本文選題：免疫組織化學(xué)染色 + 辣根過(guò)氧化酶��； 參考：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2017年11期

【摘要】：目的通過(guò)對(duì)現(xiàn)有的免疫組織化學(xué)雙重標(biāo)記方法(雙標(biāo))的改進(jìn),探索一種穩(wěn)定且易于辨認(rèn)的顯色方法,便于在普通光學(xué)顯微鏡下觀察肺腺癌組織中腫瘤相關(guān)巨噬細(xì)胞的分布情況。方法改進(jìn)的免疫組織化學(xué)雙標(biāo)染色分別使用二抗鏈接的堿性磷酸酶和辣根過(guò)氧化物酶作為底物顯色酶,底物分別選用固紅(AP-Red)和二氨基聯(lián)苯胺(DAB),在顯微鏡下次序控制雙標(biāo)顯色,觀察肺癌組織中腫瘤相關(guān)巨噬細(xì)胞的分布情況。結(jié)果免疫組織化學(xué)雙重標(biāo)記中第一標(biāo)記AP-Red的紅色與第二標(biāo)記DAB的棕黃色反差大,背景低,易于分辨。兩種顏色標(biāo)記的同一部位呈現(xiàn)磚紅色,易于表示雙標(biāo)部位。統(tǒng)計(jì)顯示在肺腺癌組織標(biāo)本中,以M2型腫瘤相關(guān)巨噬細(xì)胞為主,約占80%。結(jié)論改進(jìn)后的顯色系統(tǒng)建立了簡(jiǎn)便、可靠的免疫組織化學(xué)雙重標(biāo)記新方法。標(biāo)記結(jié)果可以清晰穩(wěn)定顯示腫瘤相關(guān)巨噬細(xì)胞在肺腺癌組織中分布情況,便于進(jìn)一步研究其在肺癌發(fā)生、發(fā)展中的作用。
[Abstract]:Objective to explore a stable and easily recognizable method for color development by improving the existing double labeling method (double labeling) of immunohistochemistry.It is convenient to observe the distribution of tumor-associated macrophages in lung adenocarcinoma tissue under general optical microscope.Methods the modified double labeling immunohistochemical staining was performed by using the linked alkaline phosphatase and horseradish peroxidase as the substrate chromogenic enzyme, and the substrate AP-Red and the diaminobenzidine DABX, respectively. The double standard color was controlled in order under the microscope.The distribution of tumor associated macrophages in lung cancer tissues was observed.Results in the double labeling of immunohistochemistry, the red of the first label AP-Red and the brown yellow of the second label DAB had a big contrast, low background and easy to distinguish.The same part of the two color marks appears brick red, easy to represent the double-marked area.Statistics showed that M 2 tumor associated macrophages were predominant in lung adenocarcinoma tissues, accounting for about 80%.Conclusion the improved color system has established a simple and reliable double labeling method for immunohistochemistry.The results of labeling can clearly and stably show the distribution of tumor associated macrophages in lung adenocarcinoma tissues and facilitate further study of the role of tumor associated macrophages in the genesis and development of lung cancer.
【作者單位】： 第三軍醫(yī)大學(xué)西南醫(yī)院全軍臨床病理學(xué)研究所/西南癌癥中心;
【基金】：國(guó)家重點(diǎn)研發(fā)計(jì)劃重點(diǎn)專(zhuān)項(xiàng)(2016YFC201801) 國(guó)家自然科學(xué)基金面上項(xiàng)目(81372273) 西南醫(yī)院生物強(qiáng)院科技創(chuàng)新計(jì)劃(SWH2016JCZD-04)~~
【分類(lèi)號(hào)】：R734.2

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