cancer cell lines. Its methylation rate was 80%; in human normal bladder cell line, methylation negative. EYA4 methylation rate was 70.7% 53% 75% in bladder cancer tissue, and 24% 75% in adjacent tissue, the difference was quite significant. The methylation of CpG island in the promoter region of P0.01. EYA4 gene was statistically significant in the clinicopathological features, such as single bladder tumor compared with multiple bladder tumor, bladder cancer tumor with diameter 鈮，

本文編號：1847379