本文選題：TRIP13 + HCT116�。� 參考：《吉林大學(xué)》2017年碩士論文

【摘要】：背景:從流行病學(xué)上來(lái)看,結(jié)直腸癌(colorectal cancer,CRC)的發(fā)病率和死亡率均令人堪憂。在我國(guó)所有惡性腫瘤之中,CRC的發(fā)病率位居第3-5位。在歐美國(guó)家所有惡性腫瘤中,CRC的發(fā)病率居第2-3位。在全球癌癥相關(guān)的死亡原因中,結(jié)直腸癌是第四大原因。多數(shù)CRC患者就診時(shí)已屬中晚期,經(jīng)手術(shù)治療、放療、化療等治療后發(fā)生腫瘤耐藥、進(jìn)展、復(fù)發(fā)幾率較高�；蛟\斷與治療為打破CRC傳統(tǒng)防治模式提供了一個(gè)新的方向,發(fā)現(xiàn)與CRC相關(guān)的基因并探究其致病機(jī)制是邁向這個(gè)方向的前提。甲狀腺激素受體因子13(Thyroid Hormone Receptor Interactor 13,TRIP13)基因位于5號(hào)染色體短臂1區(qū)5帶,它是一個(gè)蛋白編碼基因。TRIP13基因參與細(xì)胞減數(shù)分裂和有絲分裂的相關(guān)過(guò)程,能通過(guò)干擾紡錘體組裝檢查點(diǎn)進(jìn)而影響有絲分裂過(guò)程,從而導(dǎo)致腫瘤的發(fā)生、發(fā)展,它被確定為癌基因。TRIP13的過(guò)度表達(dá)可導(dǎo)致多種人類(lèi)癌癥,目前尚未證實(shí)TRIP13基因與CRC相關(guān),TRIP13基因與CRC的關(guān)系尚待進(jìn)一步研究。目的:本實(shí)驗(yàn)擬研究TRIP13基因在結(jié)直腸癌HCT116細(xì)胞中的表達(dá)情況,然后用慢病毒感染HCT116細(xì)胞的方法構(gòu)建TRIP13基因過(guò)表達(dá)和TRIP13基因敲減的HCT116細(xì)胞,并檢驗(yàn)慢病毒干擾效果。隨后,通過(guò)進(jìn)行一系列細(xì)胞功能檢測(cè)(包括細(xì)胞周期檢測(cè)、細(xì)胞凋亡檢測(cè)、克隆形成檢測(cè)、MTT檢測(cè))來(lái)研究TRIP13對(duì)胃癌細(xì)胞生物學(xué)特性的影響,探究干擾目的基因TRIP13對(duì)HCT116細(xì)胞增殖的影響,進(jìn)一步探討TRIP13對(duì)結(jié)直腸癌細(xì)胞的生物學(xué)作用,為后續(xù)結(jié)直腸癌基因診斷和治療提供初步實(shí)驗(yàn)依據(jù)。方法:(1)采用實(shí)時(shí)熒光定量檢測(cè)系統(tǒng)(Real-time Quantitative Polymerase Chain Reaction Detecting System,qPCR)檢測(cè)HCT116細(xì)胞中TRIP13基因的表達(dá),使用TRIP13基因RNA干擾序列的慢病毒感染目的細(xì)胞,再用qPCR檢測(cè)mRNA水平TRIP13基因敲減的效率,若TRIP13基因敲減效率50%,繼續(xù)進(jìn)行慢病毒感染;若TRIP13基因敲減效率50%,進(jìn)行下一步細(xì)胞功能檢測(cè)。(2)在體外細(xì)胞功能實(shí)驗(yàn)部分,細(xì)胞周期檢測(cè)使用碘化丙啶(Propidium,PI)染色流式細(xì)胞儀檢測(cè)法,來(lái)探究TRIP13基因?qū)CT116細(xì)胞生長(zhǎng)周期的影響;細(xì)胞凋亡檢測(cè)采用Annexin V-APC單染法,研究TRIP13基因與HCT116細(xì)胞凋亡的關(guān)系;克隆形成檢測(cè)使用Giemsa染色法,探究用含有目的基因RNA干擾序列的慢病毒感染后HCT116細(xì)胞的成瘤能力;通過(guò)MTT檢測(cè)探究TRIP13基因?qū)CT116細(xì)胞增殖的影響。(3)使用Spss 21.0軟件進(jìn)行統(tǒng)計(jì)分析。結(jié)果:(1)HCT116細(xì)胞中高豐度表達(dá)TRIP13基因,慢病毒感染HCT116細(xì)胞效率達(dá)到80%以上,細(xì)胞狀態(tài)正常,HCT116細(xì)胞中TRIP13基因在mRNA水平的表達(dá)量下降(p0.05),敲減效率達(dá)到85.5%。(2)細(xì)胞周期檢測(cè)結(jié)果:相比對(duì)照組(shCtrl組),實(shí)驗(yàn)組(shTRIP13組)處于S期的細(xì)胞減少(P0.05),處于G1期的細(xì)胞增加(P0.05),處于G2/M期的細(xì)胞無(wú)顯著變化。(3)細(xì)胞凋亡檢測(cè)結(jié)果:與shCtrl組相比,shTRIP13組凋亡細(xì)胞數(shù)增多(P0.05)。(4)克隆形成檢測(cè)結(jié)果:與shCtrl組相比,shTRIP13組克隆形成數(shù)減少(P0.05)。(5)MTT檢測(cè)結(jié)果:相比shCtrl組,shTRIP13組細(xì)胞增殖減緩。結(jié)論:干擾目的基因TRIP13抑制結(jié)直腸癌細(xì)胞HCT116的增殖。TRIP13基因?yàn)榻Y(jié)直腸癌的的防治提供了一個(gè)新方向,它可能是CRC治療的一個(gè)潛在靶點(diǎn),為實(shí)現(xiàn)CRC的可靠診療具有基礎(chǔ)的實(shí)驗(yàn)依據(jù)。
[Abstract]:Background: the incidence and mortality of colorectal cancer (CRC) are all worrying in epidemiology. Among all the malignant tumors of our country, the incidence of CRC is the No. 3-5. Among all the malignant tumors in Europe and America, the incidence of CRC is the 2-3. The colorectal cancer is fourth among the causes of global cancer related deaths. Most CRC patients are in the middle and late stages of medical treatment. After surgical treatment, radiotherapy, chemotherapy and other treatments, tumor resistance, progression, and recurrence rate are higher. Gene diagnosis and treatment provide a new direction for breaking the traditional CRC prevention and control model, and finding the genes related to CRC and exploring its pathogenesis are the premise of this direction. The thyroid hormone receptor factor 13 (Thyroid Hormone Receptor Interactor 13, TRIP13) gene is located in the 5 band of the short arm 1 of chromosome 5. It is a protein encoding gene.TRIP13 gene involved in cell meiosis and mitosis. It can interfere with the spindle assembly checkpoint and then affect the mitosis process, resulting in swelling. The occurrence and development of the tumor, which is identified as the overexpression of the oncogene.TRIP13, can lead to a variety of human cancers. At present, the TRIP13 gene has not been confirmed to be associated with CRC. The relationship between the TRIP13 gene and CRC remains to be further studied. Objective: To study the expression of the TRIP13 gene in HCT116 cells in colorectal cancer and to infect HCT116 with lentivirus. The cell method constructs the TRIP13 gene overexpression and the TRIP13 gene knockout HCT116 cells, and tests the effect of the lentivirus interference. Subsequently, a series of cell function tests (including cell cycle detection, cell apoptosis detection, clone formation detection, MTT detection) are used to study the effects of TRIP13 on the biological characteristics of gastric cancer cells and explore the interference. The effect of target gene TRIP13 on the proliferation of HCT116 cells, further explore the biological effect of TRIP13 on colorectal cancer cells, and provide a preliminary experimental basis for the follow-up of colorectal cancer gene diagnosis and treatment. Methods: (1) real-time quantitative detection system (Real-time Quantitative Polymerase Chain Reaction Detecting System, qPCR) detection The expression of TRIP13 gene in HCT116 cells was detected by using the TRIP13 gene RNA interference sequence to infect the target cells, and then qPCR was used to detect the efficiency of the mRNA level TRIP13 gene knockout. If TRIP13 gene knockout efficiency was 50%, the slow virus infection continued. If the TRIP13 gene knockout efficiency was 50%, the next step cell function detection. (2) in vitro fines. In cell function experiment part, cell cycle detection using Propidium (PI) staining flow cytometer detection method to explore the effect of TRIP13 gene on the growth cycle of HCT116 cells; cell apoptosis detection using Annexin V-APC single staining method to study the relationship between TRIP13 gene and HCT116 cell apoptosis; clone formation detection using Giemsa staining method, To explore the tumorigenicity of HCT116 cells after lentivirus infection with the target gene RNA interference sequence; explore the effect of TRIP13 gene on the proliferation of HCT116 cells by MTT detection. (3) the use of Spss 21 software for statistical analysis. Results: (1) the high abundance of TRIP13 gene in HCT116 cells, and the efficiency of the lentivirus infection HCT116 cells more than 80% The expression of TRIP13 gene in HCT116 cells decreased (P0.05) in HCT116 cells, and the knockout efficiency reached 85.5%. (2) cell cycle detection results: compared with the control group (shCtrl group), the cells in the experimental group (group shTRIP13) were in S phase (P0.05), the cells in the G1 phase increased (P0.05), and there was no significant change in the cells in the G2/M stage. (3) thin. Apoptosis detection results: compared with the shCtrl group, the number of apoptotic cells in the shTRIP13 group increased (P0.05). (4) the clone formation detection results: compared with the shCtrl group, the number of clones in the shTRIP13 group decreased (P0.05). (5) MTT detection results: compared with the shCtrl group, the cell proliferation slowed down in the shTRIP13 group. Conclusion: the interference target gene TRIP13 inhibits the HCT116 of colorectal cancer cells. The proliferation of.TRIP13 gene provides a new direction for the prevention and control of colorectal cancer. It may be a potential target for the treatment of CRC and is the basis for the reliable diagnosis and treatment of CRC.

【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R735.34

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本文編號(hào)：1839467