本文選題：膠質(zhì)母細(xì)胞瘤 + RNA-seq��； 參考：《基因組學(xué)與應(yīng)用生物學(xué)》2017年04期

【摘要】：本研究基于RNA-seq數(shù)據(jù)分析了膠質(zhì)母細(xì)胞瘤中的差異表達(dá)基因,并對(duì)差異表達(dá)基因進(jìn)行了功能(GO term)和通路(KEGG)富集分析。進(jìn)一步通過蛋白相互作用網(wǎng)絡(luò)挖掘了膠質(zhì)母細(xì)胞瘤的調(diào)控機(jī)理。結(jié)果表明,405個(gè)基因在腫瘤組織中差異表達(dá)(p-value≤0.05,|log FC|≥1.5),其中216個(gè)(53.3%)基因上調(diào),189個(gè)(46.6%)基因下調(diào)�；虮倔w(gene ontology,GO)富集結(jié)果表明,這些差異表達(dá)基因參與了離子轉(zhuǎn)運(yùn),神經(jīng)沖動(dòng)傳遞,細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)和細(xì)胞粘附等。此外,KEGG通路富集結(jié)果表明,差異基因參與了許多重要的生物學(xué)通路,包括ECM受體相互作用、黏著和鈣信號(hào)等通路。進(jìn)一步的蛋白相互作用網(wǎng)絡(luò)分析鑒定了5個(gè)關(guān)鍵的hub基因,包括PTK2B、CDK1、FN1、CCND1和FOS。這5個(gè)關(guān)鍵基因?qū)τ谀z質(zhì)母細(xì)胞瘤的發(fā)生和發(fā)展可能起到了關(guān)鍵作用,可以作為潛在的調(diào)控位點(diǎn)和篩選的標(biāo)志物。
[Abstract]:In this study, differential expression genes in glioblastoma were analyzed based on RNA-seq data, and the differentially expressed genes were analyzed by functional go and KEGG) enrichment.The regulatory mechanism of glioblastoma was further explored through protein interaction networks.The results showed that the differential expression of p-value was 鈮，

本文編號(hào)：1759135