本文選題：低劑量輻射 + 適應(yīng)性效應(yīng)��； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:低劑量輻射對(duì)正常細(xì)胞的適應(yīng)性效應(yīng)及興奮效應(yīng)是當(dāng)前研究的熱點(diǎn),由于其適應(yīng)性效應(yīng)可顯著降低放療對(duì)正常細(xì)胞的損傷,同時(shí)對(duì)腫瘤細(xì)胞的有一定抑制作用,導(dǎo)致其適應(yīng)性效應(yīng)在臨床研究中成為熱點(diǎn)。然而低劑量輻射生物效應(yīng)還包括超敏感性、旁效應(yīng)等,影響其生物學(xué)效應(yīng)的因素包括輻射劑量、輻射品質(zhì)以及生物本身的特異性。一方面我們看到了低劑量輻射在臨床及社會(huì)實(shí)際生活的廣闊應(yīng)用前景,一方面低劑量輻射的效應(yīng)機(jī)制仍有諸多未明之處,導(dǎo)致難以對(duì)其進(jìn)行令人信服的損傷評(píng)價(jià),極大限制了它的應(yīng)用前景。因此對(duì)低劑量輻射的研究仍需深入。本研究觀察體外實(shí)驗(yàn)低劑量輻射對(duì)Lewis細(xì)胞生長(zhǎng)速度、成活率、遷移及侵襲能力的影響,同時(shí)通過(guò)體內(nèi)試驗(yàn)觀察低劑量輻射與惡性腫瘤雙重影響下免疫機(jī)體機(jī)能的變化情況。方法:本實(shí)驗(yàn)分為體外和體內(nèi)試驗(yàn)兩部分,體外實(shí)驗(yàn)將Lewis細(xì)胞分為四組,分別以0 Gy、12.5 mGy、25 mGy、50 mGy劑量伽馬射線按兩天照射一次的頻率照射15次后,以克隆形成率、MTT實(shí)驗(yàn)、劃痕實(shí)驗(yàn)、transwell實(shí)驗(yàn)檢驗(yàn)細(xì)胞成活率、生長(zhǎng)速率、遷移能力、侵襲力變化情況。體內(nèi)試驗(yàn)分兩批進(jìn)行,一批將經(jīng)照射后細(xì)胞植入小鼠腋下,另一批將未經(jīng)處理的腫瘤細(xì)胞植入經(jīng)照射小鼠腋下。然后觀察記錄小鼠及腫瘤各項(xiàng)指標(biāo)。結(jié)果:1.體外實(shí)驗(yàn)克隆形成率實(shí)驗(yàn)組按劑量不同分別較對(duì)照組降低17.8%、21.8%、65.0%,生長(zhǎng)速率較對(duì)照組降低9.4%、22.5%、25.7%,遷移能力較對(duì)照組提高65.8%、90.9%、125.2%。,侵襲能力較對(duì)照組增強(qiáng)90.2%、116.6%、193.8%。體內(nèi)試驗(yàn)切片可見部分接種照射細(xì)胞的小鼠有肺部轉(zhuǎn)移,對(duì)照組則無(wú)。2.溶酶體染色實(shí)驗(yàn)48h測(cè)得吸光值與0 Gy相比12.5 mGy、25 mGy和50mGy分別增加8.6%、14.6%、17.8%。72h測(cè)得吸光值與0 Gy相比12.5 mGy、25 mGy和50mGy分別增加14.3%、17.0%、24.4%。3.ELSA實(shí)驗(yàn)實(shí)驗(yàn)組小鼠與對(duì)照組有顯著不同,其中IL-6在12.5 mGy、25 mGy和50mGy組中較0Gy組分別增加55.6%、114.8%、229.6%。IFN-γ在12.5 mGy、25 mGy和50mGy組中較0Gy組分別增加32.2%、98.9%、133.3%。測(cè)得各組腫瘤重量12.5 mGy、25 mGy和50mGy較0Gy組分別減少15.9%、40.6%、63.4%。結(jié)論:體外實(shí)驗(yàn)說(shuō)明低劑量輻射對(duì)腫瘤細(xì)胞的增值能力有明顯抑制,但對(duì)腫瘤細(xì)胞的遷移侵襲能力有增強(qiáng)作用,體內(nèi)試驗(yàn)也證明了這點(diǎn)。體內(nèi)試驗(yàn)通過(guò)組間小鼠對(duì)比,低劑量輻射對(duì)小鼠免疫力有增強(qiáng)作用。
[Abstract]:Objective: the adaptive effects and excitatory effects of low dose radiation on normal cells are the focus of current research. Because of their adaptive effects, the damage to normal cells caused by radiotherapy can be significantly reduced, and the tumor cells can be inhibited to a certain extent. As a result, its adaptive effect has become a hot spot in clinical research. However, biological effects of low dose radiation also include hypersensitivity and side effects. The biological effects of low dose radiation include radiation dose, radiation quality and biological specificity. On the one hand, we see the broad application prospect of low dose radiation in clinical and social life, on the other hand, the mechanism of low dose radiation is still not clear, which makes it difficult to make convincing damage evaluation. The prospect of its application is greatly limited. Therefore, the study of low dose radiation still needs to be further studied. In this study, we observed the effects of low dose radiation on the growth rate, survival rate, migration and invasion ability of Lewis cells in vitro, and observed the changes of immune function under the double effects of low dose radiation and malignant tumor in vivo. Methods: in vitro and in vivo experiments, Lewis cells were divided into four groups. Lewis cells were irradiated at a dose of 0 Gy 12.5 mGy ~ (25 mGy) ~ (50 mGy) for 15 times at a frequency of one day for 15 times, and the colony formation rate was determined by MTT assay. The cell survival rate, growth rate, migration ability and invasiveness were examined by scratch test and transwell test. In vivo experiments were carried out in two batches. One batch of irradiated cells were implanted into the armpit of mice and the other batch of untreated tumor cells were implanted into the axillary of irradiated mice. Then the indexes of mice and tumor were observed and recorded. The result is 1: 1. Compared with the control group, the rate of colony formation in vitro in the experimental group was lower than that in the control group by 17.81.821.80.The growth rate of the experimental group was lower than that of the control group, the growth rate was lower than that of the control group, the migration capacity of the experimental group was increased by 65.80.90. 9% and 125.2%, and the invasiveness of the experimental group was increased by 90.26.6193.83.85.7than that of the control group. In vivo test sections showed that some of the mice inoculated with irradiated cells had lung metastasis, while the control group had no. 2. 2. After 48 hours of lysosomal staining, the absorptivity values of 12.5 mGy (25 mGy) and 12.5mGy (25 mGy) and 50mGy (50mGy) of lysosome staining were increased by 8.6and 14.6and 14.6and 17.8g / 72h, respectively. Compared with 0 Gy, the absorbency values measured at 12.5mGy 25 mGy and 50mGy increased 14.3Gy 17.0g 24.44.3.There were significant differences between the experimental group and the control group. IL-6 in 12.5 mGy 25 mGy and 50mGy group increased 55.6and 114.8% and 229.6%, respectively. IFN- 緯 in 12.5 mGy 25 mGy group and 50mGy group increased 32.299% and 133.3%, respectively, compared with 0Gy group. The tumor weight of each group was 12.5mGy ~ 25 mGy and 50mGy decreased 15.9mGy ~ (25) and 63.40.The tumor weight of each group decreased by 15.9mGy ~ (25) mGy and 50mGy respectively. Conclusion: in vitro experiments showed that low dose radiation significantly inhibited the proliferation of tumor cells, but enhanced the ability of migration and invasion of tumor cells, which was also proved by in vivo experiments. In vivo test, low dose radiation enhanced immunity of mice by comparison between groups.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R730.5

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