本文選題：結腸腫瘤 + 分化抑制蛋白��； 參考：《中國全科醫(yī)學》2017年08期

【摘要】：背景既往研究已經證實DNA結合分化抑制蛋白1(Id-1)表達升高在結直腸癌的發(fā)生、發(fā)展中發(fā)揮重要作用,但Id-1對結腸癌細胞侵襲行為影響及機制的研究較少。目的探討下調Id-1基因對人結腸癌SW480細胞株增殖、轉移、侵襲能力的影響及作用機制。方法 2016年1—6月,人結腸癌SW480細胞株分為3組:空白組,未進行轉染;空載體組,用空載體進行轉染;抑制組,采用Id-1特異小干擾RNA(siRNA)進行轉染,采用反轉錄聚合酶鏈式反應(RT-PCR)和Western blotting法檢測各組細胞Id-1和Ki-67基因及蛋白的表達情況,采用MTT法檢測細胞增殖能力,采用細胞劃痕實驗、Transwell小室和Matrigel侵襲實驗評價Id-1對細胞轉移和侵襲能力的影響。結果 3組細胞Id-1、Ki-67 mRNA及其相應蛋白表達水平比較,差異均有統(tǒng)計學意義(P0.05);抑制組細胞Id-1、Ki-67 mRNA及其相應蛋白表達水平較空白組、空載體組降低(P0.05)。培養(yǎng)第1、2天,3組細胞增殖吸光度(OD值)比較,差異均無統(tǒng)計學意義(P0.05)。培養(yǎng)第3~7天,3組細胞增殖OD值比較,差異均有統(tǒng)計學意義(P0.05);其中抑制組細胞增殖OD值較空白組及空載體組降低(P0.05)。細胞劃痕實驗結果顯示,抑制組細胞緩慢向中央遷移,缺損處修復較緩慢,空白組和空載體組細胞向劃痕處的遷移速度明顯加快,而空白組與空載體組間的遷移速度差異不明顯。Transwell小室和Matrigel侵襲實驗表明,3組遷移細胞數量和侵襲細胞數量比較,差異均有統(tǒng)計學意義(P0.05);抑制組遷移細胞數量和侵襲細胞數量較空白組、空載體組減少(P0.05)。結論下調Id-1基因能抑制人結腸癌SW480細胞株的增殖、轉移和侵襲能力,其機制可能與抑制Ki-67的表達有關。
[Abstract]:Background research has confirmed that the increase of DNA binding inhibitor protein 1 (Id-1) expression plays an important role in the development of colorectal cancer. However, there are few studies on the impact of Id-1 on the invasion of colon cancer cells and its mechanism. The purpose of this study is to investigate the effect of down regulation of Id-1 gene on the proliferation, metastasis and invasion of human colon cancer cell line. Methods from 1 to June 2016, human colon cancer SW480 cell lines were divided into 3 groups: blank group, untransfected, empty carrier group, transfected with empty carrier, inhibition group, Id-1 specific small interference RNA (siRNA) transfection, reverse transcription polymerase chain reaction (RT-PCR) and Western blotting method to detect Id-1 and Ki-67 genes and eggs of each group. In white expression, MTT assay was used to detect cell proliferation, cell scratch test, Transwell chamber and Matrigel invasion test to evaluate the effect of Id-1 on cell metastasis and invasion. Results 3 groups of cells, Id-1, Ki-67 mRNA and corresponding protein expression levels were compared, the difference was statistically significant (P0.05), Id-1, Ki-67 in the inhibition group, Ki-67. The expression level of mRNA and its corresponding protein was lower than that in the blank group (P0.05). There was no significant difference between the 3 groups of cell proliferation absorbency (P0.05). On day 3~7, the proliferation of cells in the 3 groups was statistically significant (P0.05), and the proliferation of cell proliferation in the inhibition group was more than that in the blank group and the empty body. The group decreased (P0.05). The results of cell scratch test showed that the cells migrated slowly to the center and the repair of the defect was slow. The migration speed of the cells in the blank group and the empty carrier group was quicker than that of the blank group and the empty carrier group. The difference of the migration velocity between the blank group and the empty carrier group was not obvious in the.Transwell chamber and the Matrigel invasion experiment, which showed that the 3 groups migrated fine. The number of cells and the number of invasive cells were statistically significant (P0.05); the number of cells and the number of invasive cells in the inhibition group were less than that in the blank group, and the number of the unloaded body decreased (P0.05). Conclusion the down-regulation of Id-1 gene could inhibit the proliferation, metastasis and invasion of human colon cancer SW480 cell lines, and the mechanism may be related to the inhibition of the expression of Ki-67.

【作者單位】： 河北北方學院附屬第一醫(yī)院血管腺體外科;河北北方學院附屬第一醫(yī)院超聲科;河北北方學院附屬第一醫(yī)院胃腸外科;河北北方學院附屬第一醫(yī)院病理科;
【基金】：河北省科技支撐計劃資助項目(152777237) 河北省衛(wèi)計委醫(yī)學科學研究重點課題計劃(20150058) 河北省張家口市科技局指令性計劃(1311055D)
【分類號】：R735.35

【參考文獻】

相關期刊論文 前10條

1 武雪亮;王立坤;薛軍;楊東東;屈明;郭飛;楊瑞敏;劉博;;DNA結合分化抑制蛋白1和血管內皮生長因子的表達及其與腫瘤微血管生成的相關性研究[J];中國臨床藥理學雜志;2016年05期

2 武雪亮;王立坤;薛軍;楊東東;屈明;郭飛;楊瑞敏;劉博;候占富;;Id-1在結直腸腺癌、腺瘤、正常黏膜中的表達及意義[J];安徽醫(yī)科大學學報;2016年02期

3 薛軍;武雪亮;高曉斌;王立坤;屈明;郭飛;張鵬程;楊瑞敏;袁美錦;;結直腸腺癌組織survivin和Ki-67的表達及其與預后的關系[J];基礎醫(yī)學與臨床;2015年11期

4 張s，

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