本文選題：乳腺癌 + 微小RNA-a　； 參考：《中國(guó)臨床藥理學(xué)雜志》2017年16期

【摘要】：目的研究微小RNA-23a(miR-23a)對(duì)乳腺癌細(xì)胞增殖和侵襲的影響。方法將人乳腺癌MCF-7和MDA-MB-435S復(fù)蘇培養(yǎng)后,接種至6孔板中,隨機(jī)分組進(jìn)行培養(yǎng),共分為6組:RNAi-Ⅰ組、過(guò)表達(dá)-Ⅰ組、對(duì)照-Ⅰ組、RNAi-Ⅱ組、過(guò)表達(dá)-Ⅱ組、對(duì)照-Ⅱ組。構(gòu)建MiR-23a重組干擾及過(guò)表達(dá)載體,用Lipofectamine 2000將構(gòu)建成功的MiR-23a重組干擾及過(guò)表達(dá)載體分別轉(zhuǎn)染至各組人乳腺癌MCF-7和MDA-MB-435S細(xì)胞系中。結(jié)果在培養(yǎng)72 h后的OD492值:過(guò)表達(dá)組的MCF-7細(xì)胞和MDA-MB-435S細(xì)胞可分別達(dá)到0.52±0.03和0.44±0.03,而RNAi組MCF-7細(xì)胞和MDA-MB-435S細(xì)胞僅為0.36±0.02和0.31±0.02,與對(duì)照-Ⅰ組和對(duì)照-Ⅱ組比較,RNAi-Ⅰ組和RNAi-Ⅱ組的差異均有統(tǒng)計(jì)學(xué)意義(均P0.05)。過(guò)表達(dá)-Ⅰ組和過(guò)表達(dá)-Ⅱ組的細(xì)胞倍增時(shí)間分別為(26.61±5.23),(29.23±5.51)h,而RNAi-Ⅰ組和RNAi-Ⅱ組的細(xì)胞則分別為(42.24±3.47),(58.17±5.34)h。過(guò)表達(dá)-Ⅰ組和過(guò)表達(dá)-Ⅱ組的M期細(xì)胞比例分別為52.36%,51.59%;而RNAi-Ⅰ組和RNAi-Ⅱ組則分別為76.70%,74.17%。與對(duì)照-Ⅰ組比較,RNAi-Ⅰ組和過(guò)表達(dá)-Ⅰ組的細(xì)胞倍增時(shí)間及M期比例差異均有統(tǒng)計(jì)學(xué)意義(均P0.05)。結(jié)論 MiR-23a的表達(dá)水平與乳腺癌細(xì)胞的增殖和侵襲呈現(xiàn)正相關(guān)性。
[Abstract]:Objective to study the effect of microRNA-23a- miR-23a on the proliferation and invasion of breast cancer cells. Methods Human breast cancer MCF-7 and MDA-MB-435S were resuscitated and inoculated into 6-well plate, and were divided into 6 groups randomly. They were divided into 6 groups: group 鈪，

本文編號(hào)：1820869