本文選題：硬化蛋白 + 乳腺癌�。� 參考：《南方醫(yī)科大學(xué)學(xué)報(bào)》2017年08期

【摘要】：目的探討在體外乳腺癌骨轉(zhuǎn)移模型中抑制硬化蛋白基因的表達(dá)后對(duì)成骨細(xì)胞分化成熟的影響。方法以干擾硬化蛋白基因表達(dá)的si RNA腺病毒感染乳腺癌細(xì)胞株MDA-MB-231,RT-PCR檢測確認(rèn)硬化蛋白基因表達(dá)的改變。分別以正常培養(yǎng)的MDA-MB-231上清液,感染空病毒的MDA-MB-231上清液,感染SiSOST病毒的MDA-MB-231上清液與MG63或C3H10共培養(yǎng),并以成骨誘導(dǎo)培養(yǎng)基培養(yǎng)MG63及C3H10為陽性對(duì)照,普通培養(yǎng)基培養(yǎng)MG63及C3H10為陰性對(duì)照。定量PCR檢測成骨細(xì)胞分化相關(guān)指標(biāo)的改變,染色檢測堿性磷酸酶(ALP)表達(dá)的改變。結(jié)果感染Ad-si SOST病毒的MDA-MB-231細(xì)胞中硬化蛋白表達(dá)明顯降低。定量PCR結(jié)果顯示:與單獨(dú)培養(yǎng)的MG63相比,加成骨培養(yǎng)基后其骨鈣素、骨橋蛋白、護(hù)骨因子和整合素結(jié)合涎蛋白的表達(dá)均上升(P0.01)。在此基礎(chǔ)上,與231細(xì)胞上清液共培養(yǎng)后其表達(dá)降低(P0.01),差異具有統(tǒng)計(jì)學(xué)意義;MG63+231-RFP組結(jié)果一致,感染SiSOST病毒后,分化指標(biāo)重新上升(P0.01),差異具有統(tǒng)計(jì)學(xué)意義。C3H10細(xì)胞實(shí)驗(yàn)結(jié)果趨勢一致。MG63細(xì)胞與陰性對(duì)照組相比,分化誘導(dǎo)培養(yǎng)基可促進(jìn)ALP的分泌(P0.01),但與231細(xì)胞共培養(yǎng)后,ALP分泌減少(P0.01),感染Si-SOST后,可部分扭轉(zhuǎn)ALP分泌的減少(P0.01),ALP染色得到同樣的結(jié)果。C3H10細(xì)胞結(jié)果與MG63細(xì)胞趨勢一致。結(jié)論在體外乳腺癌骨轉(zhuǎn)移模型中,成骨細(xì)胞分化受阻;干擾硬化蛋白基因的表達(dá)有利于逆轉(zhuǎn)成骨細(xì)胞的分化受阻。
[Abstract]:Objective to investigate the effect of inhibition of sclerosin gene expression on differentiation and maturation of osteoblasts in bone metastases of breast cancer in vitro. Methods the expression of sclerosin gene was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) in breast cancer cell line MDA-MB-231infected with si RNA adenovirus which interfered with sclerosing protein gene expression. MDA-MB-231 supernatant of normal culture, MDA-MB-231 supernatant infected with empty virus, MDA-MB-231 supernatant infected with SiSOST virus co-cultured with MG63 or C3H10, and MG63 and C3H10 were cultured in osteogenic medium as positive control. MG63 and C3H10 in normal culture medium were negative control. Quantitative PCR was used to detect the changes of osteoblast differentiation and alkaline phosphatase (ALP) expression was detected by staining. Results the expression of sclerosin in MDA-MB-231 cells infected with Ad-si SOST virus was significantly decreased. The results of quantitative PCR showed that the expression of osteocalcin, osteopontin, osteopontin, osteoprotegerin and integrin-binding sialoprotein increased after addition of bone medium compared with MG63 cultured alone. On this basis, after co-culture with the supernatant of 231 cells, the expression of P0.01was decreased, and the difference was consistent with that of MG63 231-RFP group. After infection of MG63 231-RFP, the expression of MG63 231-RFP was similar to that of MG63 virus. Compared with the negative control group, the differentiation induction medium could promote the secretion of ALP, but after co-culture with 231 cells, the secretion of P0.01C was decreased, and after infection with Si-SOST, the differentiation and induction medium could decrease the secretion of P0.01C _ 3H _ (10) cells, and the results showed that the differentiation of MG63 cells was similar to that of the negative control group (P _ (0.01), but the differentiation induction medium could promote the secretion of ALP. The same result could be obtained by partially reversing the decrease of ALP secretion. The result of C3H10 cells was consistent with that of MG63 cells. Conclusion in the model of bone metastasis of breast cancer in vitro, the differentiation of osteoblasts is blocked, and the expression of interfering sclerosing protein gene is beneficial to reverse the obstruction of osteoblast differentiation.
【作者單位】： 重慶醫(yī)科大學(xué)病理生理學(xué)教研室;重慶醫(yī)科大學(xué)分子醫(yī)學(xué)與腫瘤研究中心;重慶醫(yī)科大學(xué)附屬第二醫(yī)院三腺外科;
【基金】：重慶市科技計(jì)劃項(xiàng)目(cstc2013jcyj A1009)
【分類號(hào)】：R737.9

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉燕,權(quán)瑞;乳腺癌骨轉(zhuǎn)移的放射治療35例[J];中國腫瘤臨床與康復(fù);2000年03期

2 陳輝利,趙青梅,杜光祖;乳腺癌骨轉(zhuǎn)移3年以上存活病例分析[J];華南國防醫(yī)學(xué)雜志;2000年01期

3 巨愛平;乳腺癌骨轉(zhuǎn)移12例綜合治療臨床觀察[J];臨床腫瘤學(xué)雜志;2001年01期

4 蔣國勤;乳腺癌骨轉(zhuǎn)移模型的建立[J];中國普外基礎(chǔ)與臨床雜志;2001年05期

5 王躍珍,孫曉江,劉林林,孫寶勝,呂鵬;208例乳腺癌骨轉(zhuǎn)移相關(guān)因素分析[J];中國實(shí)驗(yàn)診斷學(xué);2004年04期

6 柴楓;;乳腺癌骨轉(zhuǎn)移86例回顧性分析[J];實(shí)用醫(yī)藥雜志;2006年05期

7 徐海濱;胡祖健;胡望華;史紅仙;何俊玲;黃建;;乳腺癌骨轉(zhuǎn)移綜合治療初探[J];中華乳腺病雜志(電子版);2007年01期

8 葉謙;;100例乳腺癌患者核素骨顯像結(jié)果分析[J];實(shí)用醫(yī)技雜志;2007年09期

9 周圍;周恩相;王澤強(qiáng);郭瓊;;乳腺癌骨轉(zhuǎn)移57例回顧性分析[J];實(shí)用醫(yī)藥雜志;2007年05期

10 鄔健明;張勇;王偉;;乳腺癌骨轉(zhuǎn)移致股骨頸病理性骨折1例報(bào)告[J];西南軍醫(yī);2008年03期

相關(guān)會(huì)議論文 前10條

1 魏志真;董峰;;乳腺癌骨轉(zhuǎn)移的臨床特點(diǎn)分析[A];中華醫(yī)學(xué)會(huì)放射醫(yī)學(xué)與防護(hù)學(xué)分會(huì)第三次全中國青年學(xué)術(shù)交流會(huì)論文摘要匯編[C];2001年

2 魏志真;董峰;;乳腺癌骨轉(zhuǎn)移的臨床特點(diǎn)分析[A];中華醫(yī)學(xué)會(huì)放射醫(yī)學(xué)與防護(hù)學(xué)分會(huì)第三次全國中青年學(xué)術(shù)交流會(huì)論文匯編[C];2001年

3 徐海濱;胡祖健;胡望華;史紅仙;何俊玲;黃建;;乳腺癌骨轉(zhuǎn)移綜合治療初探[A];2005年浙江省外科學(xué)術(shù)會(huì)議論文匯編[C];2005年

4 蔡鋼;陳佳藝;朱驥;俞曉立;章真;郭小毛;胡超蘇;;乳腺癌骨轉(zhuǎn)移的放射治療和預(yù)后因素[A];2007第六屆全國放射腫瘤學(xué)學(xué)術(shù)年會(huì)論文集[C];2007年

5 蔡鋼;陳佳藝;朱驥;俞曉立;章真;郭小毛;胡超蘇;;乳腺癌骨轉(zhuǎn)移的放射治療和預(yù)后因素[A];2007第六屆全國放射腫瘤學(xué)學(xué)術(shù)年會(huì)論文集[C];2007年

6 劉兆U，

本文編號(hào)：1842699