本文選題：非小細(xì)胞肺癌 + 白介素6��； 參考：《貴州醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:分析P-選擇素、TPO、IL-6及血小板與非小細(xì)胞肺癌(NSCLC)的相關(guān)性。方法:選取2015年11月至2016年11月期間在貴州醫(yī)科大學(xué)附屬醫(yī)院和貴州醫(yī)科大學(xué)附屬腫瘤醫(yī)院被確診為NSCLC患者50例為NSCLC組,并以同期體檢健康者50例作為對(duì)照組。應(yīng)用酶聯(lián)免疫吸附法(ELISA)和全自動(dòng)血細(xì)胞分析儀分別檢測(cè)患者術(shù)前和術(shù)后3-4周以及健康體檢者的血清P-選擇素、IL-6、TPO濃度以及PLT數(shù)量;免疫組織化學(xué)(IHC)用于檢測(cè)患者術(shù)后癌組織及癌旁組織中P-選擇素、IL-6、TPO的表達(dá)情況。然后應(yīng)用SPSS19.0軟件對(duì)以上指標(biāo)的檢測(cè)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果:NSCLC組的術(shù)前外周血血清P-選擇素、IL-6、TPO、PLT水平均高于健康對(duì)照組(P0.05);術(shù)后外周血血清IL-6、P-選擇素和TPO的濃度明顯低于術(shù)前(P0.05);癌組織中免疫組化IL-6陽性表達(dá)者血清IL-6水平明顯高于陰性患者(P0.05);免疫組化陽性表達(dá)者血清P-選擇素水平高于陰性患者,但差異沒有統(tǒng)計(jì)學(xué)意義(P0.05)。在NSCLC患者中,外周血IL-6、TPO及PLT隨著病情的加重(分期和淋巴結(jié)轉(zhuǎn)移)有升高的趨勢(shì)(P0.05),且與吸煙指數(shù)有關(guān)(P0.05),與病理分型無關(guān)(P0.05);而P-選擇素的水平只與TNM分期和病理分型有關(guān),且Ⅲ、Ⅳ期的患者水平明顯高于Ⅰ、Ⅱ期患者,腺癌患者高于鱗癌而與其他因素?zé)o明顯相關(guān)性。肺癌組織中的IL-6表達(dá)明顯高于癌旁組織(P0.05),并且與吸煙指數(shù)和病情嚴(yán)重程度有相關(guān)性(P0.05),但與腫瘤大小和組織分型無關(guān)(P0.05);肺癌組織中的P-選擇素表達(dá)明顯高于癌旁組織(P0.05),只與病理分期和組織類型有關(guān)(P0.05);TPO在肺癌組織中未見明顯表達(dá)。非小細(xì)胞肺癌組TPO、PLT、IL-6血清水平兩兩之間存在不同程度的相關(guān)性。其中,TPO與PLT的相關(guān)系數(shù)最高。結(jié)論:NSCLC患者體內(nèi)IL-6、TPO、P-選擇素、PLT升高對(duì)病情評(píng)估有一定的臨床意義;NSCLC患者IL-6的升高與IL-6在腫瘤組織中的高表達(dá)有關(guān);IL-6與TPO可能參與了NSCLC患者中血小板數(shù)量的改變。NSCLC患者P-選擇素的表達(dá)水平可以協(xié)助判斷的病理分型、遠(yuǎn)處轉(zhuǎn)移能力及預(yù)后。
[Abstract]:Aim: to analyze the correlation between P-selectin TPO 6-6 and platelets and NSCLC in patients with non-small cell lung cancer (NSCLC). Methods: from November 2015 to November 2016, 50 patients with NSCLC in affiliated Hospital of Guizhou Medical University and affiliated Cancer Hospital of Guizhou Medical University were selected as NSCLC group, and 50 healthy persons as control group. The serum levels of P- selectin IL-6 TPO and the number of PLT were measured by Elisa and automatic blood cell analyzer before and 3 to 4 weeks after operation and 3 to 4 weeks after operation, respectively. Immunohistochemistry was used to detect the expression of P- selectin IL-6 TPO in cancer tissues and paracancerous tissues after operation. Then the SPSS19.0 software is used to carry on the statistical analysis to the above index detection data. Results the serum levels of P- selectin, IL-6, TPO-PLT were higher than those of healthy control group before operation, and the levels of serum IL-6, P- selectin and TPO in postoperative peripheral blood were significantly lower than those before operation, and the levels of serum IL-6 in patients with positive expression of immunohistochemical IL-6 in cancer tissues were significantly lower than those in the control group. The level of serum P- selectin in the patients with positive immunohistochemical expression was higher than that in the patients with negative staining. But the difference was not statistically significant (P 0.05). In patients with NSCLC, the levels of IL-6TPO and PLT in peripheral blood increased with the severity of the disease (stage and lymph node metastasis), and were related to smoking index (P0.05), not to pathological type (P0.05), but the level of P- selectin was only related to TNM stage and pathological type, and 鈪，

本文編號(hào)：1814446