本文選題：低劑量輻射 + 適應性效應�。� 參考：《山西醫(yī)科大學》2017年碩士論文

【摘要】：目的:低劑量輻射對正常細胞的適應性效應及興奮效應是當前研究的熱點,由于其適應性效應可顯著降低放療對正常細胞的損傷,同時對腫瘤細胞的有一定抑制作用,導致其適應性效應在臨床研究中成為熱點。然而低劑量輻射生物效應還包括超敏感性、旁效應等,影響其生物學效應的因素包括輻射劑量、輻射品質以及生物本身的特異性。一方面我們看到了低劑量輻射在臨床及社會實際生活的廣闊應用前景,一方面低劑量輻射的效應機制仍有諸多未明之處,導致難以對其進行令人信服的損傷評價,極大限制了它的應用前景。因此對低劑量輻射的研究仍需深入。本研究觀察體外實驗低劑量輻射對Lewis細胞生長速度、成活率、遷移及侵襲能力的影響,同時通過體內試驗觀察低劑量輻射與惡性腫瘤雙重影響下免疫機體機能的變化情況。方法:本實驗分為體外和體內試驗兩部分,體外實驗將Lewis細胞分為四組,分別以0 Gy、12.5 mGy、25 mGy、50 mGy劑量伽馬射線按兩天照射一次的頻率照射15次后,以克隆形成率、MTT實驗、劃痕實驗、transwell實驗檢驗細胞成活率、生長速率、遷移能力、侵襲力變化情況。體內試驗分兩批進行,一批將經照射后細胞植入小鼠腋下,另一批將未經處理的腫瘤細胞植入經照射小鼠腋下。然后觀察記錄小鼠及腫瘤各項指標。結果:1.體外實驗克隆形成率實驗組按劑量不同分別較對照組降低17.8%、21.8%、65.0%,生長速率較對照組降低9.4%、22.5%、25.7%,遷移能力較對照組提高65.8%、90.9%、125.2%。,侵襲能力較對照組增強90.2%、116.6%、193.8%。體內試驗切片可見部分接種照射細胞的小鼠有肺部轉移,對照組則無。2.溶酶體染色實驗48h測得吸光值與0 Gy相比12.5 mGy、25 mGy和50mGy分別增加8.6%、14.6%、17.8%。72h測得吸光值與0 Gy相比12.5 mGy、25 mGy和50mGy分別增加14.3%、17.0%、24.4%。3.ELSA實驗實驗組小鼠與對照組有顯著不同,其中IL-6在12.5 mGy、25 mGy和50mGy組中較0Gy組分別增加55.6%、114.8%、229.6%。IFN-γ在12.5 mGy、25 mGy和50mGy組中較0Gy組分別增加32.2%、98.9%、133.3%。測得各組腫瘤重量12.5 mGy、25 mGy和50mGy較0Gy組分別減少15.9%、40.6%、63.4%。結論:體外實驗說明低劑量輻射對腫瘤細胞的增值能力有明顯抑制,但對腫瘤細胞的遷移侵襲能力有增強作用,體內試驗也證明了這點。體內試驗通過組間小鼠對比,低劑量輻射對小鼠免疫力有增強作用。
[Abstract]:Objective: the adaptive effects and excitatory effects of low dose radiation on normal cells are the focus of current research. Because of their adaptive effects, the damage to normal cells caused by radiotherapy can be significantly reduced, and the tumor cells can be inhibited to a certain extent. As a result, its adaptive effect has become a hot spot in clinical research. However, biological effects of low dose radiation also include hypersensitivity and side effects. The biological effects of low dose radiation include radiation dose, radiation quality and biological specificity. On the one hand, we see the broad application prospect of low dose radiation in clinical and social life, on the other hand, the mechanism of low dose radiation is still not clear, which makes it difficult to make convincing damage evaluation. The prospect of its application is greatly limited. Therefore, the study of low dose radiation still needs to be further studied. In this study, we observed the effects of low dose radiation on the growth rate, survival rate, migration and invasion ability of Lewis cells in vitro, and observed the changes of immune function under the double effects of low dose radiation and malignant tumor in vivo. Methods: in vitro and in vivo experiments, Lewis cells were divided into four groups. Lewis cells were irradiated at a dose of 0 Gy 12.5 mGy ~ (25 mGy) ~ (50 mGy) for 15 times at a frequency of one day for 15 times, and the colony formation rate was determined by MTT assay. The cell survival rate, growth rate, migration ability and invasiveness were examined by scratch test and transwell test. In vivo experiments were carried out in two batches. One batch of irradiated cells were implanted into the armpit of mice and the other batch of untreated tumor cells were implanted into the axillary of irradiated mice. Then the indexes of mice and tumor were observed and recorded. The result is 1: 1. Compared with the control group, the rate of colony formation in vitro in the experimental group was lower than that in the control group by 17.81.821.80.The growth rate of the experimental group was lower than that of the control group, the growth rate was lower than that of the control group, the migration capacity of the experimental group was increased by 65.80.90. 9% and 125.2%, and the invasiveness of the experimental group was increased by 90.26.6193.83.85.7than that of the control group. In vivo test sections showed that some of the mice inoculated with irradiated cells had lung metastasis, while the control group had no. 2. 2. After 48 hours of lysosomal staining, the absorptivity values of 12.5 mGy (25 mGy) and 12.5mGy (25 mGy) and 50mGy (50mGy) of lysosome staining were increased by 8.6and 14.6and 14.6and 17.8g / 72h, respectively. Compared with 0 Gy, the absorbency values measured at 12.5mGy 25 mGy and 50mGy increased 14.3Gy 17.0g 24.44.3.There were significant differences between the experimental group and the control group. IL-6 in 12.5 mGy 25 mGy and 50mGy group increased 55.6and 114.8% and 229.6%, respectively. IFN- 緯 in 12.5 mGy 25 mGy group and 50mGy group increased 32.299% and 133.3%, respectively, compared with 0Gy group. The tumor weight of each group was 12.5mGy ~ 25 mGy and 50mGy decreased 15.9mGy ~ (25) and 63.40.The tumor weight of each group decreased by 15.9mGy ~ (25) mGy and 50mGy respectively. Conclusion: in vitro experiments showed that low dose radiation significantly inhibited the proliferation of tumor cells, but enhanced the ability of migration and invasion of tumor cells, which was also proved by in vivo experiments. In vivo test, low dose radiation enhanced immunity of mice by comparison between groups.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R730.5

【參考文獻】

相關期刊論文 前10條

1 許建;李衛(wèi)國;李鵬;馮憲勛;李潔清;;部分不同工種放射工作人員輻射效應分析[J];中國輻射衛(wèi)生;2016年05期

2 藍綠琴;蔣發(fā)燁;陳秀梅;劉睿;;肝癌患者植入I~(125)粒子后周圍環(huán)境輻射劑量率的檢測與防護研究[J];護理學報;2016年18期

3 王萍;袁德曉;邵春林;;染色質結構影響腫瘤細胞輻射敏感性的研究進展[J];中華放射醫(yī)學與防護雜志;2016年09期

4 張欣;趙永成;;低劑量電離輻射對放射工作人員外周血細胞影響的Meta分析[J];中國輻射衛(wèi)生;2016年04期

5 張海兵;辛勇;黃倩;郭雯雯;章龍珍;;低劑量X射線照射對K562細胞周期及凋亡影響的實驗研究[J];中華腫瘤防治雜志;2016年07期

6 李廣文;李卉;李剛;王軍;楊朝艷;何偉;田青;齊志遠;王東平;呂波濤;裴永泉;梁政;程碩;;低劑量慢性輻射環(huán)境工作人員口腔醫(yī)療需要自我評估情況問卷調查分析[J];臨床軍醫(yī)雜志;2016年02期

7 陳萬青;鄭榮壽;張思維;曾紅梅;左婷婷;賈漫漫;夏昌發(fā);鄒小農;赫捷;;2012年中國惡性腫瘤發(fā)病和死亡分析[J];中國腫瘤;2016年01期

8 潘婷婷;尹榮華;董小明;趙珂;詹軼群;楊曉明;李長燕;;紅細胞分化相關基因敲除小鼠對低劑量輻射損傷敏感性的研究[J];軍事醫(yī)學;2015年06期

9 薛丹;沈秀;徐文清;;輻射損傷防護藥物的研究進展[J];中華放射醫(yī)學與防護雜志;2012年06期

10 趙航;賀中民;涂宏海;王四旺;謝艷華;;小鼠低劑量輻射損傷模型的初步研究[J];現(xiàn)代生物醫(yī)學進展;2012年20期

，

本文編號：1831945