發(fā)布時(shí)間：2018-04-30 20:03

  本文選題：DNA甲基化 + 膠質(zhì)瘤��； 參考：《安徽醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的探討血漿中聯(lián)合檢測(cè)p16及CDO1基因甲基化在膠質(zhì)瘤診斷中的意義。方法采用甲基化特異PCR(Methylation-specific PCR,MSP)法分別檢測(cè)49例膠質(zhì)瘤患者腫瘤組織及其對(duì)應(yīng)的術(shù)前血漿、10例顱腦外傷患者挫傷的腦組織及其對(duì)應(yīng)的術(shù)前血漿和16例健康體檢者血漿中p16及半胱氨酸雙加氧酶1(cysteinedioxygenase1,CDO1)基因的甲基化狀態(tài),以探討血漿中檢測(cè)p16及CDO1基因甲基化在膠質(zhì)瘤臨床診斷中的價(jià)值。結(jié)果膠質(zhì)瘤組織中p16及CDO1基因甲基化率分別為53.1%(26/49)、42.9%(21/49),對(duì)應(yīng)的血漿甲基化率為39.0%(19/49)、34.7%(17/49)。在腫瘤組織和血漿中p16及CDO1至少一個(gè)(p16和/或CDO1)基因甲基化的頻率為75.5%(37/49)和67.3%(33/49)。血漿中p16及CDO1基因(甲基化)聯(lián)合檢測(cè)膠質(zhì)瘤的靈敏度為67.3%,較單基因檢測(cè)的靈敏度明顯提高。對(duì)照組中10例腦挫傷組織和26例血漿中均未檢測(cè)出p16和CDO1基因甲基化異常,提示p16及CDO1基因(甲基化)單獨(dú)或聯(lián)合檢測(cè)的特異度都為100%。單因素分析顯示術(shù)前患者血漿中p16和/或CDO1基因甲基化與年齡、性別、腫瘤分級(jí)無關(guān)。結(jié)論對(duì)一些腫瘤特異基因的甲基化狀態(tài)進(jìn)行聯(lián)合檢測(cè)可提高膠質(zhì)瘤在血清學(xué)診斷水平上的靈敏度,為膠質(zhì)瘤的篩查及早期診斷提供新的臨床診斷思路。
[Abstract]:Objective to investigate the significance of combined detection of p16 and CDO1 gene methylation in plasma in the diagnosis of glioma. Methods the tumor tissues of 49 patients with glioma and their corresponding preoperative plasma levels were detected by methylation specific PCR(Methylation-specific polymerase chain reaction (PCR(Methylation-specific) in 10 patients with craniocerebral trauma and their corresponding preoperative plasma and 16 healthy controls. The methylation state of the p16 and cysteine dioxygenase 1 (CDO1) gene, To evaluate the clinical value of detection of p16 and CDO1 gene methylation in plasma for glioma. Results the methylation rates of p16 gene and CDO1 gene in glioma tissues were 53.1% and 42.9%, respectively, and the corresponding plasma methylation rates were 39.0% and 39.0%, respectively. The frequency of methylation of p16 and at least one CDO1 gene in tumor tissues and plasma was 75.5 / 37 / 49) and 67.3 / 49g / 49 respectively. The sensitivity of combined detection of p16 and CDO1 gene (methylation) in plasma was 67.3, which was significantly higher than that of single gene. No abnormal methylation of p16 and CDO1 genes was detected in 10 brain contusion tissues and 26 plasma samples in the control group, suggesting that the specificity of p16 and CDO1 (methylation) alone or in combination was 100%. Univariate analysis showed that plasma p16 and / or CDO1 gene methylation had no correlation with age, sex and tumor grade. Conclusion the combined detection of methylation status of some tumor-specific genes can improve the sensitivity of serological diagnosis of gliomas and provide a new clinical diagnosis method for the screening and early diagnosis of gliomas.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R739.41

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本文編號(hào)：1825998