本文選題：舌鱗狀細(xì)胞癌　切入點：Oct4　出處：《南昌大學(xué)》2015年碩士論文

【摘要】：背景與目的:腫瘤干細(xì)胞參與腫瘤的發(fā)生及發(fā)展,促進(jìn)腫瘤的復(fù)發(fā)與轉(zhuǎn)移。Oct4、Sox2、Nanog為重要的轉(zhuǎn)錄因子,在維持胚胎干細(xì)胞多能性和自我更新特性方面具有重要作用。Oct4、Sox2、Nanog與誘導(dǎo)多能性干細(xì)胞有關(guān),這表明Oct4、Sox2、Nanog和腫瘤干細(xì)胞有關(guān)。本研究檢測Oct4、Sox2、Nanog在舌鱗狀細(xì)胞癌(Tongue squamous cell carcinoma,TSCC)組織中的表達(dá)并對其表達(dá)水平與TSCC患者臨床病理參數(shù)及生存率的關(guān)系進(jìn)行統(tǒng)計學(xué)分析,探討其在TSCC的發(fā)生、發(fā)展中的作用和臨床意義。材料與方法:收集2005年01月~2009年12月間南昌大學(xué)附屬口腔醫(yī)院口腔頜面外科51例有完整隨訪資料的舌鱗狀細(xì)胞癌患者手術(shù)切除石蠟組織標(biāo)本。采用免疫組織化學(xué)染色法(SP法)進(jìn)行研究,檢測干細(xì)胞轉(zhuǎn)錄調(diào)控因子Oct4、Sox2、Nanog在舌鱗狀細(xì)胞癌組織中表達(dá)情況。結(jié)果采用IBM SPSS20.0軟件進(jìn)行統(tǒng)計學(xué)分析,分析其與TSCC患者臨床病理參數(shù)、生存率之間的關(guān)系及其相互關(guān)系。結(jié)果:Oct4、Sox2、Nanog在TSCC組織中的陽性表達(dá)率分別為:84.3%(43/51)、92.2%(47/51)、80.4%(41/51)。Kruskal—Wallis H test分析發(fā)現(xiàn)Oct4、Sox2、Nanog表達(dá)與TSCC組織學(xué)分級顯著有關(guān)(分別為p=0.0040.01,p=0.0060.01,p=0.0020.01),Oct4、Sox2、Nanog表達(dá)與TSCC患者臨床病理參數(shù)如年齡、性別、T分期、吸煙史及飲酒史無關(guān)(p0.05),但Oct4表達(dá)與淋巴結(jié)轉(zhuǎn)移顯著有關(guān)(p=0.0010.01),Sox2、Nanog表達(dá)均與TSCC患者淋巴結(jié)轉(zhuǎn)移無關(guān)(p0.05)。Speaman等級相關(guān)分析發(fā)現(xiàn)舌鱗狀細(xì)胞癌組織中Oct4與Sox2表達(dá)呈顯著正相關(guān)(p0.001),Sox2與Nanog表達(dá)無相關(guān)性(p=0.4020.05),Oct4與Nanog表達(dá)呈顯著正相關(guān)(p=0.0020.01)。Kaplan-Meier分析表明Oct4、Sox2、Nanog表達(dá)均與TSCC患者的總體生存率顯著有關(guān)(分別為p=0.0010.01,p=0.0020.01,p=0.0060.01),Oct4、Sox2、Nanog表達(dá)越高,患者的總體生存率越低。在TSCC患者中Oct4、Sox2、Nanog三者同時高表達(dá)的病例總體生存率最低(p=0.0010.01)。多因素Cox比例風(fēng)險回歸模型表明Sox2、Nanog、淋巴結(jié)轉(zhuǎn)移可作為與TSCC患者總體生存期有關(guān)的獨立預(yù)后因素(分別為p=0.0270.05,p=0.0400.05,p=0.0020.01)。結(jié)論:Oct4、Sox2、Nanog表達(dá)與TSCC組織學(xué)分級顯著有關(guān),這表明Oct4、Sox2、Nanog與TSCC細(xì)胞的分化有關(guān)。舌鱗狀細(xì)胞癌組織中Oct4與Sox2表達(dá)呈顯著正相關(guān),Oct4與Nanog表達(dá)呈顯著正相關(guān),這表明Oct4、Sox2、Nanog在舌鱗狀細(xì)胞癌中協(xié)調(diào)發(fā)揮作用,聯(lián)合檢測Oct4、Sox2、Nanog更能確切反映舌鱗狀細(xì)胞癌的生物學(xué)行為。Sox2、Nanog、淋巴結(jié)轉(zhuǎn)移可作為與TSCC患者總體生存期有關(guān)的獨立預(yù)后因素,這表明Sox2、Nanog表達(dá)可能是評估TSCC患者預(yù)后的重要指標(biāo)。
[Abstract]:Background & AIM: tumor stem cells are involved in the occurrence and development of tumor and promote the recurrence and metastasis of tumor. Oct4 Sox2Nanog is an important transcription factor.Oct4N Sox2 Nanog is associated with induced pluripotent stem cells, which suggests that Oct4G Sox2 Nanog is related to tumor stem cells.The purpose of this study was to detect the expression of Oct4G Sox2Nanog in tongue squamous cell carcinoma of tongue, and to analyze the relationship between the expression level and clinicopathological parameters and survival rate of TSCC patients, and to explore its role in the occurrence, development and clinical significance of TSCC.Materials and methods: from January 2005 to December 2009, 51 patients with tongue squamous cell carcinoma underwent surgical excision of paraffin tissue in oral and maxillofacial surgery, Nanchang University affiliated Stomatology Hospital.Immunohistochemical staining was used to study the expression of Oct4G Sox2 Nanog in tongue squamous cell carcinoma (TSCC).Results IBM SPSS20.0 software was used to analyze the relationship between TSCC and clinicopathological parameters and survival rate.緇撴灉:Oct4,Sox2,Nanog鍦═SCC緇勭粐涓殑闃蟲，

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