本文選題：胃腫瘤 + 人線粒體轉錄終止因子�。� 參考：《臨床與實驗病理學雜志》2017年01期

【摘要】：目的分析hMTERF3 mRNA和蛋白質在胃癌組織及正常組織中的表達,探討其與胃癌發(fā)生、發(fā)展的關系。方法分別采用半定量RT-PCR和免疫組化SP法,檢測61例胃癌組織及正常組織中hMTERF3 mRNA和蛋白質表達水平,分析hMTERF3表達與胃癌臨床分期、病理分級、肌層浸潤和淋巴結轉移的關系。結果 hMTERF3 mRNA和蛋白質在胃癌組織中的表達水平顯著高于正常胃組織。hMTERF3蛋白在正常胃組織陽性率為24.6%(15/61),胃癌組織中的陽性率為86.9%(53/61),差異具有統(tǒng)計學意義。hMTERF3蛋白的表達與胃癌臨床分期、病理分級、肌層浸潤和淋巴結轉移有關。結論 hMTERF3蛋白的表達異�？赡苁俏赴┌l(fā)生、發(fā)展的分子機制之一,可為預測胃癌的發(fā)生提供可能的生物學標志物,也可為胃癌基因診斷和基因治療提供潛在的靶標。
[Abstract]:Objective to analyze the expression of hMTERF3 mRNA and protein in gastric cancer tissues and normal tissues, and to explore the relationship between hMTERF3 mRNA and gastric carcinogenesis and development. Methods Semi-quantitative RT-PCR and immunohistochemical SP method were used to detect the expression of hMTERF3 mRNA and protein in 61 cases of gastric cancer and normal tissues. The relationship between the expression of hMTERF3 and clinical stage, pathological grade, myometrial infiltration and lymph node metastasis of gastric cancer was analyzed. Results the expression of hMTERF3 mRNA and protein in gastric cancer was significantly higher than that in normal gastric tissue (24.615 / 61) and in gastric cancer (86.9% / 53 / 61). The difference was statistically significant in the expression of hMTERF3 protein and the clinical stage of gastric cancer. Pathological grade, myometrial invasion and lymph node metastasis were related. Conclusion the abnormal expression of hMTERF3 protein may be one of the molecular mechanisms of gastric carcinogenesis and development, which may provide a possible biomarker for predicting the occurrence of gastric cancer, as well as a potential target for gene diagnosis and gene therapy of gastric cancer.
【作者單位】： 大理大學基礎醫(yī)學院生物化學與分子生物學教研室;云南省昆蟲生物醫(yī)藥研發(fā)重點實驗室;云南省大理市第一人民醫(yī)院呼吸科;
【基金】：國家自然科學基金(81560458、31601155) 云南省教育廳科學研究基金重點項目(2014Z126、2016ZDX101、2016ZDX105) 大理大學博士科研啟動基金(BSKY2012018);大理大學大學生創(chuàng)新創(chuàng)業(yè)計劃(CXCYX-2016-18)
【分類號】：R735.2
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本文編號：1826533