本文選題：納米銀 + 乏氧��； 參考：《南京航空航天大學(xué)》2017年碩士論文

【摘要】：納米銀作為一種抗腫瘤劑,可廣泛應(yīng)用于腫瘤治療。大多數(shù)腫瘤內(nèi)都存在乏氧細胞,其抵抗射線,使治療效果差。同樣,人腦膠質(zhì)瘤內(nèi)缺氧不可避免。之前有體外細胞實驗證實,納米銀能增強人神經(jīng)膠質(zhì)瘤細胞對放射線的敏感性。然而,納米銀對乏氧膠質(zhì)瘤細胞的作用仍不清楚。本文討論了納米銀對乏氧膠質(zhì)瘤U251細胞的影響。采用乏氧裝置體外培養(yǎng)急性乏氧細胞,用CCK-8實驗檢測納米銀對細胞的毒性;隨后,將膠質(zhì)瘤細胞分為對照組、輻射組、單純納米銀組和聯(lián)合治療組。四組分別對細胞凋亡、周期、克隆形成、線粒體膜電位、自噬及活性氧等進行檢測,分析納米銀與乏氧細胞共孵育后,細胞對放射線的敏感性及納米銀、輻照對乏氧細胞自噬的影響。結(jié)果表明:1.用納米銀處理的乏氧膠質(zhì)瘤細胞存在毒性劑量效應(yīng),出于對細胞存活率的考慮,選取15μg/mL的納米銀進行之后研究。2.納米銀對急性乏氧后的U251細胞有放療增敏效果,并找到了治療急性乏氧U251細胞的最佳射線強度。3.使用自噬抑制劑3-MA驗證,納米銀/輻射都能誘導(dǎo)急性乏氧U251細胞自噬,且其引起的自噬對急性乏氧細胞起抑制細胞存活作用。4.同樣條件下,納米銀還引起急性乏氧細胞產(chǎn)生活性氧,造成線粒體膜電位損傷及引發(fā)細胞凋亡。本文結(jié)果顯示,納米銀可有效地增加急性乏氧腦膠質(zhì)瘤對射線的敏感性,對納米銀引起乏氧細胞自噬表達升高及其機制研究具有理論意義,可為納米銀在腫瘤放療領(lǐng)域的應(yīng)用提供依據(jù)。
[Abstract]:As an anti-tumor agent, nano-silver can be widely used in tumor therapy. Hypoxia cells are present in most tumors, which resist radiation and make the treatment ineffective. Similarly, hypoxia in human gliomas is inevitable. Previous in vitro cell experiments have confirmed that silver nanoparticles can enhance the radiosensitivity of human glioma cells to radiation. However, the effect of silver nanoparticles on hypoxic glioma cells remains unclear. The effect of nano silver on hypoxia glioma U251 cells was discussed in this paper. Acute hypoxia cells were cultured in vitro with hypoxia apparatus. The toxicity of silver nanoparticles to cells was detected by CCK-8 assay. Then glioma cells were divided into control group radiation group simple nano-silver group and combined treatment group. Apoptosis, cell cycle, clone formation, mitochondrial membrane potential, autophagy and reactive oxygen species were detected in the four groups. Effects of irradiation on autophagy of hypoxic cells. The result shows that 1: 1. The hypoxic glioma cells treated with nano-silver had toxic dose effect. For the sake of cell survival rate, 15 渭 g/mL nano-silver was selected to carry out the study. Silver nanoparticles can sensitize U251 cells after acute hypoxia and find the best radiation intensity. 3. Using autophagy inhibitor 3-MA, nano-silver / radiation could induce acute hypoxia U251 cell autophagy, and its induced autophagy could inhibit the survival of U251 cells. Under the same conditions, silver nanoparticles also induced reactive oxygen species (Ros) production in acute hypoxia cells, resulting in mitochondrial membrane potential damage and apoptosis. The results show that nano-silver can effectively increase the radiosensitivity of acute hypoxic gliomas, and has theoretical significance in the study of the mechanism of the increase of autophagy expression induced by nano-silver. It can provide the basis for the application of nano silver in tumor radiotherapy.
【學(xué)位授予單位】：南京航空航天大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R730.55

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