本文選題：乳腺癌 + 循環(huán)腫瘤細(xì)胞��； 參考：《廣西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:研究MMP-11蛋白在乳腺癌中的表達(dá)情況以及外周血循環(huán)腫瘤細(xì)胞在乳腺癌中的臨床意義,探討MMP-11蛋白的表達(dá)與乳腺癌外周血循環(huán)腫瘤細(xì)胞的相關(guān)性。方法:收集2015年7月至2015年12月在廣西醫(yī)科大學(xué)第一附屬醫(yī)院胃腸腺體二病區(qū)新確診為乳腺癌并且未經(jīng)過任何手術(shù)、放療、化療治療的患者137例。在手術(shù)前采集患者外周血10ml,運(yùn)用Canpatrol~(TM) CTCs方法檢測乳腺癌外周血循環(huán)腫瘤細(xì)胞,運(yùn)用免疫組化方法檢測MMP-11蛋白在乳腺癌中的表達(dá)情況。結(jié)果:137例入組病例,MMP-11蛋白在100例乳腺癌組織中表達(dá)陽性,37例表達(dá)陰性,陽性率為72%;MMP-11蛋白的表達(dá)與乳腺癌患者臨床分期具有相關(guān)性(P=0.049);137例乳腺癌患者中112例乳腺癌患者外周血檢測出循環(huán)腫瘤細(xì)胞,陽性率為82%;在137例患者中,總CTCs數(shù)量平均數(shù)為4.73(0-36),上皮型CTCs數(shù)量平均數(shù)為1.99(0-22),混合型CTCs為2.06(0-17),間質(zhì)型CTCs為0.69(0-7);在112例CTCs陽性患者中,三種類型CTCs所占CTCs比例的平均值:上皮型CTCs為48.7%,混合型CTCs為39.8%,間質(zhì)型CTCs為11.5%;乳腺癌外周血總CTCs的陽性檢測結(jié)果與MMP-11蛋白在乳腺癌中的表達(dá)具有相關(guān)性(P=0.009);總CTCs在數(shù)量上與MMP-11蛋白表達(dá)具有相關(guān)性(P=0.042)。結(jié)論:MMP-11在乳腺癌中的表達(dá)與循環(huán)腫瘤細(xì)胞具有相關(guān)性,MMP-11蛋白可能通過改變?nèi)橄侔┠[瘤微環(huán)境促進(jìn)循環(huán)腫瘤細(xì)胞的形成;Canpatrol~(TM) CTCs循環(huán)腫瘤細(xì)胞檢測技術(shù)檢測乳腺癌外周血循環(huán)腫瘤細(xì)胞陽性率為82%,Canpatrol~(TM) CTCs檢測技術(shù)是一種高效CTCs檢測方法。
[Abstract]:Objective: to study the expression of MMP-11 protein in breast cancer and the clinical significance of circulating tumor cells in peripheral blood, and to explore the correlation between the expression of MMP-11 protein and circulating tumor cells in peripheral blood of breast cancer.Methods: from July 2015 to December 2015, 137 patients with breast cancer who were newly diagnosed as breast cancer in the second section of gastrointestinal gland of the first affiliated Hospital of Guangxi Medical University without any operation, radiotherapy and chemotherapy were collected.The peripheral blood of 10 ml patients was collected before operation. The circulating tumor cells in peripheral blood of breast cancer were detected by Canpatrola CTCs method and the expression of MMP-11 protein in breast cancer was detected by immunohistochemical method.Results the positive expression of MMP-11 protein in 100 cases of breast cancer was negative in 37 cases.The positive rate of MMP-11 protein expression was correlated with clinical stage of breast cancer patients. Circulating tumor cells were detected in peripheral blood of 112 breast cancer patients out of 137 breast cancer patients, and the positive rate was 82% in 137 breast cancer patients.The average number of total CTCs, epithelial CTCs, mixed CTCs and interstitial CTCs were 4.73 ~ 0 ~ 36, 1.99 ~ 22 ~ 22, 2.06 ~ 17 ~ 17 and 0.69 ~ 0 ~ 7, respectively.The average proportion of CTCs in three types of CTCs was 48.7 in epithelial type, 39.8 in mixed type and 11.5 in interstitial CTCs. The positive results of total CTCs in peripheral blood of breast cancer were correlated with the expression of MMP-11 protein in breast cancer.There was a correlation between the quantity and the expression of MMP-11 protein.Conclusion the expression of MMP 11 in breast cancer is associated with circulating tumor cells. The expression of MMP 11 protein may promote the formation of circulating tumor cells by changing the microenvironment of breast cancer. (CTCs) circulating tumor cell detection technique may be used to detect the peripheral tumor cells of breast cancer.The positive rate of tumor cells in blood circulation is 82%. The technique of CTCs is an effective method for detecting CTCs.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 Hai-Tao Xu;Jing Miao;Jian-Wei Liu;Lian-Guo Zhang;Qing-Guang Zhang;;Prognostic value of circulating tumor cells in esophageal cancer[J];World Journal of Gastroenterology;2017年07期

2 Henrik Laurell;Michèle Bouisson;Philippe Berthelémy;Philippe Rochaix;Sébastien Déjean;Philippe Besse;Christiana Susini;Lucien Pradayrol;Nicole Vaysse;Louis Buscail;;Identification of biomarkers of human pancreatic adenocarcinomas by expression profiling and validation with gene expression analysis in endoscopic ultrasound-guided fine needle aspiration samples[J];World Journal of Gastroenterology;2006年21期

，

本文編號：1760213