本文選題：結(jié)腸腫瘤 + 分化抑制蛋白　； 參考：《中國(guó)全科醫(yī)學(xué)》2017年08期

【摘要】：背景既往研究已經(jīng)證實(shí)DNA結(jié)合分化抑制蛋白1(Id-1)表達(dá)升高在結(jié)直腸癌的發(fā)生、發(fā)展中發(fā)揮重要作用,但I(xiàn)d-1對(duì)結(jié)腸癌細(xì)胞侵襲行為影響及機(jī)制的研究較少。目的探討下調(diào)Id-1基因?qū)θ私Y(jié)腸癌SW480細(xì)胞株增殖、轉(zhuǎn)移、侵襲能力的影響及作用機(jī)制。方法 2016年1—6月,人結(jié)腸癌SW480細(xì)胞株分為3組:空白組,未進(jìn)行轉(zhuǎn)染;空載體組,用空載體進(jìn)行轉(zhuǎn)染;抑制組,采用Id-1特異小干擾RNA(siRNA)進(jìn)行轉(zhuǎn)染,采用反轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)和Western blotting法檢測(cè)各組細(xì)胞Id-1和Ki-67基因及蛋白的表達(dá)情況,采用MTT法檢測(cè)細(xì)胞增殖能力,采用細(xì)胞劃痕實(shí)驗(yàn)、Transwell小室和Matrigel侵襲實(shí)驗(yàn)評(píng)價(jià)Id-1對(duì)細(xì)胞轉(zhuǎn)移和侵襲能力的影響。結(jié)果 3組細(xì)胞Id-1、Ki-67 mRNA及其相應(yīng)蛋白表達(dá)水平比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);抑制組細(xì)胞Id-1、Ki-67 mRNA及其相應(yīng)蛋白表達(dá)水平較空白組、空載體組降低(P0.05)。培養(yǎng)第1、2天,3組細(xì)胞增殖吸光度(OD值)比較,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。培養(yǎng)第3~7天,3組細(xì)胞增殖OD值比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);其中抑制組細(xì)胞增殖OD值較空白組及空載體組降低(P0.05)。細(xì)胞劃痕實(shí)驗(yàn)結(jié)果顯示,抑制組細(xì)胞緩慢向中央遷移,缺損處修復(fù)較緩慢,空白組和空載體組細(xì)胞向劃痕處的遷移速度明顯加快,而空白組與空載體組間的遷移速度差異不明顯。Transwell小室和Matrigel侵襲實(shí)驗(yàn)表明,3組遷移細(xì)胞數(shù)量和侵襲細(xì)胞數(shù)量比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);抑制組遷移細(xì)胞數(shù)量和侵襲細(xì)胞數(shù)量較空白組、空載體組減少(P0.05)。結(jié)論下調(diào)Id-1基因能抑制人結(jié)腸癌SW480細(xì)胞株的增殖、轉(zhuǎn)移和侵襲能力,其機(jī)制可能與抑制Ki-67的表達(dá)有關(guān)。
[Abstract]:Background research has confirmed that the increase of DNA binding inhibitor protein 1 (Id-1) expression plays an important role in the development of colorectal cancer. However, there are few studies on the impact of Id-1 on the invasion of colon cancer cells and its mechanism. The purpose of this study is to investigate the effect of down regulation of Id-1 gene on the proliferation, metastasis and invasion of human colon cancer cell line. Methods from 1 to June 2016, human colon cancer SW480 cell lines were divided into 3 groups: blank group, untransfected, empty carrier group, transfected with empty carrier, inhibition group, Id-1 specific small interference RNA (siRNA) transfection, reverse transcription polymerase chain reaction (RT-PCR) and Western blotting method to detect Id-1 and Ki-67 genes and eggs of each group. In white expression, MTT assay was used to detect cell proliferation, cell scratch test, Transwell chamber and Matrigel invasion test to evaluate the effect of Id-1 on cell metastasis and invasion. Results 3 groups of cells, Id-1, Ki-67 mRNA and corresponding protein expression levels were compared, the difference was statistically significant (P0.05), Id-1, Ki-67 in the inhibition group, Ki-67. The expression level of mRNA and its corresponding protein was lower than that in the blank group (P0.05). There was no significant difference between the 3 groups of cell proliferation absorbency (P0.05). On day 3~7, the proliferation of cells in the 3 groups was statistically significant (P0.05), and the proliferation of cell proliferation in the inhibition group was more than that in the blank group and the empty body. The group decreased (P0.05). The results of cell scratch test showed that the cells migrated slowly to the center and the repair of the defect was slow. The migration speed of the cells in the blank group and the empty carrier group was quicker than that of the blank group and the empty carrier group. The difference of the migration velocity between the blank group and the empty carrier group was not obvious in the.Transwell chamber and the Matrigel invasion experiment, which showed that the 3 groups migrated fine. The number of cells and the number of invasive cells were statistically significant (P0.05); the number of cells and the number of invasive cells in the inhibition group were less than that in the blank group, and the number of the unloaded body decreased (P0.05). Conclusion the down-regulation of Id-1 gene could inhibit the proliferation, metastasis and invasion of human colon cancer SW480 cell lines, and the mechanism may be related to the inhibition of the expression of Ki-67.

【作者單位】： 河北北方學(xué)院附屬第一醫(yī)院血管腺體外科;河北北方學(xué)院附屬第一醫(yī)院超聲科;河北北方學(xué)院附屬第一醫(yī)院胃腸外科;河北北方學(xué)院附屬第一醫(yī)院病理科;
【基金】：河北省科技支撐計(jì)劃資助項(xiàng)目(152777237) 河北省衛(wèi)計(jì)委醫(yī)學(xué)科學(xué)研究重點(diǎn)課題計(jì)劃(20150058) 河北省張家口市科技局指令性計(jì)劃(1311055D)
【分類號(hào)】：R735.35

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