本文選題：槲皮素 + 胃癌細(xì)胞��； 參考：《安徽醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:胃癌是常見的消化系統(tǒng)的惡性腫瘤,有很高的發(fā)生率及病死率。上皮間質(zhì)轉(zhuǎn)化(EMT)能夠促進(jìn)腫瘤細(xì)胞的侵襲和轉(zhuǎn)移能力,在腫瘤的發(fā)生和進(jìn)展中具有重要的作用。槲皮素(Quercetin)是一種自然界中廣泛存在的黃酮類化合物,已被證實(shí)具有抗炎、抗氧化、抗腫瘤等多種生理作用。研究證實(shí)槲皮素對(duì)上皮細(xì)胞來(lái)源的惡性腫瘤具有明顯的抑制作用,提示其作為抗腫瘤藥物應(yīng)用的潛在價(jià)值。本研究旨在探討槲皮素對(duì)人胃癌細(xì)胞SGC-7901上皮間質(zhì)轉(zhuǎn)化的作用及可能機(jī)制。方法:胃癌細(xì)胞SGC-7901分為對(duì)照組(普通培養(yǎng)液)、LY294002組(培養(yǎng)液加LY294002)、槲皮素組(培養(yǎng)液加槲皮素);采用MTT法檢測(cè)槲皮素及LY294002對(duì)胃癌細(xì)胞SGC-7901增殖的影響,依據(jù)細(xì)胞的生長(zhǎng)抑制率(inhibition rate,IR)計(jì)算槲皮素及LY294002對(duì)胃癌細(xì)胞SGC-7901的半數(shù)抑制濃度(the median inhibitory concentration,IC50)值,參照IC50值進(jìn)行后續(xù)實(shí)驗(yàn);采用劃痕試驗(yàn)檢測(cè)3組胃癌細(xì)胞SGC-7901增殖遷移能力;采用Western blot檢測(cè)胃癌細(xì)胞AKT、p-AKT、Snail、Vimentin及E-cadherin蛋白表達(dá)情況;采用熒光定量PCR檢測(cè)3組胃癌細(xì)胞上皮間質(zhì)轉(zhuǎn)化相關(guān)因子Snail、Vimentin及E-cadherin m RNA的表達(dá)情況。結(jié)果:槲皮素及LY294002對(duì)胃癌細(xì)胞SGC-7901均有明顯的增增殖抑制作用,槲皮素作用胃癌細(xì)胞IC50為(112.9±2.05)μmol/L,LY294002為(46.35±3.13)μmol/L;LY294002組胃癌細(xì)胞遷移距離[(0.16±0.03)mm]、槲皮素組胃癌細(xì)胞遷移距離[(0.15±0.02)mm]均低于對(duì)照組[(0.44±0.04)mm],LY294002組與槲皮素組胃癌細(xì)胞遷移距離比較差異無(wú)統(tǒng)計(jì)學(xué)意義;Snail、Vimentin蛋白表達(dá)量在LY294002組與槲皮素組均明顯低于對(duì)照組,E-cadherin表達(dá)在LY294002組與槲皮素組高于對(duì)照組;p-AKT蛋白表達(dá)量在LY294002組與槲皮素組均明顯低于對(duì)照組;AKT蛋白表達(dá)量在LY294002組、槲皮素組及對(duì)照組中無(wú)明顯差異;Snail、Vimentin m RNA表達(dá)量在LY294002組、槲皮素組均明顯低于對(duì)照組,E-cadherin m RNA表達(dá)量在LY294002組、槲皮素組高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義);LY294002組Snail、Vimentin、E-cadherin m RNA及蛋白表達(dá)量、p-AKT蛋白表達(dá)量與槲皮素組比較差異均無(wú)統(tǒng)計(jì)學(xué)意義。結(jié)論:槲皮素對(duì)人胃癌細(xì)胞系SGC-7901的上皮間質(zhì)轉(zhuǎn)化具有抑制作用,其作用機(jī)制可能是通過(guò)對(duì)AKT信號(hào)通路的抑制實(shí)現(xiàn)的。
[Abstract]:Objective: gastric cancer is a common malignant tumor of digestive system with high incidence and mortality. Epithelial mesenchymal transformation (EMTT) can promote the invasion and metastasis of tumor cells and play an important role in tumorigenesis and progression. Quercetin (Quercetin) is a kind of flavonoids widely existing in nature, which has been proved to have many physiological effects such as anti-inflammatory, anti-oxidation, anti-tumor and so on. It is confirmed that quercetin has a significant inhibitory effect on malignant tumors derived from epithelial cells, indicating the potential value of quercetin as an antineoplastic drug. The aim of this study was to investigate the effect of quercetin on epithelial stromal transformation of human gastric cancer cell line SGC-7901 and its possible mechanism. Methods: gastric cancer SGC-7901 cells were divided into two groups: normal culture medium LY294002 group (culture medium + LY294002), quercetin group (culture medium + quercetin), MTT assay to detect the effect of quercetin and LY294002 on the proliferation of gastric cancer cell line SGC-7901. According to cell growth inhibition rate (IRR), the median inhibitory concentration of IC50 was calculated by quercetin and LY294002 on gastric cancer cell line SGC-7901, and the SGC-7901 proliferation and migration ability of three groups of gastric cancer cells was detected by scratch test. Western blot was used to detect the expression of Vimentin and E-cadherin protein in gastric cancer cells, and the expression of Snail-Vimentin and E-cadherin m RNA was detected by fluorescence quantitative PCR. Results: quercetin and LY294002 could significantly inhibit the proliferation of gastric cancer cell line SGC-7901. The migration distance of gastric cancer cells in quercetin treated with IC50 112.9 鹵2.05 渭 mol / L LY294002 was 46.35 鹵3.13 渭 mol / L LY294002 [0.16 鹵0.03)mm], while the migration distance [0.15 鹵0.02)mm] of quercetin group was lower than that of control group [0.44 鹵0.04)mm] LY294002 group and quercetin group. There was no significant difference in migration distance between quercetin group and quercetin group. The expression of E-cadherin in LY294002 group and quercetin group was significantly lower than that in LY294002 group and quercetin group. The expression of p-AKT protein in LY294002 group and quercetin group was significantly lower than that in LY294002 group. There was no significant difference in the expression of Vimentin m RNA between the quercetin group and the control group. The expression of E-cadherin m RNA in the quercetin group was significantly lower than that in the LY294002 group, and the expression of Vimentin m RNA in the quercetin group was higher than that in the control group. There was no significant difference in the expression of E-cadherin RNA and protein between LY294002 group and quercetin group. Conclusion: quercetin can inhibit the epithelial interstitial transformation of human gastric cancer cell line SGC-7901, and its mechanism may be through the inhibition of AKT signaling pathway.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 Abolfazl Avan;Ravi Narayan;Elisa Giovannetti;Godefridus J Peters;;Role of Akt signaling in resistance to DNA-targeted therapy[J];World Journal of Clinical Oncology;2016年05期

2 Ammar Natalwala;Robert Spychal;Chris Tselepis;;Epithelial-mesenchymal transition mediated tumourigenesis in the gastrointestinal tract[J];World Journal of Gastroenterology;2008年24期

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本文編號(hào)：1780141