本文選題：結(jié)直腸癌　切入點(diǎn)：基質(zhì)金屬蛋白酶-14　出處：《石河子大學(xué)》2017年碩士論文

【摘要】：目的:探討基質(zhì)金屬蛋白酶-14(matrixmet-alloproteinase-14 MMP-14)和CD31標(biāo)記的微血管密度(CD31-microvascular density)在結(jié)直腸癌組織中的表達(dá)及其與腫瘤侵襲轉(zhuǎn)移的臨床意義。簡要分析MMP-14和CD31-MVD與結(jié)直腸癌腫瘤發(fā)生、發(fā)展以及侵襲轉(zhuǎn)移的相關(guān)性,以期為今后結(jié)直腸癌患者的治療以及預(yù)后提供一定的指導(dǎo)依據(jù)。方法:1.采用免疫組織化學(xué)方法檢測60例結(jié)直腸癌患者的癌組織及20例正常切緣組織中MMP-14和CD31-MVD的表達(dá)并分析兩者與結(jié)直腸癌患者臨床病理資料的關(guān)系。2.采用q RT-PCR的方法檢測20例新鮮結(jié)直腸癌組織及正常切緣組織中MMP-14m RNA的相對表達(dá)量并分析其與結(jié)直腸癌的關(guān)系。3.采用SPSS17.0統(tǒng)計(jì)軟件對數(shù)據(jù)進(jìn)行統(tǒng)計(jì)分析,結(jié)合臨床病理資料分析MMP-14和CD31與結(jié)直腸腫瘤之間的相關(guān)性,并探討兩者的臨床意義。結(jié)果:1.結(jié)直腸癌組織中的MMP-14陽性表達(dá)率以及CD31-MVD值明顯高于正常切緣組織(P0.05)。2.MMP-14的表達(dá)及CD31-MVD值與腫瘤大小、浸潤深度、TNM分期和淋巴結(jié)轉(zhuǎn)移有相關(guān)性(P0.05),而與患者性別、年齡無明顯相關(guān)(P0.05)。3.MMP-14的表達(dá)與CD31-MVD呈正相關(guān)(P0.05)。4.結(jié)直腸癌組織中MMP-14m RNA的相對表達(dá)量顯著高于正常切緣組織(P0.01)。結(jié)論:結(jié)直腸癌組織中MMP-14的高表達(dá)和CD31標(biāo)記的腫瘤組織微血管密度與結(jié)直腸癌的發(fā)生、浸潤、轉(zhuǎn)移有密切關(guān)系。檢測MMP-14和CD31-MVD值對結(jié)直腸癌患者的臨床治療及預(yù)后有一定的指導(dǎo)意義。
[Abstract]:Objective: to investigate the expression of matrix metalloproteinase-14 (MMP-14) and microvascular density (CD31-microvascular density) labeled by CD31 in colorectal carcinoma and its clinical significance in tumor invasion and metastasis.To analyze the correlation between MMP-14 and CD31-MVD and the occurrence, development, invasion and metastasis of colorectal cancer, so as to provide some guidance for the treatment and prognosis of colorectal cancer patients in the future.Method 1: 1.Immunohistochemical method was used to detect the expression of MMP-14 and CD31-MVD in 60 cases of colorectal cancer and 20 cases of normal incisor tissues and to analyze the relationship between them and clinicopathological data of colorectal cancer.Q RT-PCR method was used to detect the relative expression of MMP-14m RNA in 20 fresh colorectal cancer tissues and normal incisal margin tissues and to analyze the relationship between MMP-14m RNA and colorectal cancer.The correlation between MMP-14, CD31 and colorectal neoplasms was analyzed by SPSS17.0 software and the clinicopathological data. The clinical significance of the two data was discussed.The result is 1: 1.The positive expression rate of MMP-14 and CD31-MVD in colorectal cancer tissue were significantly higher than those in normal incisal margin tissue (P 0.05N. 2.MMP-14). The expression of CD31-MVD was correlated with tumor size, depth of invasion and lymph node metastasis.There was no significant correlation between age and CD31-MVD.The relative expression of MMP-14m RNA in colorectal cancer was significantly higher than that in normal incisal tissue (P 0.01).Conclusion: the high expression of MMP-14 and the microvessel density of tumor tissue labeled with CD31 are closely related to the occurrence, invasion and metastasis of colorectal carcinoma.The detection of MMP-14 and CD31-MVD values has certain guiding significance for the clinical treatment and prognosis of colorectal cancer patients.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R735.34

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